Open Close
General Information
Symbol
Dmel\CycB3Δ
Species
D. melanogaster
Name
FlyBase ID
FBal0048166
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Deletion of an internal PsiI fragment, amino acids 19 to 110.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of homozygous CycB3Δ at the permissive temperature prevents or severely delays cell cycle progression at late anaphase.

When CycB3Δ is induced during the G2 phase of cycle 14, cytokinesis does progress albeit slowly to completion and initiation is delayed.

Heatshock expression of CycB3Δ produces embryos with arrested cells with condensed chromosomes at the poles. Cells proceed through the metaphase/anaphase transition with normal timing and separate their chromosomes with kinetics similar to wild-type, but then arrest with condensed chromosomes at the poles. This remains stable for at least 20 minutes in most cases. The separation of the spindle poles during anaphase B occurs as normal, however the organisation of the midbody is abnormal. Cells either lack the compacted foci of tubulin of the normal midbody, or showed foci lacking normal symmetry and organisation. Sometimes a more dynamic arrest is seen in which individual chromosomes leave the spindle pole and travel back along the spindle at rates comparable to anaphase rates. Occasionally, these chromosomes reach the opposite pole. Often, in cells where this phenomenon occurs, the spindle rotates in the plane of the epithelium. Cytogenetic furrow formation appears to proceed normally.

Expression causes a mitotic block in late anaphase (after sister chromosome separation).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Enhancer of
Statement
Reference
NOT Suppressor of
Other
Phenotype Manifest In
NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

Coexpression of three transgenes, cdc2AF.hs, CycBΔ90, and CycB3Δ, restores the entry of epidermal cells in CycAC8LR1 embryos into mitosis 16. After entry into mitosis, the epidermal cells are unable to complete this division.

Coexpression of the transgenes, cdc2hs.PS, CycBΔ90, and CycB3Δ, does not restore the entry of epidermal cells in CycAC8LR1 embryos into mitosis 16. Likewise, coexpression of stghs.PE2, CycBΔ90 and CycB3Δ does not suppress the phenotype.

In CycB3Δ, pbl2 mutant embryos cytokinesis furrows are absent (or exceptional(. Moreover, late spindles are broad and short.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
CycB3Δ
Name Synonyms
Secondary FlyBase IDs
    References (6)