FlyBase Allele Report: Dmel\Dronc[C318G.UAS]

General Information

Symbol

Dmel\Dronc^C318G.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0104748

Feature type

allele

Associated gene

Dmel\Dronc

Associated Insertion(s)

Carried in Construct

P{UAS-dronc.C318G}

Also Known As

uas-dronc^DN

Key Links

Transgenic product class

missense_variant

(Igaki et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Igaki et al., 2000)

Mutagen

in vitro construct

(Igaki et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-dronc.C318G}

Cytology

Description

UAS sequences drive an Dronc active-site mutant (carries the amino acid replacement C318G).

(Igaki et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Dronc

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^sca-P309

(Kanuka et al., 2005)

Phenotype Manifest In

eo neuron | increased number, with Scer\GAL4^sca-P309

(Kanuka et al., 2005)

macrochaeta | increased number, with Scer\GAL4^sca-P309

(Kanuka et al., 2005)

sensory mother cell | increased number, with Scer\GAL4^sca-P309

(Kanuka et al., 2005)

thecogen cell | increased number, with Scer\GAL4^sca-P309

(Kanuka et al., 2005)

Detailed Description

Statement

Reference

Ectopic macrochaetae form on the scutellum in 55.2% of cases when Nc^{C318G.Scer\UAS} is expressed under the control of Scer\GAL4^sca-P309. In contrast, the ectopic expression of Nc^{C318G.Scer\UAS} in SOP cells, driven by Scer\GAL4^{neur-GAL4-A101}, does not result in extra macrochaetae. Larvae expressing Nc^{C318G.Scer\UAS} under the control of Scer\GAL4^sca-P309 have one extra SOP cell in the scutellum region of the third instar wing disc. Analysis of the scutellum region of the pupal notum (24---30 h after pupal formation (APF)) shows that there is an extra neuron and thecogen present, which are derived from the extra SOP cell. No significant increase in macrochaetae number is seen when Nc^{C318G.Scer\UAS} is expressed under the control of Scer\GAL4^hs.PB following a heat shock at late third-instar (6-12 h APF), which is during SOP cell formation, or after it (24-30 h APF). At the stage prior to the appearance SOP cells, however, Nc^{C318G.Scer\UAS} expression leads to extra macrochaetae formation.

(Kanuka et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Dronc^C318G.UAS, Scer\GAL4^hh-Gal4 is a non-enhancer of abnormal size phenotype of Axud1^RNAi.UAS, Scer\GAL4^hh-Gal4

(Glavic et al., 2009)

Suppressor of

Statement

Reference

Dronc^C318G.UAS, Scer\GAL4^GMR.PF is a suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF, egr^UAS.cIa

(Ma et al., 2012)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}, Dronc^C318G.UAS is a non-suppressor of decreased cell number | embryonic stage phenotype of AIF^UAS.Tag:MYC, DNaseII^UAS.cTa, Scer\GAL4^{VP16.nanos.UTR}

(Tarayrah-Ibraheim et al., 2021)

Dronc^C318G.UAS, Scer\GAL4^hh-Gal4 is a non-suppressor of abnormal size phenotype of Axud1^RNAi.UAS, Scer\GAL4^hh-Gal4

(Glavic et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Dronc^C318G.UAS, Scer\GAL4^sca-P309 has macrochaeta | increased number phenotype, enhanceable by sgg³²

(Kanuka et al., 2005)

Suppressed by

Statement

Reference

Dronc^C318G.UAS, Scer\GAL4^sca-P309 has macrochaeta | increased number phenotype, suppressible by wg^Sp-1

(Kanuka et al., 2005)

NOT Enhancer of

Statement

Reference

Dronc^C318G.UAS, Scer\GAL4^hh-Gal4 is a non-enhancer of wing blade phenotype of Axud1^RNAi.UAS, Scer\GAL4^hh-Gal4

(Glavic et al., 2009)

Suppressor of

Statement

Reference

Dronc^C318G.UAS, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, egr^UAS.cIa

(Ma et al., 2012)

Dronc^C318G.UAS, Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of IKKε^UAS.cKa, Scer\GAL4^GMR.PF

(Kuranaga et al., 2006)

Dronc^C318G.UAS is a suppressor of wing disc | somatic clone phenotype of BacA\p35^UAS.cHa, Scer\GAL4^αTub84B.PZ, hid^UAS.cZa

(Ryoo et al., 2004)

Dronc^C318G.UAS, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, rpr^UAS.cIa

(Igaki et al., 2002)

Dronc^C318G.UAS, Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PF, egr^GS9830

(Igaki et al., 2002)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}, Dronc^C318G.UAS is a non-suppressor of primordial germ cell | embryonic stage | decreased number phenotype of AIF^UAS.Tag:MYC, DNaseII^UAS.cTa, Scer\GAL4^{VP16.nanos.UTR}

(Tarayrah-Ibraheim et al., 2021)

Dronc^C318G.UAS, Scer\GAL4^hh-Gal4 is a non-suppressor of wing blade phenotype of Axud1^RNAi.UAS, Scer\GAL4^hh-Gal4

(Glavic et al., 2009)

Dronc^C318G.UAS/Scer\GAL4^unspecified is a non-suppressor of phenotype of Debcl^GMR.PI

(Igaki et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Nc^{C318G.Scer\UAS} suppresses the small eye phenotype in flies expressing egr^Scer\UAS.cIa under the control of Scer\GAL4^GMR.PF.

(Ma et al., 2012)

The wing size reduction resulting from the Scer\GAL4^hh-Gal4-driven expression of Axud1^{dsRNA.Scer\UAS} is not affected by the co-expression of Nc^{C318G.Scer\UAS}.

(Glavic et al., 2009)

Expression of Nc^{C318G.Scer\UAS} partially suppresses the eye ablation phenotype seen in Scer\GAL4^GMR.PF>ik2^Scer\UAS.cKa flies.

(Kuranaga et al., 2006)

The wg^Sp-1 mutation is able to suppress the increase in extra macrochaetae seen when Nc^{C318G.Scer\UAS} is expressed under the control of Scer\GAL4^sca-P309, while the sgg³² null mutation enhances this phenotype.

(Kanuka et al., 2005)

The cell death seen in S2 cells treated with th dsRNA (produced by annealing th^cIa and th^a.cIa RNA) is enhanced if the cells are also coexpressing Nc^{C318G.Scer\UAS} and Ark^Scer\UAS.cIa under the control of Scer\GAL4^Act5C.PHb.

(Igaki et al., 2002)

Xenogenetic Interactions

Statement

Reference

W^Scer\UAS.cZa; BacA\p35^Scer\UAS.cHa; Scer\GAL4^αTub84B.PZ somatic clones in the wing disc cause significant overgrowth of this disc. This overgrowth in suppressed by Nc^{C318G.Scer\UAS}.

(Ryoo et al., 2004)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in plasmid "pUAS-mDRONC" and transfected into S2 cells to study its effect on debcl induced apoptosis.

(Igaki et al., 2000)

Carried in a plasmid and transfected into S2 cells.

(Igaki et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

DRONC^DN

(Zhang et al., 2015)

Dronc^{C318G.Scer\UAS}

Dronc^C318G.UAS

Nc^{C318G.Scer\UAS}

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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