FlyBase Allele Report: Dmel\RPA2[KG00759]

General Information

Symbol

Dmel\RPA2^KG00759

Species

D. melanogaster

Name

FlyBase ID

FBal0136112

Feature type

allele

Associated gene

Dmel\RPA2

Associated Insertion(s)

P{SUPor-P}RPA2^KG00759

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Crest et al., 2007)

Mutagen

P-element activity

(Levis, 2002.6.25)

Progenitor genotype

Associated Insertion(s)

P{SUPor-P}RPA2^KG00759

Cytology

Description

P{SUPor-P} insertion in the third exon.

(Crest et al., 2007)

P{SUPor-P} element in an exon of CG9273 which is nested in an intron of "CG18078". <up>FlyBase curator comment: CG18078 split into CG33317, CR33318 and CR33319 in release 3.2 of the genome annotation. The P{SUPor-P}CG9273^KG00759 insertion is not within the transcription unit of CG33317, CR33318 or CR33319</up>.

(Levis, 2002.6.25)

Allele components

Component

Use(s)

Inserted element

P{SUPor-P}

insertional mutagenesis tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:20,925,806..20,925,806 [+]

(Li et al., 2021, Crest et al., 2007, Levis, 2002.6.25)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of second instar larval stage | recessive

(Crest et al., 2007)

some die during second instar larval stage | recessive

(Crest et al., 2007)

Phenotype Manifest In

larval outer optic anlage | somatic clone

(Zhou and Luo, 2013)

Detailed Description

Statement

Reference

Homozygous clones in the outer proliferation center (OPC) neuroepithelium are small and composed of only a few cells. The cells retain columnar neuroepithelium cell morphology. Some of the mutant cells are extruded from the neuroepithelium and basally enter the medulla cortex. These extruded cells change morphology.

(Zhou and Luo, 2013)

Homozygotes hatch at the same rate as wild-type embryos, but arrest at the second instar with a normal larval cuticle pattern.

(Crest et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

RPA2^KG00759/CG9273[+] is a suppressor of mitotic domain 1 | embryonic cycle 14 phenotype of CycB^+t10

(Crest et al., 2007)

NOT Suppressor of

Statement

Reference

RPA2^KG00759/CG9273[+] is a non-suppressor of mitotic domain 1 | embryonic cycle 10 phenotype of CycB^+t10

(Crest et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

97% of embryos derived from females carrying four copies of CycB^+t10 (as well as two wild-type copies of CycB⁺) and also heterozygous for RPA2^KG00759 have a normal phenotype at cycle 14, while only 24% of these embryos have a normal phenotype at cycle 10.

(Crest et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

RPA2³

(Crest et al., 2007)

RPA2²

(Crest et al., 2007)

Comments

Images (0)