FlyBase Allele Report: Dmel\WASp[1]

General Information

Symbol

Dmel\WASp¹

Species

D. melanogaster

Name

FlyBase ID

FBal0121875

Feature type

allele

Associated gene

Dmel\WASp

Associated Insertion(s)

Carried in Construct

Also Known As

wsp¹

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

A 34 nucleotide deletion between positions 24652161-24652128 on arm 3R of genome sequence release 4.3. Missing sequence is: aacgacgaagtgctgaacgagttcttcgtgaagg. This deletion results in a frameshift after residue Glu-260 of the protein and termination after 8 additional residues.

(Schejter, 2006.7.3)

Small intragenic deletion resulting in a predicted frameshift which causes the cytoskeleton-interacting carboxy-terminal domain to be lost.

(Ben-Yaacov et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:28,826,406..28,826,439 [-]

Comment:

WASp[1]- A 34 nt deletion within WASp. The missing sequence is: aacgacgaagtgctgaacgagttcttcgtgaagg. The deletion results in a frameshift after residue Glu-260 of the protein and early translation termination after the addition of 7 novel amino acids.

(Schejter, 2006.7.3)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | recessive

(Ben-Yaacov et al., 2001)

abnormal neuroanatomy

(Coyle et al., 2004)

abnormal neuroanatomy | pupal stage (with Df(3R)3450)

(Brinkmann et al., 2016)

abnormal neuroanatomy | third instar larval stage (with Df(3R)3450)

(Nahm et al., 2010)

decreased rate of movement | recessive

(Ben-Yaacov et al., 2001)

decreased size | oogenesis

(Stark et al., 2021)

increased cell number | pupal stage (with Df(3R)3450)

(Brinkmann et al., 2016)

lethal (with Df(3R)3450)

(Tal et al., 2002)

lethal | recessive

(Coyle et al., 2004)

lethal - all die before end of P-stage (with WASp³)

(Zelhof and Hardy, 2004)

lethal - all die before end of P-stage | recessive

(Ben-Yaacov et al., 2001)

male sterile (with Df(3R)3450), with Scer\GAL4^arm.PS, WASp^H242D.UAS

(Rotkopf et al., 2011)

partially lethal

(Brinkmann et al., 2016)

partially lethal (with Df(3R)3450)

(Brinkmann et al., 2016)

some die during pharate adult stage (with WASp³)

(Zelhof and Hardy, 2004)

some die during pharate adult stage | recessive

(Ben-Yaacov et al., 2001)

Phenotype Manifest In

actin filament (with Df(3R)3450), with Scer\GAL4^arm.PS, WASp^H242D.UAS

(Rotkopf et al., 2011)

adult abdominal sensillum (with Df(3R)3450)

(Ben-Yaacov et al., 2001)

adult head bristle (with Df(3R)3450)

(Ben-Yaacov et al., 2001)

adult thorax & microchaeta (with Df(3R)3450)

(Ben-Yaacov et al., 2001)

bouton

(Coyle et al., 2004)

bouton | increased number

(Coyle et al., 2004)

dorsal acute muscle cell | embryonic stage

(Massarwa et al., 2007)

embryonic/larval neuromuscular junction | third instar larval stage (with Df(3R)3450)

(Khuong et al., 2010)

embryonic myoblast (with WASp^3D3-035)

(Schafer et al., 2007)

external sensory organ precursor cell | pupal stage (with Df(3R)3450)

(Brinkmann et al., 2016)

head cyst cell (with Df(3R)3450), with Scer\GAL4^arm.PS, WASp^H242D.UAS

(Rotkopf et al., 2011)

interommatidial bristle (with Df(3R)3450)

(Ben-Yaacov et al., 2001)

interommatidial bristle & eo neuron | supernumerary (with Df(3R)3450)

(Ben-Yaacov et al., 2001)

interommatidial bristle & tormogen cell (with Df(3R)3450)

(Ben-Yaacov et al., 2001)

interommatidial bristle & trichogen cell (with Df(3R)3450)

(Ben-Yaacov et al., 2001)

interommatidial bristle | adult stage | somatic clone (with Df(3R)3450)

(Galy et al., 2011)

interommatidial bristle | somatic clone

(Zallen et al., 2002)

larval somatic muscle cell

(Massarwa et al., 2007)

lateral transverse muscle cell | late embryonic stage

(Brinkmann et al., 2016)

leg & microchaeta (with Df(3R)3450)

(Ben-Yaacov et al., 2001)

macrochaeta (with Df(3R)3450)

(Ben-Yaacov et al., 2001)

macrochaeta & adult head | somatic clone

(Zallen et al., 2002)

macrochaeta | immature adult stage

(Brinkmann et al., 2016)

macrochaeta | immature adult stage (with Df(3R)3450)

(Brinkmann et al., 2016)

macrochaeta | increased number (with Df(3R)3450)

(Ben-Yaacov et al., 2001)

microchaeta & adult head | somatic clone

(Zallen et al., 2002)

myoblast | embryonic stage

(Massarwa et al., 2007)

neuromuscular junction | larval stage

(Coyle et al., 2004)

NMJ bouton | third instar larval stage (with Df(3R)3450)

(Nahm et al., 2010)

nurse cell ring canal

(Stark et al., 2021)

primordial germ cell | embryonic stage

(Kim et al., 2021)

primordial germ cell | embryonic stage (with WASp³)

(Kim et al., 2021)

rhabdomere (with WASp³)

(Zelhof and Hardy, 2004)

rhabdomere | adult stage | somatic clone (with Df(3R)3450)

(Galy et al., 2011)

seminal vesicle (with Df(3R)3450), with Scer\GAL4^arm.PS, WASp^H242D.UAS

(Rotkopf et al., 2011)

sensory mother cell (with Df(3R)3450)

(Bogdan et al., 2005)

spermatid cyst (with Df(3R)3450), with Scer\GAL4^arm.PS, WASp^H242D.UAS

(Rotkopf et al., 2011)

synaptic vesicle | third instar larval stage (with Df(3R)3450)

(Khuong et al., 2010)

tormogen cell (with Df(3R)3450)

(Ben-Yaacov et al., 2001)

trichogen cell (with Df(3R)3450)

(Ben-Yaacov et al., 2001)

Detailed Description

Statement

Reference

Bouton numbers are unaffected at the NMJ of WASp¹/+ third instar larvae.

(Kim et al., 2019)

Isolated pupal macrophages (plated on cover slips) deficient of WASp¹/Df(3R)3450 do not show defects in hemocyte size.

WASp¹/Df(3R)3450 or WASp¹/WASp¹ survive until early adulthood, though many flies fail to fully eclose from the pupal case.

WASp¹/Df(3R)3450 newly eclosed flies have significantly fewer macrochaetes on the head and thorax compared to wild type. WASp¹/Df(3R)3450 pupal (24h APF) notae have a significantly increased number of neurons per sensory organ.

WASp¹/WASp¹ newly eclosed flies have significantly fewer macrochaetes on the head compared to wild type and late stage embryos show attachment defects of lateral transverse muscles.

(Brinkmann et al., 2016)

Mutant larvae do not show dorsal closure defects.

(Giuliani et al., 2013)

WASp¹/Df(3R)3450 do not show a kinked microchaete phenotype.

(Koch et al., 2012)

Minor shape abnormalities are seen in the rhabdomeres of WASp¹/Df(3R)3450 mutant adult eye clones. However rhabdomere extension occurs normally and the underlying fenestrated membrane is intact. Retina depth and external morphology are also normal. Many of the interommatidial bristles are missing.

(Galy et al., 2011)

As in wild type, flies expressing WASp^Scer\UAS.cBa under the control of Scer\GAL4^arm.PS in a WASp¹/Df(3R)3450 mutant background display elongated cysts and a seminal vesicle that is full of individualised, mature sperm.

Flies expressing WASp^{H242D.Scer\UAS} under the control of Scer\GAL4^arm.PS in a WASp¹/Df(3R)3450 mutant background display similarly elongated cysts to wild type, but no sperm is released into the seminal vesicle. Coiling of the cysts does not take place, and the extreme basal end of the testis tube, lined by the terminal epithelium and leading up to the seminal vesicle, is free of cysts altogether. Sperm individualisation in these mutants proceeds normally. The microfilaments and nuclei of late-stage cysts show significant structural alterations. There is a consistent failure to achieve the intertwined and tightly packed organisation of head cyst cell F-actin arrays and associated spermatid nuclei. The microfilament arrays appear sparse and misshapen, and the normally tight and uniformly oriented bundles of nuclei are loosely packed and partially split.

(Rotkopf et al., 2011)

WASp¹/Df(3R)3450 third instar larvae exhibit neuromuscular junction overgrowth.

WASp¹/Df(3R)3450 third instar larvae exhibit reduced vesicle cycling (reduced uptake of FM 1-43 dye) compared to controls.

(Khuong et al., 2010)

Third instar WASp¹/Df(3R)3450 larvae show an increase in bouton number/muscle area and satellite bouton number at the neuromuscular junction compared to wild type.

(Nahm et al., 2010)

WASp¹ heterozygous neuromuscular junctions do not exhibit any morphological changes.

(Nahm et al., 2010)

Muscle pattern in WASp¹ maternal/zygotic embryos are severely disrupted. A prominent feature is groups of mononucleated myoblasts clustered around thin, abnormally elongated fibers, suggesting WASp is required for myoblast fusion during embryonic myogenesis.

WASp¹ maternal/zygotic embryos display 2-3 DA1 nuclei per segment, implying that fusion is arrested after a single round of founder cell-fusion-competent myoblasts, generating a bi-/trinucleated myotube precursor.

(Massarwa et al., 2007)

WASp¹ homozygous embryos do not show a myoblast fusion phenotype.

WASp^3D3-035/WASp¹ transheterozygotes exhibit a myoblast fusion phenotype.

(Schafer et al., 2007)

WASp¹/Df(3R)3450 mutants loss of sensory organ precursor cells in the wing imaginal disc.

(Bogdan et al., 2005)

Occasional escapers survive to the pharate adult stage. Homozygous and hemizygous larvae show defects at the neuromuscular junction; bouton number, branch number, total NMJ length and hyperbranching of boutons are all increased compared to wild type. Heterozygotes show a low incidence of hyperbranching of boutons at the larval NMJ.

(Coyle et al., 2004)

WASp¹/WASp³ transheterozygotes survive to pharate adults. In these flies, many rhabdomeres do not obtain the normal characteristic oval shape, but rather are pointed and squared, with 2-3% of the mutant rhabdomeres split.

(Zelhof and Hardy, 2004)

In WASp¹/Df(3R)3450 mutants, interommatidial bristles are missing, along with nearly all bristles present on the dorsal aspect of the head in wild-type. However the ommatidial array is normal in these eyes.

(Zallen et al., 2002)

Hemizygotes complete nearly all stages of imaginal development and die as young adults, most commonly failing to eclose from the pupal case. Those that eclose can survive for a few days, but are lethargic and passive in their behaviour. WASp¹/Df(3R)3450 flies show a pronounced lack of neurosensory bristles, particularly on the head capsule and abdomen. Significant, but less severe effects are also seen on the legs and thorax, where microchaetae are primarily affected. Loss of both the bristle shaft and bristle socket is seen (smooth cuticle phenotype) and occasionally, bristles are duplicated. Bristles that do form have normal morphology. Strong loss of interommatidial bristles is seen in WASp¹/Df(3R)3450 flies, although the ordered spatial pattern of the eye facets is normal. Mutant pupae at 30 hours after puparium formation show an excess of neurons and a near absence of bristle shaft, socket and sheath cells in the developing retina.

(Ben-Yaacov et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Df(3R)3450/WASp¹ has increased cell number | pupal stage phenotype, enhanceable by Whamy^Δ109/Whamy[+]

(Brinkmann et al., 2016)

Df(3R)3450/WASp¹ has abnormal neuroanatomy | pupal stage phenotype, enhanceable by Whamy^Δ109/Whamy[+]

(Brinkmann et al., 2016)

WASp¹ has abnormal neuroanatomy phenotype, enhanceable by Cdc42[+]/Cip4¹/Cip4[+]/Cdc42²

(Nahm et al., 2010)

WASp¹ has abnormal neuroanatomy phenotype, enhanceable by nwk²

(Coyle et al., 2004)

Suppressed by

Statement

Reference

Df(3R)3450/WASp¹ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by RhoGAP92B¹/RhoGAP92B²

(Nahm et al., 2010)

Enhancer of

Statement

Reference

WASp[+]/WASp¹ is an enhancer of abnormal neuroanatomy phenotype of Cip4¹

(Nahm et al., 2010)

Cdc42[+], WASp[+], WASp¹, Cdc42² is an enhancer of abnormal neuroanatomy phenotype of Cip4¹

(Nahm et al., 2010)

Cip4¹, WASp[+], WASp¹, Cip4[+] is an enhancer of abnormal neuroanatomy phenotype of Cdc42²

(Nahm et al., 2010)

WASp¹ is an enhancer of abnormal neuroanatomy phenotype of nwk²

(Coyle et al., 2004)

Suppressor of

Statement

Reference

WASp[+]/WASp¹ is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of RhoGAP92B¹/RhoGAP92B²

(Nahm et al., 2010)

Other

Statement

Reference

Df(3R)3450/WASp¹, Whamy^Δ109/Whamy[+] has partially lethal phenotype

(Brinkmann et al., 2016)

WASp¹, Whamy^Δ109 has partially lethal phenotype

(Brinkmann et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

WASp³/WASp¹ has primordial germ cell | embryonic stage phenotype, enhanceable by PIP5K59B¹⁸

(Kim et al., 2021)

Df(3R)3450/WASp¹ has macrochaeta | immature adult stage phenotype, enhanceable by Whamy^Δ109/Whamy[+]

(Brinkmann et al., 2016)

Df(3R)3450/WASp¹ has external sensory organ precursor cell | pupal stage phenotype, enhanceable by Whamy^Δ109/Whamy[+]

(Brinkmann et al., 2016)

Df(3R)3450/WASp¹ has embryonic/larval neuromuscular junction | third instar larval stage phenotype, enhanceable by nwk¹

(Khuong et al., 2010)

WASp¹ has NMJ bouton phenotype, enhanceable by Cip4¹/Cip4[+]

(Nahm et al., 2010)

WASp¹ has neuromuscular junction phenotype, enhanceable by Cip4¹/Cip4[+]

(Nahm et al., 2010)

WASp¹ has NMJ bouton phenotype, enhanceable by Cdc42[+]/Cip4¹/Cip4[+]/Cdc42²

(Nahm et al., 2010)

WASp¹ has neuromuscular junction phenotype, enhanceable by Cdc42[+]/Cip4¹/Cip4[+]/Cdc42²

(Nahm et al., 2010)

WASp¹ has bouton | increased number phenotype, enhanceable by nwk²

(Coyle et al., 2004)

WASp¹ has bouton phenotype, enhanceable by nwk²

(Coyle et al., 2004)

WASp¹ has neuromuscular junction | larval stage phenotype, enhanceable by nwk²

(Coyle et al., 2004)

Df(3R)3450/WASp¹ has adult thorax & microchaeta phenotype, enhanceable by N^l1N-ts1

(Ben-Yaacov et al., 2001)

NOT Enhanced by

Statement

Reference

Df(3R)3450/WASp¹ has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-enhanceable by Scer\GAL4^nSyb.PS/tkv^{Q253D.UAS.cNb}

(Khuong et al., 2010)

Df(3R)3450/WASp¹ has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-enhanceable by Scer\GAL4^nSyb.PS/Avic\GFP^{EGFP.PLCD1(PH-S39R).UAS}

(Khuong et al., 2010)

Df(3R)3450/WASp¹ has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-enhanceable by Scer\GAL4^nSyb.PS/Avic\GFP^{EGFP.UAS.Tag:PH(hPLCD1)}

(Khuong et al., 2010)

Suppressed by

Statement

Reference

WASp¹ has macrochaeta | immature adult stage phenotype, suppressible by Whamy^UAS.EGFP/Scer\GAL4^sca.PU

(Brinkmann et al., 2016)

Df(3R)3450/WASp¹ has NMJ bouton | third instar larval stage phenotype, suppressible | partially by RhoGAP92B¹/RhoGAP92B²

(Nahm et al., 2010)

Df(3R)3450/WASp¹ has adult abdominal sensillum phenotype, suppressible by N^int.hs

(Ben-Yaacov et al., 2001)

Df(3R)3450/WASp¹ has interommatidial bristle phenotype, suppressible by H³

(Ben-Yaacov et al., 2001)

Enhancer of

Statement

Reference

Df(3R)3450/WASp¹ is an enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of nwk¹

(Khuong et al., 2010)

WASp[+]/WASp¹ is an enhancer of neuromuscular junction phenotype of Cip4¹

(Nahm et al., 2010)

Cdc42[+], WASp[+], WASp¹, Cdc42² is an enhancer of NMJ bouton phenotype of Cip4¹

(Nahm et al., 2010)

Cdc42[+], WASp[+], WASp¹, Cdc42² is an enhancer of neuromuscular junction phenotype of Cip4¹

(Nahm et al., 2010)

Cip4¹, WASp[+], WASp¹, Cip4[+] is an enhancer of NMJ bouton phenotype of Cdc42²

(Nahm et al., 2010)

Cip4¹, WASp[+], WASp¹, Cip4[+] is an enhancer of neuromuscular junction phenotype of Cdc42²

(Nahm et al., 2010)

WASp[+]/WASp¹ is an enhancer of NMJ bouton phenotype of Cip4¹

(Nahm et al., 2010)

WASp¹ is an enhancer of bouton | increased number phenotype of nwk²

(Coyle et al., 2004)

WASp¹ is an enhancer of bouton phenotype of nwk²

(Coyle et al., 2004)

WASp¹ is an enhancer of neuromuscular junction | larval stage phenotype of nwk²

(Coyle et al., 2004)

NOT Enhancer of

Statement

Reference

msn¹⁷², msn[+], WASp[+], WASp¹ is a non-enhancer of nurse cell ring canal phenotype of Scer\GAL4^{VP16.mat.αTub67C}, dock^{UASp.Tag:HA,Tag:Myr(Unk)}

(Stark et al., 2021)

Df(3R)3450/WASp¹ is a non-enhancer of embryonic/larval neuromuscular junction | third instar larval stage | somatic clone phenotype of tweek²/tweek¹

(Khuong et al., 2010)

Df(3R)3450/WASp¹ is a non-enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Avic\GFP^{EGFP.UAS.Tag:PH(hPLCD1)}, Scer\GAL4^nSyb.PS, nwk¹

(Khuong et al., 2010)

Suppressor of

Statement

Reference

WASp[+]/WASp¹ is a suppressor | partially of nurse cell ring canal phenotype of Scer\GAL4^{VP16.mat.αTub67C}, dock^{UASp.Tag:HA,Tag:Myr(Unk)}

(Stark et al., 2021)

Df(3R)3450/WASp¹ is a suppressor | partially of embryonic/larval neuromuscular junction | third instar larval stage phenotype of wit^B11/wit^A12

(Khuong et al., 2010)

WASp[+]/WASp¹ is a suppressor | partially of NMJ bouton | third instar larval stage phenotype of RhoGAP92B¹/RhoGAP92B²

(Nahm et al., 2010)

NOT Suppressor of

Statement

Reference

msn¹⁷², msn[+], WASp[+], WASp¹ is a non-suppressor of nurse cell ring canal phenotype of Scer\GAL4^{VP16.mat.αTub67C}, dock^{UASp.Tag:HA,Tag:Myr(Unk)}

(Stark et al., 2021)

WASp[+]/WASp¹ is a non-suppressor of microchaeta phenotype of Abp1^{UAS.Tag:Myr(Src64B),GFP}, Scer\GAL4^sca.PU

(Koch et al., 2012)

Other

Statement

Reference

WASp¹, Whamy^Δ109 has fusion competent cell | embryonic stage phenotype

(Brinkmann et al., 2016)

WASp¹, Whamy^Δ109 has larval somatic muscle cell | late embryonic stage phenotype

(Brinkmann et al., 2016)

WASp¹, Whamy^Δ109 has segment border muscle cell | late embryonic stage phenotype

(Brinkmann et al., 2016)

SCAR^k13811, WASp[+]/WASp¹ has larval longitudinal connective phenotype

(Zallen et al., 2002)

SCAR^k13811, WASp[+]/WASp¹ has larval ventral nerve cord phenotype

(Zallen et al., 2002)

Df(3R)3450/WASp¹, SCAR[+]/SCAR^k13811 has larval longitudinal connective phenotype

(Zallen et al., 2002)

Df(3R)3450/WASp¹, SCAR[+]/SCAR^k13811 has larval ventral nerve cord phenotype

(Zallen et al., 2002)

Df(3R)3450/WASp¹, SCAR[+]/SCAR^k13811 has larval ventral nerve cord commissure phenotype

(Zallen et al., 2002)

SCAR^k13811, WASp[+]/WASp¹ has larval ventral nerve cord commissure phenotype

(Zallen et al., 2002)

SCAR^k13811, WASp¹ has larval longitudinal connective phenotype

(Zallen et al., 2002)

SCAR^k13811, WASp¹ has larval ventral nerve cord phenotype

(Zallen et al., 2002)

SCAR^k13811, WASp¹ has larval ventral nerve cord commissure phenotype

(Zallen et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Presence of Whamy^Δ109/Whamy^Δ109 enhances early adulthood lethality seen in in WASp¹/WASp¹, whereby double mutants are late-embryonic lethal with few larval escapers.

Whamy^Δ109/+ shifts lethality from late pupal or adult to early larval stages in WASp¹/Df(3R)3450 transheterozygotes. Whamy^Δ109/+ enhances the decrease of macrochaetes on the head and notum of WASp¹/Df(3R)3450 immature adults and the increased number of neurons per sensory organ in WASp¹/Df(3R)3450 (24h APF) pupal notae.

Expression of Whamy^{Scer\UAS.T:Avic\GFP-EGFP} driven by Scer\GAL4^sca.PU partially suppresses the loss of sensory macrochaetes from the heads of WASp¹/WASp¹ flies.

WASp¹/WASp¹;Whamy^Δ109/Whamy^Δ109 double mutant stage 16 embryos have strong defects in muscle size and morphology, including a reduction in the number of nuclei contained in segmental border muscle (disturbed myoblast fusion). WASp¹/WASp¹;Whamy^Δ109/Whamy^Δ109 double mutant stage 11 embryos have similar numbers of founder cells compared to wild type.

(Brinkmann et al., 2016)

Heterozygosity for WASp¹ does not suppress the split microchaete phenotype caused by expression of Abp1^{Scer\UAS.T:Myr-Src64B,T:Avic\GFP} under the control of Scer\GAL4^sca.PU.

(Koch et al., 2012)

nwk¹ enhances the overgrown neuromuscular junction phenotype seen in WASp¹/Df(3R)3450 third instar larvae.

WASp¹/Df(3R)3450 enhances the overgrown neuromuscular junction phenotype seen in homozygous nwk¹ third instar larvae.

WASp¹/Df(3R)3450 partially suppresses the small neuromuscular junction phenotype seen in wit^A12/wit^B11 mutants.

Expression of tkv^{Q253D.Scer\UAS.cNb} under the control of Scer\GAL4^n-syb.PS does not enhance the neuromuscular junction overgrowth phenotype seen in WASp¹/Df(3R)3450 third instar larvae.

WASp¹/Df(3R)3450 does not enhance the neuromuscular junction overgrowth phenotype seen in tweek¹/tweek² third instar larvae.

(Khuong et al., 2010)

The increase in bouton number/muscle area and satellite bouton number at the neuromuscular junction seen in third instar WASp¹/Df(3R)3450 larvae is partially suppressed by RhoGAP92B¹/RhoGAP92B².

The decrease in bouton number/muscle area and satellite bouton number at the neuromuscular junction which is seen in third instar RhoGAP92B¹/RhoGAP92B² larvae is significantly suppressed by WASp¹/+.

(Nahm et al., 2010)

WASp¹/Cip4¹ double heterozygotes exhibit an increase in total bouton number, satellite bouton formation and neuromuscular junction length.

Cdc42²/+; Cip4¹/+; WASp¹/+ trans-heterozygous larvae exhibit an increase in total bouton number, neuromuscular junction length and in particular satellite bouton formation.

(Nahm et al., 2010)

WASp¹ nwk² double mutant larvae display more severe neuromuscular junction (NMJ) phenotypes than either single mutant with respect to bouton number, branch formation and NMJ length. The complexity of hyperbranching at the NMJ is also dramatically increased in the double mutants.

(Coyle et al., 2004)

In the ventral nerve cord of SCAR^K13811/+; WASp¹/Df(3R)3450 embryos, breaks occur in both longitudinal and commissural axon bundles, and axons are medially displaced. In the ventral nerve cord of SCAR^K13811/SCAR^K13811; WASp¹/+ embryos, there is an severe depletion of axons in both longitudinal and commissural bundles, often leading to gaps.

(Zallen et al., 2002)

WASp¹/Df(3R)3450 N^l1N-ts1 flies show an enhancement of the WASp¹/Df(3R)3450 bristle loss phenotype; the flies lack practically all bristles on regions of the cuticle such as the thorax. The loss of interommatidial bristles seen in WASp¹/Df(3R)3450 flies is almost completely suppressed by H³. A significant restoration of the bristle pattern is seen in WASp¹/Df(3R)3450 flies expressing N^int.hs, particularly in the abdomen. Large numb² clones in the head of WASp¹/Df(3R)3450 animals rarely show the numb² multiple socket phenotype, while the WASp¹/Df(3R)3450 smooth cuticle phenotype predominates, indicating that WASp is epistatic to numb.

(Ben-Yaacov et al., 2001)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\PLCD1^{PH.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^n-syb.PS does not enhance the neuromuscular junction overgrowth seen in WASp¹/Df(3R)3450 third instar larvae.

Expression of Hsap\PLCD1^{PH.S39R.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^n-syb.PS does not enhance the neuromuscular overgrowth seen in WASp¹/Df(3R)3450 third instar larvae.

(Khuong et al., 2010)

Complementation and Rescue Data

Fails to complement

WASp³

(Zelhof and Hardy, 2004)

Rescued by

Df(3R)3450/WASp¹ is rescued by WASp^H242D.UAS/Scer\GAL4^nSyb.PS

(Khuong et al., 2010)

Df(3R)3450/WASp¹ is rescued by WASp^UAS.cBa/Scer\GAL4^nSyb.PS

(Khuong et al., 2010)

WASp¹ is rescued by WASp^UAS.C.GFP/Scer\GAL4^kirre.PR

(Massarwa et al., 2007)

Df(3R)3450/WASp¹ is rescued by Scer\GAL4^arm.PS/WASp^H242D.UAS

(Tal et al., 2002)

Df(3R)3450/WASp¹ is rescued by WASp^{ΔB.H242D.UAS}/Scer\GAL4^arm.PS

(Tal et al., 2002)

Df(3R)3450/WASp¹ is rescued by WASp^ΔB.UAS/Scer\GAL4^arm.PS

(Tal et al., 2002)

Df(3R)3450/WASp¹ is rescued by WASp^ΔCRIB.UAS/Scer\GAL4^arm.PS

(Tal et al., 2002)

Df(3R)3450/WASp¹ is rescued by WASp^UAS.cBa/Scer\GAL4^T80

(Tal et al., 2002)

Df(3R)3450/WASp¹ is rescued by Scer\GAL4^arm.PS/WASp^UAS.cBa

(Tal et al., 2002)

Partially rescued by

WASp¹ is partially rescued by WASp^UAS.cBa/Scer\GAL4^elav.PLu

(Coyle et al., 2004)

Df(3R)3450/WASp¹ is partially rescued by WASp^ΔA.UAS/Scer\GAL4^arm.PS

(Tal et al., 2002)

Not rescued by

Df(3R)3450/WASp¹ is not rescued by WASp^ΔBR.UAS/Scer\GAL4^nSyb.PS

(Khuong et al., 2010)

Df(3R)3450/WASp¹ is not rescued by WASp^{ΔBR-H242D.UAS}/Scer\GAL4^nSyb.PS

(Khuong et al., 2010)

Df(3R)3450/WASp¹ is not rescued by Scer\GAL4^arm.PS/WASp^ΔCA.UAS

(Tal et al., 2002)

Comments

Expression of WASp^Scer\UAS.cBa under the control of Scer\GAL4^n-syb.PS rescues the neuromuscular junction overgrowth seen in WASp¹/Df(3R)3450 third instar larvae.

Expression of WASp^{ΔB.H242D.Scer\UAS} under the control of Scer\GAL4^n-syb.PS fails to rescue the neuromuscular junction overgrowth seen in WASp¹/Df(3R)3450 third instar larvae.

Expression of WASp^ΔB.Scer\UAS under the control of Scer\GAL4^n-syb.PS fails to rescue the neuromuscular junction overgrowth seen in WASp¹/Df(3R)3450 third instar larvae.

Expression of WASp^{H242D.Scer\UAS} under the control of Scer\GAL4^n-syb.PS rescues the neuromuscular junction overgrowth seen in WASp¹/Df(3R)3450 third instar larvae.

Expression of WASp¹/Df(3R)3450 does not enhance the enlarged neuromuscular junction phenotype seen when Hsap\PLCD1^{PH.Scer\UAS.T:Avic\GFP-EGFP} is expressed under the control of Scer\GAL4^n-syb.PS in homozygous nwk¹ third instar larvae.

(Khuong et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51657

w^*; e¹ WASp¹/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

WASP¹

(Galy et al., 2011)

WASp¹

(Stark et al., 2021, Kim et al., 2019, Schejter, 2006.7.3)

Wsp¹

(Rotkopf et al., 2011, Massarwa et al., 2007, Tal et al., 2002, Zallen et al., 2002, Ben-Yaacov et al., 2001)

wasp¹

(Kim et al., 2021, Brinkmann et al., 2016, Koch et al., 2012, Stephan et al., 2011, Li and Baker, 2007, Schafer et al., 2007, Bogdan et al., 2005)

wasp^Δ1

(Babatz et al., 2018)

wsp¹

(Krämer et al., 2023, Del Signore et al., 2021, Brinkmann et al., 2016, Giuliani et al., 2013, Khuong et al., 2010, Nahm et al., 2010, Nahm et al., 2010, Corrigall et al., 2007, Coyle et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (27)

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