Open Close
General Information
Symbol
Dmel\CycAS.hs
Species
D. melanogaster
Name
FlyBase ID
FBal0065307
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A CycA cDNA lacking sequences encoding the first 169 amino acids (which includes the destruction box) is expressed under the control of a heat shock promoter.

A deleted form of CycA (lacking amino acids 2 to 170) is expressed under the control of an Hsp70 promoter. The deletion removes the "destruction box" and results in a stable CycA protein being produced.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

anaphase & condensed nuclear chromosome

metaphase & condensed nuclear chromosome

Detailed Description
Statement
Reference

Heatshock induced embryos exhibit arrest at metaphase. After expression in stage G2 of cell cycle 14, embryos successfully, albeit slowly, transit mitosis 14 and arrest in mitosis 15. Although most cells arrest in metaphase, about 10% of cells have a prolonged metaphase followed by anaphase. Chromosome disjunction, almost instantaneous in wild-type, is greatly prolonged by the presence of CycAS.hs. Anaphase progression is also disturbed movements are slower, anaphase separation is incomplete. These cells go on to exit mitosis without showing evidence of a later arrest point.

Expression of CycAhs.PK using a 10 minute heat shock during G1 induces S phase in epidermal cells of embryos carrying one copy of CycAS.hs.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

Co-induction of cdc2hs.PS and CycAS.hs leads to a more metaphase arrest than seen in CycAS.hs embryos alone.

Expression of both CycAS.hs and CycBS.hs in embryos using heat shock results in most epidermal cells being arrested in mitosis 2 hours after the heat shock. No more than 2 centrosomes are seen at each spindle pole. All centriole pairs are disengaged and the centrioles are not associated with procentrioles.

The efficiency of S phase induction in embryos is dramatically increased if CycAS.hs is co-expressed with cdc2AF.hs using heat shock.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (6)