FlyBase Allele Report: Dmel\ush[UAS.cFa]

General Information

Symbol

Dmel\ush^UAS.cFa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Fossett

FlyBase ID

FBal0117270

Feature type

allele

Associated gene

Dmel\ush

Associated Insertion(s)

Carried in Construct

P{UAS-ush.F}

Also Known As

UAS-ush

Key Links

Transgenic product class

cDNA

(Fossett et al., 2000)

wild_type

(Fossett et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Fossett et al., 2000)

wild_type

(Fossett et al., 2000)

Mutagen

in vitro construct

(Fossett et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-ush.F}

Cytology

Description

A full length ush cDNA is expressed under the control of UASt sequences.

(Fossett et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

ush

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity, with Scer\GAL4^ey.PH

(Fossett et al., 2001)

abnormal size | adult stage

(Kennell et al., 2012)

decreased cell number | third instar larval stage, with Scer\GAL4^Cg.PA

(Gao et al., 2009)

partially lethal - majority die, with Scer\GAL4^ey.PH

(Fossett et al., 2001)

some die during pupal stage, with Scer\GAL4^ey.PH

(Fossett et al., 2001)

viable, with Scer\GAL4^twi.PB

(Muratoglu et al., 2006)

visible, with Scer\GAL4^ey.PH

(Fossett et al., 2001)

visible | adult stage

(Kennell et al., 2012)

visible | heat sensitive, with Scer\GAL4^ey.PH

(Singh and Choi, 2003)

Phenotype Manifest In

antenna, with Scer\GAL4^ey.PH

(Singh and Choi, 2003)

antenna | ectopic, with Scer\GAL4^ey.PH

(Fossett et al., 2001)

dorsal vessel wall cell, with Scer\GAL4^twi.PB

(Fossett et al., 2000)

embryonic/larval crystal cell, with Scer\GAL4^lz-gal4

(Fossett et al., 2001)

embryonic/larval crystal cell, with Scer\GAL4^twi.PB

(Fossett et al., 2003)

embryonic/larval lymph gland, with Scer\GAL4^Mef2.PR

(Muratoglu et al., 2006)

embryonic/larval lymph gland, with Scer\GAL4^twi.PB

(Muratoglu et al., 2006)

embryonic/larval plasmatocyte | third instar larval stage, with Scer\GAL4^Cg.PA

(Gao et al., 2009)

eye, with Scer\GAL4^ey.PH

(Singh and Choi, 2003, Fossett et al., 2001)

eye disc | heat sensitive, with Scer\GAL4^ey.PH

(Singh et al., 2005)

heart primordium, with Scer\GAL4^twi.PB

(Fossett et al., 2001)

ommatidium, with Scer\GAL4^ey.PH

(Fossett et al., 2001)

posterior signaling center of lymph gland primary lobe, with Scer\GAL4^{kn-col85-GAL4}

(Tokusumi et al., 2010)

Detailed Description

Statement

Reference

Expression of ush^Scer\UAS.cFa at 27[o]C in the fat body using Scer\GAL4^Cg.PA results in flies that are proportionally smaller than control flies. No difference is seen in the pigmentation of abdominal segment A6.

(Kennell et al., 2012)

Expression of ush^Scer\UAS.cFa in the posterior signaling center under the control of Scer\GAL4^{kn-col85-GAL4} leads to defects in niche cell differentiation based on a lack of filopodia formation.

(Tokusumi et al., 2010)

Expression of ush^Scer\UAS.cFa under the control of Scer\GAL4^Cg.PA results in an almost complete lost of plasmatocytes in third instar larval lymph glands. Lymph gland size is normal in these animals.

(Gao et al., 2009)

Expression of ush^Scer\UAS.cFa, under the control of either Scer\GAL4^twi.PB or Scer\GAL4^Mef2.PR, results in impaired lymph gland development.

(Muratoglu et al., 2006)

Expression of ush^Scer\UAS.cFa under the control of Scer\GAL4^ey.PH at 29^oC throughout development results in a very small eye field in the eye-antennal disc (in 80% of discs no eye field is seen), whereas the antennal field is relatively normal.

(Singh et al., 2005)

When ush^Scer\UAS.cFa is driven by Scer\GAL4^twi.PB a reduced number of crystal cells is seen in the mutant embryo.

(Fossett et al., 2003)

Expression of ush^Scer\UAS.cFa under the control of Scer\GAL4^ey.PH at 29^oC results in adults that either lack eyes or have a small eye phenotype. Antennal development is also affected. Expression of ush^Scer\UAS.cFa under the control of Scer\GAL4^ey.PH in animals that are maintained at 18^oC throughout embryogenesis and are then shifted to 29^oC results in elimination of the eye field without affecting antennal development. Expression of ush^Scer\UAS.cFa under the control of Scer\GAL4^ey.PH in animals that are maintained at 29^oC throughout embryogenesis and are then shifted to 18^oC has a very subtle effect or no effect on eye development. Expression of ush^Scer\UAS.cFa under the control of Scer\GAL4^ey.PH in animals that are shifted 29^oC during the early first larval instar and are then shifted back to 18^oC results in normal eye development.

(Singh and Choi, 2003)

When ush^Scer\UAS.cFa is driven by Scer\GAL4^lz-gal4 a significant decrease in the average number of crystal cells is seen, 30% at stage 13 embryos and 85% at stage 16 embryos. When ush^Scer\UAS.cFa is driven by Scer\GAL4^twi.PB a reduction in the umber of cardiac cells is seen in embryos. An average of 40% of embryos have gaps in the heart tube ranging from 10 cells missing to a complete absence of cardiac cells. When ush^Scer\UAS.cFa is driven by Scer\GAL4^ey.PH severe defects are seen in the eye. These include ectopic production of antennae, reduction or loss of the eye, disrupted ommatidial polarity. This combination is also lethal for about 80% of the population before eclosion.

(Fossett et al., 2001)

Expression of ush^Scer\UAS.cFa driven by Scer\GAL4^twi.PB leads to a significant reduction in the number of cardial cells. Up to about 50% of cells are missing, with small gaps or large deletions present in the heart tubes.

(Fossett et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

ush^UAS.cFa/Scer\GAL4^ey.PH is a suppressor of visible | dominant phenotype of L²

(Singh et al., 2005)

Other

Statement

Reference

L², Scer\GAL4^ey.PH, ush^UAS.cFa has lethal phenotype

(Singh et al., 2005)

L²/L[+], Scer\GAL4^ey.PH, ush^UAS.cFa has lethal phenotype

(Singh et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^twi.PB, ush^UAS.cFa has embryonic/larval crystal cell phenotype, enhanceable by srp^NC.F.UAS/Scer\GAL4^twi.PB

(Fossett et al., 2003)

NOT Enhanced by

Statement

Reference

Scer\GAL4^twi.PB, ush^UAS.cFa has embryonic/larval crystal cell phenotype, non-enhanceable by srp^C.UAS.cHa/Scer\GAL4^twi.PB

(Fossett et al., 2003)

Scer\GAL4^twi.PB, ush^UAS.cFa has embryonic/larval crystal cell phenotype, non-enhanceable by srp^NC.V421G.UAS/Scer\GAL4^twi.PB

(Fossett et al., 2003)

NOT suppressed by

Statement

Reference

Scer\GAL4^twi.PB, ush^UAS.cFa has embryonic/larval crystal cell phenotype, non-suppressible by srp^C.UAS.cHa/Scer\GAL4^twi.PB

(Fossett et al., 2003)

Scer\GAL4^twi.PB, ush^UAS.cFa has embryonic/larval crystal cell phenotype, non-suppressible by srp^NC.V421G.UAS/Scer\GAL4^twi.PB

(Fossett et al., 2003)

Suppressor of

Statement

Reference

Scer\GAL4^Cg.PA/ush^UAS.cFa is a suppressor of lamellocyte phenotype of hop^Tum

(Sorrentino et al., 2007)

ush^UAS.cFa/Scer\GAL4^ey.PH is a suppressor of eye | ventral phenotype of L²

(Singh et al., 2005)

Scer\GAL4^twi.PB/ush^UAS.cFa is a suppressor of embryonic/larval crystal cell | ectopic phenotype of Scer\GAL4^twi.PB, srp^NC.F.UAS

(Fossett et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Lymph glands in hop^Tum/Y animals expressing ush^Scer\UAS.cFa under the control of Scer\GAL4^Cg.PA are hypertrophic and produce lamellocytes, although the number of circulating lamellocytes is reduced by 90%.

(Sorrentino et al., 2007)

L²/+ increases the frequency of the no-eye phenotype seen in discs of animals expressing ush^Scer\UAS.cFa under the control of Scer\GAL4^ey.PH at 29^oC throughout development from 80% to nearly 98% and the animals fail to eclose. The L²/+ phenotype of ventral eye loss is unaffected if expression of ush^Scer\UAS.cFa is driven by Scer\GAL4^ey.PH at 29^oC from embryogenesis until the end of the first larval instar, or by Scer\GAL4^ey.PH at 29^oC from during the third larval instar. However, if ush^Scer\UAS.cFa expression is driven by Scer\GAL4^ey.PH at 29^oC during the second larval instar, the L²/+ ventral eye phenotype is significantly rescued.

(Singh et al., 2005)

The addition of srp^{NC.F.Scer\UAS} to ush^Scer\UAS.cFa, Scer\GAL4^twi.PB embryos enhances the loss of crystal phenotype seen in these animals. The addition of srp^{C.Scer\UAS.cHa} or srp^{NC.V421G.Scer\UAS} has no effect on the crystal cell phenotype.

(Fossett et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ush^Scer\UAS.cFa

ush^UAS.cFa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Fossett

(Fossett et al., 2000)

Secondary FlyBase IDs

References (16)

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