FlyBase Allele Report: Dmel\Atg1[EP3348]

General Information

Symbol

Dmel\Atg1^EP3348

Species

D. melanogaster

Name

FlyBase ID

FBal0131706

Feature type

allele

Associated gene

Dmel\Atg1

Associated Insertion(s)

P{EP}Atg1^EP3348

Carried in Construct

Also Known As

EP(3)3348, EP3348

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{EP}Atg1^EP3348

Cytology

Description

Insertion in the first exon, 5bp downstream of the first ATG codon.

(Ahantarig et al., 2009)

Insertion within the first exon, 539bp upstream of the ATG start codon.

(Toda et al., 2008)

P{EP} is inserted into the 5' UTR of Atg1, 541bp upstream of the start of the Atg1 coding region.

(Scott et al., 2004)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:12,804,983..12,804,983 [+]

(Cao et al., 2008, Toda et al., 2008, Zhu et al., 2005, Scott et al., 2004, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | dominant | embryonic stage

(Ahantarig et al., 2009)

lethal - all die before end of pupal stage | recessive

(Scott et al., 2004)

partially lethal - majority die

(Lee et al., 2007)

partially lethal - majority die | adult stage

(Toda et al., 2008)

some die during pupal stage | recessive

(Scott et al., 2004)

Phenotype Manifest In

autophagosome | conditional

(Lee et al., 2007)

larval longitudinal connective

(Ahantarig et al., 2009)

symmetrical commissure

(Ahantarig et al., 2009)

Detailed Description

Statement

Reference

Heterozygous embryos have distorted and broken longitudinal connectives.

Homozygous embryos show increased distortion of the commissures and longitudinal connectives.

(Ahantarig et al., 2009)

Expression using Scer\GAL4^ey.PU does not induce a rough eye phenotype.

(Cao et al., 2008)

About 30% of Atg1^EP3348 homozygous mutants develop into adults. No mitotic defects are evident in the larval brain and imaginal discs of mutant animals.

Atg1^EP3348 mutant animals show marked defects in the induction of autophagy and autophagosome formation under conditions of starvation.

(Lee et al., 2007)

Mutants show disruption of autophagy.

(Scott et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Atg1^EP3348 has partially lethal - majority die phenotype, suppressible by S6k[+]/S6k⁰⁷⁰⁸⁴

(Lee et al., 2007)

Atg1^EP3348 has partially lethal - majority die phenotype, suppressible by S6k[+]/S6k^l-1

(Lee et al., 2007)

Enhancer of

Statement

Reference

Atg1^EP3348, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

NOT Enhancer of

Statement

Reference

Atg1^EP3348, Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Rac1^N17.UAS, Scer\GAL4^GMR.PF

(Zhu et al., 2005)

Atg1^EP3348, Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Cdc42^L89.UAS, Scer\GAL4^GMR.PF

(Zhu et al., 2005)

Atg1^EP3348, Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PF, btl::Egfr^λ.UAS

(Zhu et al., 2005)

Atg1^EP3348, Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Pvr^λ.UAS, Scer\GAL4^GMR.PF

(Zhu et al., 2005)

Suppressor of

Statement

Reference

Atg1[+]/Atg1^EP3348 is a suppressor of abnormal body size | second instar larval stage phenotype of mTor^k17004

(Lee et al., 2007)

Atg1^EP3348/Atg1^EP3348 is a suppressor of abnormal body size | second instar larval stage phenotype of mTor^k17004

(Lee et al., 2007)

Atg1^EP3348/Atg1^EP3348 is a suppressor | partially of lethal phenotype of mTor^k17004

(Lee et al., 2007)

Atg1[+]/Atg1^EP3348 is a suppressor | partially of decreased cell size phenotype of mTor^k17004

(Lee et al., 2007)

NOT Suppressor of

Statement

Reference

Atg1^EP3348, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Rac1^N17.UAS, Scer\GAL4^GMR.PF

(Zhu et al., 2005)

Atg1^EP3348, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Cdc42^L89.UAS, Scer\GAL4^GMR.PF

(Zhu et al., 2005)

Atg1^EP3348, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, btl::Egfr^λ.UAS

(Zhu et al., 2005)

Atg1^EP3348, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Pvr^λ.UAS, Scer\GAL4^GMR.PF

(Zhu et al., 2005)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Atg1^EP3348 has autophagosome | conditional phenotype, non-suppressible by S6k[+]/S6k⁰⁷⁰⁸⁴

(Lee et al., 2007)

Atg1^EP3348 has autophagosome | conditional phenotype, non-suppressible by S6k[+]/S6k^l-1

(Lee et al., 2007)

Enhancer of

Statement

Reference

Atg1^EP3348, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

NOT Enhancer of

Statement

Reference

Atg1^EP3348, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, btl::Egfr^λ.UAS

(Zhu et al., 2005)

Atg1^EP3348, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Pvr^λ.UAS, Scer\GAL4^GMR.PF

(Zhu et al., 2005)

Suppressor of

Statement

Reference

Atg1[+]/Atg1^EP3348 is a suppressor | partially of nucleus phenotype of mTor^k17004

(Lee et al., 2007)

Atg1[+]/Atg1^EP3348 is a suppressor of fat body | second instar larval stage phenotype of mTor^k17004

(Lee et al., 2007)

Atg1[+]/Atg1^EP3348 is a suppressor of vesicle phenotype of mTor^k17004

(Lee et al., 2007)

NOT Suppressor of

Statement

Reference

Atg1^EP3348, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, btl::Egfr^λ.UAS

(Zhu et al., 2005)

Atg1^EP3348, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Pvr^λ.UAS, Scer\GAL4^GMR.PF

(Zhu et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Defects in autophagosome formation in starved Atg1^EP3348 homozygous larvae are not rescued in a S6k⁰⁷⁰⁸⁴ or S6k^l-1 heterozygous background. However, eclosion rates are increased in these animals in a dose-dependent manner. Roughly 50% of Atg1^EP3348, S6k⁰⁷⁰⁸⁴/+ eclose, increased to 70% in a Atg1^EP3348, S6k^l-1/+ genetic background.

Tor^k17004 homozygous animals in a heterozygous Atg1^EP3348 genetic background grow faster, and extend their developmental stage to mid/late third instar larval stage. Double homozygous animals survive to mid-late third instar larval stage.

Lipid vesicle aggregation in the fat body of Tor^k17004 homozygous second/early third instar larvae is suppressed in a heterozygous Atg1^EP3348 genetic background.

Tor^k17004 mutant larvae heterozygous for Atg1^EP3348 show a partially rescued cell and nuclear salivary gland size.

(Lee et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Precise excisions of the P{EP} result in viability.

(Scott et al., 2004)

Precise excision of the insertion rescues the semi-lethality.

(Toda et al., 2008)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Atg1^EP3348

(Wairkar et al., 2009)

CG10967^EP3348

DmATG1¹

(Lee et al., 2007)

EP3348

(Lee et al., 2007)

atg¹

(Castillo-Quan et al., 2016)

l(3)00305^EP3348

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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