FlyBase Allele Report: Dmel\Raf[K497M.UAS]

Expression of phl^{K497M.Scer\UAS} under the control of Scer\GAL4^amn-c651, results in pupae and adults that are larger than wild-type controls. The wing epidermis of these flies is increased, suggesting that most of this increased size results from increased cell size rather than cell number. Expression of this transgene under the control of Scer\GAL4^amn-X8 causes an increase in pupal length. Flies that express one copy of phl^{K497M.Scer\UAS}, under the control of one copy of either Scer\GAL4^amn-c651 or Scer\GAL4^amn-X8, pupariate about 36 hours after wild-type controls, which results mostly from a prolonged third instar stage. Expression of phl^{K497M.Scer\UAS} under the control of Scer\GAL4^amn-c651 has no effect on prothoracic gland cell size.

(Caldwell et al., 2005)

When phl^{K497M.Scer\UAS} is driven by Scer\GAL4^dpp.blk1 no effect is seen on wing disc growth.

(Garoia et al., 2005)

phl^{K497M.Scer\UAS}; Scer\GAL4^prd.RG1 embryos do not have obvious cuticle phenotypes.

(Urban et al., 2004)

phl^{K497M.Scer\UAS}; Scer\GAL4^{Ubx-lac1-Gal4} adults exhibit wing duplication in the posterior compartment and partial loss of notum and/or hinge. Occasionally pharate adults die within the pupal case, with severe loss of notum tissue. In larvae of this genotype, the wing discs show altered morphology, particularly overgrowth in the posterior hinge and mesonotum. Marker analysis indicates formation a new wing pouch complete with the dorso-ventral boundary, and that pattern duplication is associated with loss of notum, confirming notum/hinge-to-wing transformations.

(Pallavi and Shashidhara, 2003)

When expression is driven by Scer\GAL4^C-765, anterior supraalar bristle and posterior supraalar bristle bristles are usually eliminated and approximately 45% of heminota show an extra dorsocentral bristle. When expression is driven by Scer\GAL4^sca-C253, only the anterior supraalar bristle is eliminated and only occasionally duplicates a dorsocentral bristle. When expression is driven by Scer\GAL4^pnr-MD237, dorsocentral bristles are absent. Expression driven by Scer\GAL4^179b causes lethality and the few pharate adults recovered lack 50-80% of notum macrochaetae, though in half of the cases the socket of the missing bristle was present.

(Culi et al., 2001)

When expression is driven by Scer\GAL4^twi.PB the number of HVM cells is reduced.

(San Martin and Bate, 2001)

phl^{K497M.Scer\UAS}, when driven by Scer\GAL4^en-e16E leads to a posterior to anterior transformation of the wing margin. Overexpression of phl^{K497M.Scer\UAS} in clones driven by Scer\GAL4^Act5C.PI and induced at 36+-12h after egg laying can produce mirror image duplications of the wing and notum to wing transformations.

(Baonza et al., 2000)

When expression is driven by Scer\GAL4^en-e16E the wing is reduced in size and veins L4 and L5 are absent.

(Sotillos and Campuzano, 2000)

Expression of phl^{K497M.Scer\UAS} under the control of Scer\GAL4^sca-537.4 results in flies that lack pieces of wing veins L3 and L4. Ectopic veins are also seen.

(Martin-Blanco et al., 1999)

External Data

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Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Raf^K497M.UAS, Scer\GAL4^en-e16E has visible phenotype, suppressible | partially by Rac1^UAS.cLa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Raf^K497M.UAS, Scer\GAL4^en-e16E has visible phenotype, suppressible | partially by tum^ΔE1E.UAS, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

NOT Enhancer of

Statement

Reference

Raf^K497M.UAS, Scer\GAL4^NP7397 is a non-enhancer of increased cell number | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^NP7397

(Jiang and Edgar, 2009)

Raf^K497M.UAS/Scer\GAL4^Bx-MS1096 is a non-enhancer of abnormal planar polarity | somatic clone phenotype of ft^k07918

(Garoia et al., 2005)

Suppressor of

Statement

Reference

Raf^K497M.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL is a suppressor of hyperplasia | conditional | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

Raf^K497M.UAS/Scer\GAL4^dpp.blk1 is a suppressor of increased cell number phenotype of ft^G-rv

(Garoia et al., 2005)

Raf^K497M.UAS/Scer\GAL4^Bx-MS1096 is a suppressor of increased cell number | somatic clone phenotype of ft^k07918

(Garoia et al., 2005)

NOT Suppressor of

Statement

Reference

Raf^K497M.UAS, Scer\GAL4^NP7397 is a non-suppressor of increased cell number | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^NP7397

(Jiang and Edgar, 2009)

Raf^K497M.UAS/Scer\GAL4^Bx-MS1096 is a non-suppressor of abnormal planar polarity | somatic clone phenotype of ft^k07918

(Garoia et al., 2005)

Other

Statement

Reference

Raf^K497M.UAS, Scer\GAL4^dpp.blk1, ft^G-rv has increased cell death phenotype

(Garoia et al., 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

Raf^K497M.UAS, Scer\GAL4^en-e16E has wing | ectopic | somatic clone phenotype, suppressible by wg^-C.UAS, Scer\GAL4^en-e16E

(Baonza et al., 2000)

Raf^K497M.UAS, Scer\GAL4^en-e16E has wing vein L5 phenotype, suppressible | partially by Rac1^UAS.cLa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Raf^K497M.UAS, Scer\GAL4^en-e16E has wing vein L5 phenotype, suppressible | partially by tum^ΔE1E.UAS, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Raf^K497M.UAS, Scer\GAL4^en-e16E has wing phenotype, suppressible | partially by Rac1^UAS.cLa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Raf^K497M.UAS, Scer\GAL4^en-e16E has wing phenotype, suppressible | partially by tum^ΔE1E.UAS, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Raf^K497M.UAS, Scer\GAL4^en-e16E has wing vein L4 phenotype, suppressible | partially by Rac1^UAS.cLa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Raf^K497M.UAS, Scer\GAL4^en-e16E has wing vein L4 phenotype, suppressible | partially by tum^ΔE1E.UAS, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

NOT Enhancer of

Statement

Reference

Raf^K497M.UAS, Scer\GAL4^NP7397 is a non-enhancer of adult midgut progenitor cell | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^NP7397

(Jiang and Edgar, 2009)

Raf^K497M.UAS/Scer\GAL4^dpp.blk1 is a non-enhancer of wing disc phenotype of ft^G-rv

(Garoia et al., 2005)

Raf^K497M.UAS/Scer\GAL4^ey.PH is a non-enhancer of interommatidial bristle | increased number | somatic clone phenotype of ft^G-rv

(Garoia et al., 2005)

Suppressor of

Statement

Reference

Raf^K497M.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL is a suppressor of adult Malpighian tubule | conditional | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

Raf^K497M.UAS/Scer\GAL4^dpp.blk1 is a suppressor of eye disc phenotype of ft^G-rv

(Garoia et al., 2005)

Raf^K497M.UAS/Scer\GAL4^ey.PH is a suppressor of eye | somatic clone phenotype of ft^G-rv

(Garoia et al., 2005)

NOT Suppressor of

Statement

Reference

Raf^K497M.UAS, Scer\GAL4^NP7397 is a non-suppressor of adult midgut progenitor cell | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^NP7397

(Jiang and Edgar, 2009)

Raf^K497M.UAS/Scer\GAL4^dpp.blk1 is a non-suppressor of wing disc phenotype of ft^G-rv

(Garoia et al., 2005)

Raf^K497M.UAS/Scer\GAL4^ey.PH is a non-suppressor of interommatidial bristle | increased number | somatic clone phenotype of ft^G-rv

(Garoia et al., 2005)

Other

Statement

Reference

Raf^K497M.UAS, Scer\GAL4^ey.PH, ft^G-rv has eye | somatic clone phenotype

(Garoia et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Dis3^KK101473 and Raf^{K497M.Scer\UAS} driven by Scer\GAL4^dpp.PU does not lead to tissue overgrowth in eye-antennal imaginal or wing discs.

(Snee et al., 2016)

Hyperplasia in adult Malpighian tubule clones expressing Ras85D^V12.Scer\UAS (using the MARCM system, under the control of Scer\GAL80^αTub84B.PL and Scer\GAL4^{Scer\FRT.Act5C}) is suppressed by co-expression of phl^{K497M.Scer\UAS}.

(Zeng et al., 2010)

Co-expression of phl^{K497M.Scer\UAS} with Ras85D^V12.Scer\UAS via Scer\GAL4^esg-NP7397 results in a phenotype similar to that of Ras85D^V12.Scer\UAS overexpression alone.

(Jiang and Edgar, 2009)

When phl^{K497M.Scer\UAS} is driven by Scer\GAL4^dpp.blk1 in a ft^G-rv background a suppression is seen in the ft^G-rv overgrowth phenotype in the eye disc, but no effect is seen in the wing disc. An increase in cell non-autonomous cell death is seen in these discs. When phl^{K497M.Scer\UAS} is driven by Scer\GAL4^ey.PH in ft^G-rv mutant eyes, eyes are significantly smaller than wild-type. However eye bristle duplications are still seen.

(Garoia et al., 2005)

Denticle belt fusions in the cuticles of rho^7M43; ru¹ double homozygous embryos are enhanced by phl^{K497M.Scer\UAS}; Scer\GAL4^prd.RG1.

(Urban et al., 2004)

phl^{K497M.Scer\UAS}/wg^-C.Scer\UAS double mutant clones (driven by Scer\GAL4^en-e16E) covering the whole of the posterior compartment of the wing disc show a partial reduction in the size of the wing pouch and notum, and a suppression of the notum to wing transformations seen in phl^{K497M.Scer\UAS} clones.

(Baonza et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

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Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Raf^{K497M.Scer\UAS}

Raf^K497M.UAS

Raf^K497M

(Caldwell et al., 2005)

UAS-KMRaf3.1

(Roch et al., 1998)

phl^{K497M.Scer\UAS}

phl^K497M.UAS

Name Synonyms

Secondary FlyBase IDs

References (21)

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