FlyBase Allele Report: Dmel\lwr[14]

General Information

Symbol

Dmel\lwr¹⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0122958

Feature type

allele

Associated gene

Dmel\lwr

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

loss of function allele

(Sun et al., 2003)

Mutagen

Delta2-3 transposase

(Apionishev et al., 2001)

Progenitor genotype

P{PZ}lwr⁰⁵⁴⁸⁶

(Apionishev et al., 2001)

Caused by aberration

Df(2L)lwr¹⁴

Cytology

Description

1136bp deletion starting from the site of the P{PZ}lwr⁰⁵⁴⁸⁶ insertion and extending proximally, removing part of the first exon of lwr and extending into Spp.

(Apionishev et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of second instar larval stage (with lwr¹³)

(Sun et al., 2003)

melanotic mass phenotype | larval stage (with lwr⁵)

(Chiu et al., 2005)

melanotic mass phenotype | larval stage | partially (with lwr^4-3)

(Chiu et al., 2005)

some die during second instar larval stage (with lwr¹³)

(Sun et al., 2003)

Phenotype Manifest In

embryonic/larval crystal cell (with lwr^4-3)

(Chiu et al., 2005)

embryonic/larval crystal cell (with lwr⁵)

(Chiu et al., 2005)

hemocyte & nucleus | supernumerary (with lwr^4-3)

(Chiu et al., 2005)

hemocyte | increased number (with lwr⁵)

(Chiu et al., 2005)

lamellocyte | increased number (with lwr^4-3)

(Chiu et al., 2005)

lamellocyte | increased number (with lwr⁵)

(Chiu et al., 2005)

Detailed Description

Statement

Reference

Over 90% of lwr¹⁴/lwr⁵ larvae have melanotic tumours, which are surrounded by lamellocytes, while only ~20% of lwr^4-3/lwr¹⁴ larvae show this phenotype. Four days after egg lay, percentages of circulating hemocytes and lamellocytes are significantly higher in lwr¹⁴/lwr⁵ mutants than in controls, while lwr^4-3/lwr¹⁴ mutants show high lamellocyte percentages, but have circulating hemocyte levels that are within control range. More than 6% of lwr^4-3/lwr¹⁴ hemocytes, in both lymph glands and in circulation, are multinucleate and some hemocytes are abnormally large. There is no significant population of multinucleate hemocytes in lwr⁵/lwr¹⁴ mutants. There is a significant reduction in crystal cells in both lwr¹⁴/lwr⁵ (>20-fold) and lwr^4-3/lwr¹⁴ (5- to 10-fold) mutants.

(Chiu et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

lwr¹⁰

(Sun et al., 2003)

lwr¹³

(Sun et al., 2003)

Rescued by

lwr¹⁴ is rescued by lwr^+t2.4

(Casso et al., 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

lwr¹⁴

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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