Expression of phlK497M.Scer\UAS under the control of Scer\GAL4amn-c651, results in pupae and adults that are larger than wild-type controls. The wing epidermis of these flies is increased, suggesting that most of this increased size results from increased cell size rather than cell number. Expression of this transgene under the control of Scer\GAL4amn-X8 causes an increase in pupal length. Flies that express one copy of phlK497M.Scer\UAS, under the control of one copy of either Scer\GAL4amn-c651 or Scer\GAL4amn-X8, pupariate about 36 hours after wild-type controls, which results mostly from a prolonged third instar stage. Expression of phlK497M.Scer\UAS under the control of Scer\GAL4amn-c651 has no effect on prothoracic gland cell size.
phlK497M.Scer\UAS; Scer\GAL4Ubx-lac1-Gal4 adults exhibit wing duplication in the posterior compartment and partial loss of notum and/or hinge. Occasionally pharate adults die within the pupal case, with severe loss of notum tissue. In larvae of this genotype, the wing discs show altered morphology, particularly overgrowth in the posterior hinge and mesonotum. Marker analysis indicates formation a new wing pouch complete with the dorso-ventral boundary, and that pattern duplication is associated with loss of notum, confirming notum/hinge-to-wing transformations.
When expression is driven by Scer\GAL4C-765, anterior supraalar bristle and posterior supraalar bristle bristles are usually eliminated and approximately 45% of heminota show an extra dorsocentral bristle. When expression is driven by Scer\GAL4sca-C253, only the anterior supraalar bristle is eliminated and only occasionally duplicates a dorsocentral bristle. When expression is driven by Scer\GAL4pnr-MD237, dorsocentral bristles are absent. Expression driven by Scer\GAL4179b causes lethality and the few pharate adults recovered lack 50-80% of notum macrochaetae, though in half of the cases the socket of the missing bristle was present.
When expression is driven by Scer\GAL4twi.PB the number of HVM cells is reduced.
When expression is driven by Scer\GAL4en-e16E the wing is reduced in size and veins L4 and L5 are absent.