FlyBase Allele Report: Dmel\Wnt4[UAS.cGa]

Expression of Wnt4^Scer\UAS.cGa under the control of Scer\GAL4^bap.3 in the visceral mesoderm is sufficient to redirect the salivary glands ventrally, away from the visceral mesoderm. The redirection affects the entire salivary gland.

(Harris and Beckendorf, 2007)

Expression of Wnt4^Scer\UAS.cGa under the control of Scer\GAL4^upd-E132 does not result in obvious planar polarity defects in the developing eye discs or adult eyes.

Expression of Wnt4^Scer\UAS.cGa under the control of Scer\GAL4^bi-omb-Gal4 does not result in any polarity changes in the eye disc.

Flies expressing Wnt4^Scer\UAS.cGa under the control of Scer\GAL4^dpp.blk1 or Scer\GAL4^ptc-559.1 show frequent loss of the anterior crossvein. In addition, there are dramatic hair polarity changes in the distal region of the wing, with wing hairs oriented towards the anterior-posterior boundary between veins L3 and L4.

Expression of Wnt4^Scer\UAS.cGa under the control of Scer\GAL4^Act5C.PP in clones in the wing results in wing hairs around the clone shifting their polarity towards the clone.

(Lim et al., 2005)

When Wnt4^Scer\UAS.cGa is driven in clones (when driven by Scer\GAL4^αTub84B.PC) in the wing no alterations in polarity or other effects in the wing are seen. When Wnt4^Scer\UAS.cGa is driven by Scer\GAL4^ptc-559.1 in the adult abdomen at high temperature wing patterning is occasionally altered. When Wnt4^Scer\UAS.cGa is driven by Scer\GAL4^en-e16E polarity defects are seen in the wing. Within the anterior compartment the hairs posterior to vein II tend to turn clockwise to point posteriorly.

(Lawrence et al., 2002)

Expression of Wnt4^Scer\UAS.cGa under the control of Scer\GAL4^ptc-559.1 at 18^oC results in flies with outspread wings. Most animals expressing Wnt4^Scer\UAS.cGa under the control of Scer\GAL4^ptc-559.1 at 29^oC until mid-third larval instar and then shifted to 18^oC die as pharate adults, but some escapers with small ectopic wings arising from the posterior region of the notum near the boundary between the dorsal and ventral notal structures are seen. Parts of the scutellum are lost in these animals. Animals expressing Wnt4^Scer\UAS.cGa under the control of Scer\GAL4^ptc-559.1 at 29^oC throughout development develop ectopic wings of different sizes. Most of the ectopic wings are small and show hair-like structures characteristic of the alula, but large ectopic wings which arise from both sides of the posterior notum are reproducibly obtained. The ectopic wing is a mirror image duplication of the endogenous wing. The ectopic wing usually has wing margin bristles of posterior identity. Anterior structures such as anterior margin bristles and campaniform sensory neurons are occasionally formed. Occasionally, complete wing duplication is seen, this phenotype being associated with almost complete loss of the notum. Expression of Wnt4^Scer\UAS.cGa under the control of Scer\GAL4^ptc-559.1 at 29^oC induces duplication of the halteres. The bifurcation occurs more or less distally, so that the two resulting appendages are either completely separated, form a common scabellum or have fused pedicels. Wing discs expressing Wnt4^Scer\UAS.cGa under the control of Scer\GAL4^ptc-559.1 show outgrowth from the posterior region of the notum. Expression of Wnt4^Scer\UAS.cGa under the control of Scer\GAL4^ptc-559.1 results in extra sternopleural bristles and extra dorsocentral, posterior postalar and scutellar bristles when the scutellum is not transformed into wing.

(Gieseler et al., 2001)

Expression of Wnt4^Scer\UAS.cGa under the control of Scer\GAL4^da.G32 has a variable effect on the cells of the dorsal epidermis in the embryo (in wild-type embryos each parasegment contains 4 types of cells in the dorsal epidermis; the 1^o, 2^o and 3^o cells in the anterior half of the parasegment and the 4^o cells in the posterior half of the parasegment). When one copy of Wnt4^Scer\UAS.cGa is expressed at 29^oC, 21% of segments have a 2^o-3^o-4^o pattern, in which 1^o cells are missing and 3^o cells are expanded. In contrast, 62% of segments show either a 1^o-3^o-4^o or 3^o-4^o pattern (1^o and 3^o cells are difficult to distinguish). Lower levels of expression result in a higher percentage of embryos with a pattern that is more clearly 1^o-3^o-4^o along the dorsal midline, since the 2^o cell fate is still apparent laterally.

(Buratovich et al., 2000)

Embryos expressing one copy of Wnt4^Scer\UAS.cGa under the control of Scer\GAL4^da.G32 show mild but consistent defects consisting of ectopic denticles within the regions of naked cuticle. Posterior segments are more affected than anterior ones and segmentation and diversity of denticle morphology are not entirely lost. Embryos expressing two copies of Wnt4^Scer\UAS.cGa under the control of Scer\GAL4^da.G32 have stronger phenotypes; the naked cuticle is often completely lost and replaced with denticle decorated cuticle, especially in the most posterior segments.

(Gieseler et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Wnt4^UAS.cGa/Scer\GAL4^bi-omb-Gal4 is an enhancer of abnormal planar polarity phenotype of Scer\GAL4^bi-omb-Gal4, fj^UAS.cZa

(Lim et al., 2005)

NOT Enhancer of

Statement

Reference

Scer\GAL4^C587/Wnt4^UAS.cGa is a non-enhancer of increased size | oogenesis phenotype of Scer\GAL4^C587, dlp^UAS.cBa

(Waghmare et al., 2020)

Suppressor of

Statement

Reference

Wnt4^UAS.cGa/Scer\GAL4^dpp.blk1 is a suppressor of abnormal planar polarity phenotype of Scer\GAL4^dpp.blk1, fz^UAS.cAa

(Lim et al., 2005)

Scer\GAL4^ptc-559.1/Wnt4^UAS.cGa is a suppressor | partially of visible phenotype of wg^l-16/wg^l-17

(Gieseler et al., 2001)

NOT Suppressor of

Statement

Reference

Scer\GAL4^C587/Wnt4^UAS.cGa is a non-suppressor of increased size | oogenesis phenotype of Scer\GAL4^C587, dlp^UAS.cBa

(Waghmare et al., 2020)

Other

Statement

Reference

Scer\GAL4^dpp.blk1, Wnt4^UAS.cGa, fj^UAS.cZa has abnormal planar polarity phenotype

(Lim et al., 2005)

Phenotype Manifest In

Enhancer of

Statement

Reference

Wnt4^UAS.cGa/Scer\GAL4^bi-omb-Gal4 is an enhancer of eye disc phenotype of Scer\GAL4^bi-omb-Gal4, fj^UAS.cZa

(Lim et al., 2005)

NOT Enhancer of

Statement

Reference

Scer\GAL4^C587/Wnt4^UAS.cGa is a non-enhancer of female germline cyst | increased number phenotype of Scer\GAL4^C587, dlp^UAS.cBa

(Waghmare et al., 2020)

Scer\GAL4^C587/Wnt4^UAS.cGa is a non-enhancer of female germ cell nucleus phenotype of Scer\GAL4^C587, dlp^UAS.cBa

(Waghmare et al., 2020)

Suppressor of

Statement

Reference

Scer\GAL4^C587/Wnt4^UAS.cGa is a suppressor | partially of spectrosome | increased number | oogenesis phenotype of Scer\GAL4^C587, dlp^UAS.cBa

(Waghmare et al., 2020)

Scer\GAL4^C587/Wnt4^UAS.cGa is a suppressor of escort cell | decreased number phenotype of Scer\GAL4^C587, dlp^UAS.cBa

(Waghmare et al., 2020)

Scer\GAL4^C587/Wnt4^UAS.cGa is a suppressor | partially of female germline stem cell | decreased number phenotype of Scer\GAL4^C587, dlp^UAS.cBa

(Waghmare et al., 2020)

Wnt4^UAS.cGa/Scer\GAL4^dpp.blk1 is a suppressor of wing hair phenotype of Scer\GAL4^dpp.blk1, fz^UAS.cAa

(Lim et al., 2005)

Scer\GAL4^ptc-559.1/Wnt4^UAS.cGa is a suppressor of wing phenotype of wg^l-16/wg^l-17

(Gieseler et al., 2001)

Scer\GAL4^ptc-559.1/Wnt4^UAS.cGa is a suppressor | partially of wing disc phenotype of wg^l-12/wg^l-17

(Gieseler et al., 2001)

Scer\GAL4^ptc-559.1/Wnt4^UAS.cGa is a suppressor | partially of antenna phenotype of wg^l-16/wg^l-17

(Gieseler et al., 2001)

Scer\GAL4^ptc-559.1/Wnt4^UAS.cGa is a suppressor of wing disc phenotype of wg^l-16/wg^l-17

(Gieseler et al., 2001)

Scer\GAL4^ptc-559.1/Wnt4^UAS.cGa is a suppressor of haltere phenotype of wg^l-16/wg^l-17

(Gieseler et al., 2001)

Scer\GAL4^ptc-559.1/Wnt4^UAS.cGa is a suppressor of leg phenotype of wg^l-16/wg^l-17

(Gieseler et al., 2001)

Scer\GAL4^ptc-559.1/Wnt4^UAS.cGa is a suppressor of mesonotum | ectopic phenotype of wg^l-16/wg^l-17

(Gieseler et al., 2001)

Wnt4^UAS.cGa/Scer\GAL4^da.G32 is a suppressor of embryonic epidermis | dorsal phenotype of hh⁴

(Buratovich et al., 2000)

Wnt4^UAS.cGa/Scer\GAL4^da.G32 is a suppressor of dorsal denticle phenotype of hh⁴

(Buratovich et al., 2000)

NOT Suppressor of

Statement

Reference

Scer\GAL4^C587/Wnt4^UAS.cGa is a non-suppressor of female germline cyst | increased number phenotype of Scer\GAL4^C587, dlp^UAS.cBa

(Waghmare et al., 2020)

Scer\GAL4^C587/Wnt4^UAS.cGa is a non-suppressor of female germ cell nucleus phenotype of Scer\GAL4^C587, dlp^UAS.cBa

(Waghmare et al., 2020)

Wnt4^UAS.cGa/Scer\GAL4^da.G32 is a non-suppressor of phenotype of wg^l-17

(Buratovich et al., 2000)

Other

Statement

Reference

Scer\GAL4^da.G32, Wnt4^UAS.cGa, otk^UAS.cWa has embryonic/first instar larval cuticle phenotype

(Peradziryi et al., 2011)

Scer\GAL4^da.G32, Wnt4^UAS.cGa, otk^UAS.cWa has denticle | ectopic phenotype

(Peradziryi et al., 2011)

Scer\GAL4^dpp.blk1, Wnt4^UAS.cGa, fj^UAS.cZa has wing hair phenotype

(Lim et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Wnt4^Scer\UAS.cGa and otk^Scer\UAS.cWa under the control of Scer\GAL4^da.G32 results in embryos where all epidermal cells secrete denticles.

Embryos expressing Wnt4^Scer\UAS.cGa under the control of Scer\GAL4^da.G32 in a otk³ background show loss of denticle rows similar to that see in otk³ single mutants.

(Peradziryi et al., 2011)

The weak polarity reversal phenotype caused by expression of fj^Scer\UAS.cZa under the control of Scer\GAL4^bi-omb-Gal4 is enhanced by co-expression of Wnt4^Scer\UAS.cGa.

Expression of either fj^Scer\UAS.cZa or Wnt4^Scer\UAS.cGa singly under the control of Scer\GAL4^dpp.blk1 at 20^oC does not result in strong polarity changes in the wing. However, co-expression of both fj^Scer\UAS.cZa and Wnt4^Scer\UAS.cGa under the control of Scer\GAL4^dpp.blk1 results in strong polarity reversals both in the distal (60% penetrance) and proximal (40% penetrance) regions of the wing.

Co-expression of Wnt4^Scer\UAS.cGa suppresses the change in wing hair polarity caused by expression of fz^Scer\UAS.cAa under the control of Scer\GAL4^dpp.blk1.

(Lim et al., 2005)

Many of the phenotypes caused by wg^l-16/wg^l-17 can be rescued by expression of Wnt4^Scer\UAS.cGa under the control of Scer\GAL4^ptc-559.1; wing rescue is seen at high frequency, halteres appear well developed, leg morphology is often rescued, but partial rescue of the antenna is only occasionally seen. The wing disc defect caused by shifting wg^l-12/wg^l-17 wing discs to the restrictive temperature for 24 hours at the beginning of the second larval instar can be rescued by expression of Wnt4^Scer\UAS.cGa under the control of Scer\GAL4^ptc-559.1 during the 24 hour period. When wg^l-12/wg^l-17 wing discs expressing Wnt4^Scer\UAS.cGa under the control of Scer\GAL4^ptc-559.1 are continuously maintained at the restrictive temperature from the second until the late third instar stage, the resulting discs fail to develop normally and remain very small.

(Gieseler et al., 2001)

Expression of Wnt4^Scer\UAS.cGa under the control of Scer\GAL4^da.G32 does not rescue the phenotype of wg^l-17 embryos. Loss of hh activity at 6 hours of development in hh⁴ homozygous embryos results in the loss of 1-3^o cell fates in the dorsal epidermis. This phenotype is not altered by the expression of Wnt4^Scer\UAS.cGa under the control of Scer\GAL4^da.G32. Loss of hh activity at 7 hours of development in hh⁴ homozygous embryos results in production of a few 3^o denticles in the dorsal epidermis. Expression of Wnt4^Scer\UAS.cGa under the control of Scer\GAL4^da.G32 in this background expands the production of 3^o denticles in each segment.

(Buratovich et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

80070

w^*; P{UAS-Wnt4.G}4-6-1, P{UAS-Wnt4.G}4-6-2; P{UAS-Wnt4.G}13

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Wnt4^Scer\UAS.cGa

Wnt4^UAS.cGa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Gieseler

(Gieseler et al., 1999)

Secondary FlyBase IDs

References (16)

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