FlyBase Allele Report: Dmel\CycA[S.hs]

General Information

Symbol

Dmel\CycA^S.hs

Species

D. melanogaster

Name

FlyBase ID

FBal0065307

Feature type

allele

Associated gene

Dmel\CycA

Associated Insertion(s)

Carried in Construct

P{HS-CycA^S}

Key Links

Transgenic product class

inframe_deletion

(Sprenger et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Sprenger et al., 1997)

Mutagen

in vitro construct

(Su et al., 1998, Sprenger et al., 1997, Su and O'Farrell, 1997)

Progenitor genotype

Carried in construct

P{HS-CycA^S}

Cytology

Description

A CycA cDNA lacking sequences encoding the first 169 amino acids (which includes the destruction box) is expressed under the control of a heat shock promoter.

(Sprenger et al., 1997)

A deleted form of CycA (lacking amino acids 2 to 170) is expressed under the control of an Hsp70 promoter. The deletion removes the "destruction box" and results in a stable CycA protein being produced.

(Su and O'Farrell, 1997)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

CycA

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased occurrence of cell division | embryonic stage

(Sprenger et al., 1997)

Phenotype Manifest In

anaphase & condensed nuclear chromosome

(Parry and O'Farrell, 2001)

cell cycle

(Parry and O'Farrell, 2001)

embryonic epidermis

(Sprenger et al., 1997)

metaphase & condensed nuclear chromosome

(Parry and O'Farrell, 2001)

Detailed Description

Statement

Reference

Heatshock induced embryos exhibit arrest at metaphase. After expression in stage G2 of cell cycle 14, embryos successfully, albeit slowly, transit mitosis 14 and arrest in mitosis 15. Although most cells arrest in metaphase, about 10% of cells have a prolonged metaphase followed by anaphase. Chromosome disjunction, almost instantaneous in wild-type, is greatly prolonged by the presence of CycA^S.hs. Anaphase progression is also disturbed movements are slower, anaphase separation is incomplete. These cells go on to exit mitosis without showing evidence of a later arrest point.

(Parry and O'Farrell, 2001)

Expression of CycA^hs.PK using a 10 minute heat shock during G1 induces S phase in epidermal cells of embryos carrying one copy of CycA^S.hs.

(Sprenger et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

CycA^S.hs, CycB^S.hs has abnormal mitotic cell cycle phenotype

(Vidwans et al., 1999)

Cdk1^AF.hs, CycA^S.hs has increased occurrence of cell division | embryonic stage phenotype

(Sprenger et al., 1997)

Phenotype Manifest In

Enhanced by

Statement

Reference

CycA^S.hs has cell cycle phenotype, enhanceable by Cdk1^hs.PS

(Parry and O'Farrell, 2001)

Other

Statement

Reference

CycA^S.hs, CycB^S.hs has mitotic cell cycle phenotype

(Vidwans et al., 1999)

Cdk1^AF.hs, CycA^S.hs has embryonic epidermis phenotype

(Sprenger et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

Co-induction of cdc2^hs.PS and CycA^S.hs leads to a more metaphase arrest than seen in CycA^S.hs embryos alone.

(Parry and O'Farrell, 2001)

Expression of both CycA^S.hs and CycB^S.hs in embryos using heat shock results in most epidermal cells being arrested in mitosis 2 hours after the heat shock. No more than 2 centrosomes are seen at each spindle pole. All centriole pairs are disengaged and the centrioles are not associated with procentrioles.

(Vidwans et al., 1999)

The efficiency of S phase induction in embryos is dramatically increased if CycA^S.hs is co-expressed with cdc2^AF.hs using heat shock.

(Sprenger et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

A^S

(Su and O'Farrell, 1997)

CycA^S.hs

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

Open Close