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General Information
Symbol
Dmel\ZirBG00267
Species
D. melanogaster
Name
FlyBase ID
FBal0122312
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
CG11376BG00267
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion within the open reading frame, within the codon for amino acid 123.

Insertion components
P{GT1}ZirBG00267
Product class / Tool use(s)
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mutant larvae show reduced encapsulation (10%) of eggs when parasitised by the avirulent L. boulardi wasp strain G486. Plasmatocytes fully cover the egg, while lamellocytes partially cover it.

Homozygous larvae show a reduced rate of encapsulation (27%) of eggs of the avirulent wasp strain L. boulardi G486 compared to the rate seen in wild-type larvae. By 20 hours post-parasitisation, the wasp eggs are completely encapsulated by plasmatocytes, which are properly spread around the wasp egg chorion, as occurs in wild type. However, at 38-40 hours post-parasitisation, eggs from mutant larvae have fewer lamellocytes adhered to them than those from wild-type larvae. 40% of the hemocytes bled from these mutant larvae have abnormal elongated extensions.

Isolated plasmatocytes from homozygous larvae show a reduced ability to phagocytose E. coli and S. aureus in a phagocytosis assay.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

ZirBG00267/+ ; Rac2Δ/+ double heterozygous larvae have a reduced rate of encapsulation (42%) of eggs of the avirulent wasp strain L. boulardi G486 compared to the rate seen in either single heterozygote.

ZirBG00267/+ ; Cdc422/+ double heterozygous larvae have a reduced rate of encapsulation (37%) of eggs of the avirulent wasp strain L. boulardi G486 compared to the rate seen in either single heterozygote.

Expression of either Cdc42V12.Scer\UAS or Rac2Scer\UAS.cARa under the control of Scer\GAL4He.PZ rescues the reduced rate of encapsulation of eggs of the avirulent wasp strain L. boulardi G486 that is seen in ZirBG00267 larvae.

Expression of either Cdc42V12.Scer\UAS or Rac2Scer\UAS.cARa under the control of Scer\GAL4He.PZ rescues the reduced phagocytosis index against E. coli and S. aureus seen in isolated plasmatocytes from homozygous ZirBG00267 larvae.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
ZirBG00267
Name Synonyms
Secondary FlyBase IDs
    References (3)