FlyBase Allele Report: Dmel\Pvf1[EP1624]

(Bakopoulos et al., 2022, Tsai et al., 2022, Bilak et al., 2014, Poukkula et al., 2011, Franciscovich et al., 2008, Cho et al., 2002, Munier et al., 2002, Duchek et al., 2001, Gene Disruption Project members, 2001-, Kraut et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

lethal - all die before end of pupal stage

(Munier et al., 2002)

partially lethal

(Macias et al., 2004)

semi-fertile

(Macias et al., 2004)

some die during pupal stage

(Munier et al., 2002)

viable

(McDonald et al., 2003, Duchek et al., 2001)

Phenotype Manifest In

anterior fascicle & synapse, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

border follicle cell

(Poukkula et al., 2011, Mathieu et al., 2007, McDonald et al., 2006, McDonald et al., 2003, Duchek et al., 2001)

border follicle cell, with Scer\GAL4^slbo.2.6

(Poukkula et al., 2011)

ejaculatory bulb

(Macias et al., 2004)

embryonic dorsal epidermis

(Macias et al., 2004)

larval intersegmental nerve, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

male terminalia

(Macias et al., 2004)

Detailed Description

Statement

Reference

Border follicle cell clusters in homozygous females show significantly reduced net cluster movement compared to wild type in the early (but not in the late) phase of posterior migration. The cells show increased tumbling in the early phase of posterior migration. The number of cellular extensions from the front of the border cell cluster is reduced compared to wild type in the early phase of posterior migration.

(Poukkula et al., 2011)

Border cells are motile but much less directional in a Pvf1^EP1624 mutant background. Migration is delayed for about 95% of border cell clusters in Pvf1^EP1624 mutants.

(Janssens et al., 2010)

Mutants show mild defects in posterior migration of border follicle cells towards the oocyte.

(Mathieu et al., 2007)

13% of Pvf1^EP1624 egg chambers show border cell migration defects.

(McDonald et al., 2006)

Males show rotated external terminalia. The phenotype shows variable penetrance and expressivity, both of which increase at higher temperatures. Direction of rotation of spermiduct is unaffected, though the sperm pump lies on the left rather than the wild type right. There is some degree of pupal lethality and adults show reduced fertility. Individuals occasionally show a failure of dorsal closure.

(Macias et al., 2004)

Border follicle cells show migration defects in homozygous Pvf1^EP1624 stage 10 egg chambers; in 81% of egg chambers they have migrated 76-100% the normal distance, in 8% they have migrated 51-75% the normal distance, in 6% they have migrated 26-50% the normal distance and in 5% they have migrated 0-25% the normal distance. Pvf1^EP1624 has little effect on the border cell migration phenotype seen in egg chambers expressing one of copy of Pvf1^Scer\UAS.cMa under the control of Scer\GAL4^Act5C.PI in anterior follicle cell clones. If the egg chambers are also heterozygous or homozygous for Pvf1^EP1624, in 80% of cases the border cell clusters migrate normally to the oocyte.

(McDonald et al., 2003)

Mutant larvae show no obvious defects in blood cell counts. Mutant pupae show 40-60% lethality.

(Munier et al., 2002)

Homozygotes exhibit a weak border cell migration phenotype.

(Duchek et al., 2001)

Mutant larvae expressing Pvf1^EP1624 under the control of Scer\GAL4^elav-C155 have reduced or abnormal synapses. Mutant larvae expressing Pvf1^EP1624 under the control of Scer\GAL4^elav-C155 have ISN neuron pathfinding defects.

(Kraut et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Pvf1^EP1624, Scer\GAL4^ptc-559.1 is a suppressor of radiation resistant | cell non-autonomous phenotype of E2f1^RNAi.UAS.cJa, Scer\GAL4^ptc-559.1

(Bilak et al., 2014)

Other

Statement

Reference

Df(3L)Krn-27-7-B, Pvf1^EP1624, spi¹ has sterile phenotype

(McDonald et al., 2006)

Df(3L)Krn-27-7-B, Pvf1^EP1624, spi¹ has partially lethal phenotype

(McDonald et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

Pvf1^EP1624, Scer\GAL4^slbo.2.6 has border follicle cell phenotype, enhanceable by Egfr^UAS.cBa, Scer\GAL4^slbo.2.6

(Poukkula et al., 2011)

Pvf1^EP1624 has border follicle cell phenotype, enhanceable by hep^Act.UAS/Scer\GAL4^slbo.2.6

(Mathieu et al., 2007)

NOT Enhanced by

Statement

Reference

Pvf1^EP1624 has border follicle cell phenotype, non-enhanceable by Df(3L)Krn-27-7-B

(McDonald et al., 2006)

Pvf1^EP1624 has border follicle cell phenotype, non-enhanceable by spi¹

(McDonald et al., 2006)

Enhancer of

Statement

Reference

Pvf1^EP1624, Scer\GAL4^slbo.2.6 is an enhancer of border follicle cell phenotype of Egfr^UAS.cBa, Scer\GAL4^slbo.2.6

(Poukkula et al., 2011)

Pvf1^EP1624, Scer\GAL4^slbo.2.6 is an enhancer of border follicle cell phenotype of Egfr^DN.UAS, Scer\GAL4^slbo.2.6

(Duchek et al., 2001)

Suppressor of

Statement

Reference

Pvf1^EP1624, Scer\GAL4^ptc-559.1 is a suppressor of wing disc | cell non-autonomous phenotype of E2f1^RNAi.UAS.cJa, Scer\GAL4^ptc-559.1

(Bilak et al., 2014)

Pvf1^EP1624, Scer\GAL4^ey.3.5.Exel is a suppressor of eye phenotype of Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS

(Franciscovich et al., 2008)

Other

Statement

Reference

Pvf1^EP1624, grk^HF, spi¹ has egg chamber phenotype

(McDonald et al., 2006)

Pvf1^EP1624, tai[+]/tai^61G1 has border follicle cell phenotype

(McDonald et al., 2003)

Pvf1^EP1624, tai^61G1 has border follicle cell phenotype

(McDonald et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of Pvf1^EP1624 attenuates the protective effect on surrounding cells seen when cell death is initiated through expression of E2f1^{dsRNA.Scer\UAS.cJa} under the control of Scer\GAL4^ptc-559.1.

(Bilak et al., 2014)

The effect on border cell cluster migration of expressing Pvr^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^c522 in a single border follicle cell in a Egfr^dsRNA.slbo Pvf1^EP1624 background (to remove endogenous guidance information) has been studied. These clusters are dynamic and display disordered movement including cluster tumbling. In those border cell clusters where the single Pvr^{Scer\UAS.T:Avic\GFP}-expressing cell is located at the front of the cluster, the cluster on average moves forward, when the Pvr^{Scer\UAS.T:Avic\GFP}-expressing cell is in the back, the cluster on average moves relatively more backwards and when the Pvr^{Scer\UAS.T:Avic\GFP}-expressing cell is on the side of the cluster, border cell migration is unaltered compared to control clusters without a Pvr^{Scer\UAS.T:Avic\GFP}-expressing cell.

(Inaki et al., 2012)

Expression of Pvf1^EP1624 suppresses the small eye phenotype associated with kay^Fbz.Scer\UAS, when both are expressed under the control of Scer\GAL4^ey.3.5.Exel.

(Franciscovich et al., 2008)

Expression of hep^Act.Scer\UAS under the control of Scer\GAL4^slbo.2.6 enhances the severity of the posterior migration defects seen in Pvf1^EP1624 mutant border follicle cells.

(Mathieu et al., 2007)

Pvf1^EP1624; Df(3L)Krn-27-7-B double mutant egg chambers show no enhancement of the border cell migration defects seen in Pvf1^EP1624 single mutants. Likewise, no enhancement is seen in Pvf1^EP1624; spi¹ double mutants.

Pvf1^EP1624; spi¹; Df(3L)Krn-27-7-B triple mutants are poorly viable or sterile.

The egg chambers of Pvf1^EP1624; spi¹; grk^HF females do not develop normally.

(McDonald et al., 2006)

About 6% of Pvf1^EP1624 tai^61G1 double heterozygous egg chambers have border cell migration defects.

(McDonald et al., 2003)

The combination of Pvf1^EP1624, and Egfr^DN.Scer\UAS (driven by Scer\GAL4^slbo.2.6) leads to strong effect. Border cells are not able to reach the oocyte by stage 10, and they also show a low level of "off track" migration.

(Duchek et al., 2001)

Xenogenetic Interactions

Statement

Reference

The effect on border cell cluster migration of expressing Pvr::Hsap\EGFR^{hE-P.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^c522 in a single border follicle cell in a Egfr^dsRNA.slbo Pvf1^EP1624 background (to remove endogenous guidance information) has been studied. Overall disordered, or tumbling border cell movements are seen in these clusters. In those border cell clusters where the single Pvr::Hsap\EGFR^{hE-P.Scer\UAS.T:Avic\GFP}-expressing cell is located at the front of the cluster, the cluster on average moves forward, when the Pvr::Hsap\EGFR^{hE-P.Scer\UAS.T:Avic\GFP}-expressing cell is in the back, the cluster on average moves relatively more backwards.

(Inaki et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11450

w¹¹¹⁸ P{EP}Pvf1^EP1624

Notes on Origin

Discoverer

Comments

Excision of the P{EP} element reverts the mutant phenotype.

(Duchek et al., 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

EP1624^EP1624

Pvf1¹⁶²⁴

(Bakopoulos et al., 2022, Inaki et al., 2012, Poukkula et al., 2011, Janssens et al., 2010, Bianco et al., 2007, Mathieu et al., 2007, McDonald et al., 2006, Munier et al., 2002, Duchek et al., 2001)

Pvf1^EP1624

(Tsai et al., 2022, Pan and O'Connor, 2021, Bilak et al., 2014)

Pvf1^null

(Tsai et al., 2022)

Vegf17E^EP1624

(Cho et al., 2002)

pvf1^EP1624

(Learte et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (19)

Report Sections

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