FlyBase Allele Report: Dmel\tsu[EP567]

General Information

Symbol

Dmel\tsu^EP567

Species

D. melanogaster

Name

FlyBase ID

FBal0128984

Feature type

allele

Associated gene

Dmel\tsu

Associated Insertion(s)

P{EP}tsu^EP567

Carried in Construct

Also Known As

EP(2)0567, tsu¹

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Mohr and Boswell, 2002, Gene Disruption Project members, 2001-, Hachet and Ephrussi, 2001, Mohr et al., 2001)

Progenitor genotype

Associated Insertion(s)

P{EP}tsu^EP567

Cytology

Description

P{EP} insertion 70bp upstream of the predicted start codon.

(Hachet and Ephrussi, 2001)

P{EP} insertion in the 5' UTR, 72bp upstream of the first translation start site.

(Mohr et al., 2001)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:9,110,823..9,110,823 [+]

(Wu et al., 2019, Couthouis et al., 2011, Vicente-Crespo et al., 2008, Sofola et al., 2007, Mohr and Boswell, 2002, Gene Disruption Project members, 2001-, Hachet and Ephrussi, 2001, Mohr et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with Df(2R)BSC271)

(Kahsai and Cook, 2018, Kahsai et al., 2016)

lethal | recessive

(Hachet and Ephrussi, 2001)

lethal - all die during embryonic stage | germline clone | recessive

(Mohr et al., 2001)

some die during embryonic stage | recessive

(Mohr et al., 2001)

Phenotype Manifest In

egg | germline clone

(Hachet and Ephrussi, 2001, Mohr et al., 2001)

Detailed Description

Statement

Reference

Overexpression of tsu^EP567 in the eye under the control of Scer\GAL4^GMR.PF does not cause any apparent neurodegeneration in the retina.

(Couthouis et al., 2011)

Females carrying homozygous germline clones lay eggs which are smaller than wild type and are unfertilised. The actin cytoskeleton (including the ring canals) appears normal in mutant egg chambers and mutant oocytes show the normal anterolateral accumulation of microtubules.

(Hachet and Ephrussi, 2001)

Eggs and embryos derived from females with homozygous follicle cell clones are indistinguishable from wild type. Females containing homozygous germ-line clones lay defective eggs and embryos derived from these eggs fail to hatch.

(Mohr et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

tsu^EP567 is a non-enhancer of visible phenotype of Scer\GAL4^sev.PU, Zzzz\CTG^i480.UAS.cGa

(Garcia-Lopez et al., 2008)

Suppressor of

Statement

Reference

tsu[+]/tsu^EP567 is a suppressor of visible phenotype of Ras85D^V12.sev

(Ashton-Beaucage et al., 2010)

NOT Suppressor of

Statement

Reference

tsu^EP567 is a non-suppressor of visible phenotype of Scer\GAL4^sev.PU, Zzzz\CTG^i480.UAS.cGa

(Garcia-Lopez et al., 2008)

Phenotype Manifest In

Enhancer of

Statement

Reference

tsu^EP567 is an enhancer of eye phenotype of Scer\GAL4^hs.2sev, mbl^C.UAS

(Vicente-Crespo et al., 2008)

NOT Enhancer of

Statement

Reference

tsu^EP567 is a non-enhancer of eye phenotype of Scer\GAL4^sev.PU, Zzzz\CTG^i480.UAS.cGa

(Garcia-Lopez et al., 2008)

Suppressor of

Statement

Reference

tsu[+]/tsu^EP567 is a suppressor of eye phenotype of Ras85D^V12.sev

(Ashton-Beaucage et al., 2010)

NOT Suppressor of

Statement

Reference

tsu^EP567 is a non-suppressor of eye phenotype of Scer\GAL4^sev.PU, Zzzz\CTG^i480.UAS.cGa

(Garcia-Lopez et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

A tsu^EP567 mutant background enhances the moderately rough eye phenotype observed upon expression of mbl^C.Scer\UAS under the control of Scer\GAL4^hs.2sev.

(Vicente-Crespo et al., 2008)

Xenogenetic Interactions

Statement

Reference

tsu^EP567 has no effect on the rough eye phenotype caused by expression of Zzzz\CTG^{i480.Scer\UAS.cGa} under the control of Scer\GAL4^sev.PU.

(Garcia-Lopez et al., 2008)

Complementation and Rescue Data

Fails to complement

tsu²

(Mohr et al., 2001)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6597

w¹¹¹⁸; P{EP}tsu^EP567 bw¹ speck¹/SM5

Notes on Origin

Discoverer

Comments

Excision of the P{EP} element can revert the lethal phenotype.

(Hachet and Ephrussi, 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

EP(2)0567

(Mohr and Boswell, 2002, Hachet and Ephrussi, 2001, Mohr et al., 2001)

tsu¹

(Ashton-Beaucage et al., 2010, Mohr et al., 2001)

tsu^EP0567

tsu^EP567

(Kahsai and Cook, 2018, Garcia-Lopez et al., 2008, Christensen and Cook, 2007.3.22, Sofola et al., 2007)

y14^EP(2)0567

(Hachet and Ephrussi, 2001)

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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