FlyBase Allele Report: Dmel\Shc[BG]

General Information

Symbol

Dmel\Shc^BG

Species

D. melanogaster

Name

FlyBase ID

FBal0105104

Feature type

allele

Associated gene

Dmel\Shc

Associated Insertion(s)

Carried in Construct

Also Known As

dshc^BG

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Nucleotide substitution: C?A. Nucleotide substitution: A?T. Amino acid replacement: T297N. Above mutation followed by insertion of two C nucleotides, resulting in a frameshift at the N-terminal end of the SH2 domain and a subsequent stop codon at position 337.

(Luschnig et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

3L:9,428,094..9,428,096 [+]

Comment:

A two base substitution in Shc codon 297 and a two base insertion in codon 298 results in a net two base insertion, a frameshift, and early translation termination.

Inserted_sequence:

ATGCC

(Luschnig et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity | recessive

(Luschnig et al., 2000)

female sterile | recessive

(Luschnig et al., 2000)

lethal | embryonic stage | maternal effect | recessive

(Luschnig et al., 2000)

partially lethal - majority die | pupal stage | recessive

(Luschnig et al., 2000)

Phenotype Manifest In

cephalopharyngeal skeleton | germline clone | maternal effect

(Luschnig et al., 2000)

dorsal air sac primordium | somatic clone

(Cabernard and Affolter, 2005)

dorsal appendage | maternal effect

(Luschnig et al., 2000)

egg | maternal effect

(Luschnig et al., 2000)

egg chorion | maternal effect

(Luschnig et al., 2000)

embryo | extended germ band stage | germline clone | maternal effect

(Luschnig et al., 2000)

embryonic/first instar larval cuticle | germline clone | maternal effect

(Luschnig et al., 2000)

embryonic abdominal segment 8 | germline clone | maternal effect

(Luschnig et al., 2000)

eye | anterior | maternal effect

(Luschnig et al., 2000)

eye | maternal effect

(Luschnig et al., 2000)

eye photoreceptor cell | maternal effect

(Luschnig et al., 2000)

filzkorper | germline clone | maternal effect

(Luschnig et al., 2000)

Malpighian tubule | germline clone | maternal effect

(Luschnig et al., 2000)

ommatidium | maternal effect

(Luschnig et al., 2000)

ovary | maternal effect

(Luschnig et al., 2000)

ventral denticle belt | germline clone | maternal effect

(Luschnig et al., 2000)

wing | maternal effect

(Luschnig et al., 2000)

wing vein L4 | distal | maternal effect

(Luschnig et al., 2000)

Detailed Description

Statement

Reference

Shc^BG/Shc^BG mushroom body clones have normal axon pruning.

(Bornstein et al., 2015)

Shc^BG homozygous clones in the dorsal air sac primordium are found at the tip of the primordium at a significantly lower frequency than wild-type clones.

(Cabernard and Affolter, 2005)

Embryos derived from homozygous female germ-line clones display terminal defects. Most embryos fail to hatch and show a reduction of posterior Filzkorper and the 8th abdominal segment as well as mild defects in the anterior head skeleton. Malpighian tubules are always absent. Embryos lacking both maternal and zygotic Shc function show defects in germband retraction and a variable reduction of ventral denticle belts not seen in paternally rescued embryos. 70% of homozygotes die during the late pupal stage. Females that survive to adulthood do not lay eggs. Homozygous adult survivors are delayed in development and show defects in the eye, wing and ovary. The eyes are reduced in size and slightly roughened. The severity of the phenotype is graded in an anterior to posterior fashion, with the anterior side being more disorganised than the posterior. The ommatidia in the posterior region of the eye appear essentially normal and contain the full complement of photoreceptors, whereas in the anterior part of the eye, several ommatidia lack one or more outer photoreceptors. These mutant ommatidia do contain small central R7-like photoreceptors. In some ommatidia, the photoreceptors show rotated planar polarity. There is a partially penetrant loss of wing vein material in the distal part of the L4 vein. Homozygous females contain mature oocytes surrounded by a weakly ventralised chorion (as indicated by the fusion of the dorsal appendages).

(Luschnig et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Shc^BG/Shc[+] is a suppressor of visible | semidominant phenotype of Egfr^E3

(Luschnig et al., 2000)

Shc^BG/Shc^BG is a suppressor of visible phenotype of Scer\GAL4^CY2, btl::Egfr^λ.UAS

(Luschnig et al., 2000)

NOT Suppressor of

Statement

Reference

Shc^BG is a non-suppressor of visible phenotype of sev^S11.Tag:MYC

(Luschnig et al., 2000)

Other

Statement

Reference

Egfr^E3, Shc^BG has viable phenotype

(Luschnig et al., 2000)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Shc^BG is a non-enhancer of phenotype of Ras85D^V12.sev

(Luschnig et al., 2000)

Shc^BG is a non-enhancer of phenotype of rl^Sem

(Luschnig et al., 2000)

Shc^BG is a non-enhancer of phenotype of Raf::tor^12D.hs.sev

(Luschnig et al., 2000)

Suppressor of

Statement

Reference

Shc^BG/Shc[+] is a suppressor of eye phenotype of Egfr^E3

(Luschnig et al., 2000)

Shc^BG/Shc[+] is a suppressor of wing phenotype of Egfr^E3

(Luschnig et al., 2000)

Shc^BG is a suppressor of abdominal 11 anal tuft phenotype of tor^13D

(Luschnig et al., 2000)

Shc^BG is a suppressor of embryonic/first instar larval cuticle phenotype of tor^13D

(Luschnig et al., 2000)

Shc^BG is a suppressor of filzkorper phenotype of tor^13D

(Luschnig et al., 2000)

Shc^BG/Shc^BG is a suppressor | partially of dorsal appendage phenotype of Scer\GAL4^CY2, btl::Egfr^λ.UAS

(Luschnig et al., 2000)

Shc^BG/Shc^BG is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^CY2, btl::Egfr^λ.UAS

(Luschnig et al., 2000)

Shc^BG/Shc^BG is a suppressor of wing phenotype of Scer\GAL4^CY2, btl::Egfr^λ.UAS

(Luschnig et al., 2000)

NOT Suppressor of

Statement

Reference

Shc^BG is a non-suppressor of phenotype of Ras85D^V12.sev

(Luschnig et al., 2000)

Shc^BG is a non-suppressor of phenotype of Raf::tor^12D.hs.sev

(Luschnig et al., 2000)

Shc^BG is a non-suppressor of phenotype of rl^Sem

(Luschnig et al., 2000)

Shc^BG is a non-suppressor of eye phenotype of sev^S11.Tag:MYC

(Luschnig et al., 2000)

Shc^BG is a non-suppressor of ommatidium phenotype of sev^S11.Tag:MYC

(Luschnig et al., 2000)

Other

Statement

Reference

Shc^BG, dos^P115 has cephalopharyngeal skeleton | germline clone phenotype

(Luschnig et al., 2000)

Shc^BG, dos^P115 has embryonic abdominal segment 8 | germline clone phenotype

(Luschnig et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygous females carrying tor^13D produce embryos that produce very little cuticle. This phenotype is weakly suppressed if the females are carrying one copy of Shc^BG; the embryos develop more cuticle and show posterior structures such as Filzkorper and Tuft structures. The phenotype is completely suppressed if the tor^13D/+ females also carry homozygous Shc^BG germline clones; embryos look normal and can hatch. The eye and wing phenotypes caused by Egfr^E3 are dominantly suppressed by Shc^BG. The Egfr^E3 phenotype is completely suppressed if the flies are also homozygous for Shc^BG and the viability of these flies is restored. Expression of btl::Egfr^{Scer\UAS.T:λ\cI-DD} under the control of Scer\GAL4^CY2 results in the formation of ectopic wing vein material at multiple sites on the wing. This phenotype is completely suppressed if the flies are also homozygous for Shc^BG. Females expressing btl::Egfr^{Scer\UAS.T:λ\cI-DD} under the control of Scer\GAL4^CY2 produce strongly dorsalised eggs with no dorsal appendages. This phenotype is partially suppressed if the females are also homozygous for Shc^BG; dorsal appendage material is produced around the anterior circumference of the egg. 85% of embryos derived from dos^P115 Shc^BG double mutant female germline clones lack all structures posterior to segment A7 and have reduced head skeletons. The remaining 15% retain segment A8 but no structures posterior to it. Embryos derived from Shc^BG drk^ΔP24 double mutant female germline clones have a stronger phenotype than embryos derived from either single mutant female germline clone.

(Luschnig et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a dominant enhancer of the haploinsufficient dl mutant phenotype.

(Luschnig et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Shc^BG

(Bornstein et al., 2015)

dshc^BG

(Cabernard and Affolter, 2005, Settle et al., 2003, Luschnig et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (4)

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