FlyBase Allele Report: Dmel\shot[LC.UAS.GFP]

General Information

Symbol

Dmel\shot^LC.UAS.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0137087

Feature type

allele

Associated gene

Dmel\shot

Associated Insertion(s)

Carried in Construct

P{UAS-shot.L(C)-GFP}

Also Known As

ΔCalp1, UAS-shot L(C)-GFP

Key Links

Transgenic product class

wild_type

(Lee and Kolodziej, 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Lee and Kolodziej, 2002)

Mutagen

in vitro construct

(Lee and Kolodziej, 2002, Lee and Kolodziej, 2002)

Progenitor genotype

Carried in construct

P{UAS-shot.L(C)-GFP}

Cytology

Description

UAS regulatory sequences drive expression of a form of shot that lacks the first Calponin domain.

(Bottenberg et al., 2009)

Lacks the F-actin-binding first Calponin domain of the shot protein.

(Sanchez-Soriano et al., 2009)

UAS regulatory sequences drive expression of the shot L(C) isoform tagged with GFP.

(Lee and Kolodziej, 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

shot

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

terminal tracheal cell | embryonic stage, with Scer\GAL4^btl.PU

(Ricolo and Araujo, 2020)

tracheal lumen | embryonic stage | increased number, with Scer\GAL4^btl.PU

(Ricolo and Araujo, 2020)

Detailed Description

Statement

Reference

Expression of shot^{LC.Scer\UAS.T:Avic\GFP} in embryonic motorneurons or tendon cells in a wild-type background does not engender any obvious dominant negative effects.

(Bottenberg et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^RapGAP1-OK6/shot^LC.UAS.GFP is a suppressor of abnormal neuroanatomy | larval stage phenotype of Patronin^EY05252/Df(2R)BSC355

(Gao et al., 2024)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^btl.PU/shot^LC.UAS.GFP is an enhancer of terminal tracheal cell | embryonic stage phenotype of Rca1^G012

(Ricolo and Araujo, 2020)

Scer\GAL4^btl.PU/shot^LC.UAS.GFP is an enhancer of tracheal lumen | embryonic stage | increased number phenotype of Rca1^G012

(Ricolo and Araujo, 2020)

Suppressor of

Statement

Reference

Scer\GAL4^RapGAP1-OK6/shot^LC.UAS.GFP is a suppressor of NMJ bouton | larval stage phenotype of Patronin^EY05252/Df(2R)BSC355

(Gao et al., 2024)

Scer\GAL4^RapGAP1-OK6/shot^LC.UAS.GFP is a suppressor of microtubule | larval stage phenotype of Patronin^EY05252/Df(2R)BSC355

(Gao et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

shot^LC.UAS.GFP/Scer\GAL4^sca-537.4 rescues shot³/Df(2R)MK1

(Sanchez-Soriano et al., 2009)

shot^LC.UAS.GFP/Scer\GAL4^btl.PS rescues shot³

(Lee and Kolodziej, 2002)

Partially rescues

Scer\GAL4^en.PU/shot^LC.UAS.GFP partially rescues shot^SF20

(Takács et al., 2017)

Scer\GAL4^sr-md710/shot^LC.UAS.GFP partially rescues shot^unspecified

(Bottenberg et al., 2009)

Fails to rescue

Scer\GAL4^btl.PU/shot^LC.UAS.GFP fails to rescue shot³

(Ricolo and Araujo, 2020)

shot^LC.UAS.GFP/Scer\GAL4^pnr.PU fails to rescue shot^SF20

(Takács et al., 2017)

Scer\GAL4^elav.PU/shot^LC.UAS.GFP fails to rescue shot^VV/shot³

(Valakh et al., 2013)

Scer\GAL4^how-24B/shot^LC.UAS.GFP fails to rescue shot^VV/shot³

(Valakh et al., 2013)

Scer\GAL4^unspecified/shot^LC.UAS.GFP fails to rescue shot^unspecified

(Bottenberg et al., 2009)

shot^LC.UAS.GFP/Scer\GAL4^sca-537.4 fails to rescue shot³/Df(2R)MK1

(Sanchez-Soriano et al., 2009)

Scer\GAL4^insc-Mz1407/shot^LC.UAS.GFP fails to rescue shot³

(Lee et al., 2007)

Scer\GAL4^insc-Mz1407/shot^LC.UAS.GFP fails to rescue shot³

(Lee and Kolodziej, 2002)

Comments

The expression of shot^{LC.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^pnr.PU fails to rescue the increased length:width ratio of the dorsal hole during dorsal closure of shot^SF20 homozygous embryos; expression under the control of Scer\GAL4^en.PU rescues the long and bent microtubules at the apical of the dorsal-most epithelial cells during dorsal closure of shot^SF20 homozygous embryos, but the cell body microtubules appear bundled, as compared to mutant and control embryos.

(Takács et al., 2017)

Expression of shot^{LC.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^elav.PU fails to rescue the increase in bouton number seen at the neuromuscular junction in shot³/shot^VV third instar larvae.

(Valakh et al., 2013)

Expression of shot^{LC.Scer\UAS.T:Avic\GFP} in motorneurons fails to rescue the dendritic morphology in shot^unspecified mutant embryos.

Scer\GAL4^sr-md710 driven expression of shot^{LC.Scer\UAS.T:Avic\GFP} almost completely rescues the shot^unspecified tendon cell phenotype.

(Bottenberg et al., 2009)

Scer\GAL4^sca-537.4-mediated expression of shot^{LC.Scer\UAS.T:Avic\GFP} fails to restore the microtubule unbundling and axonal length phenotypes of shot³/Df(2R)MK1 primary neuronal cultures.

Scer\GAL4^sca-537.4-mediated expression of shot^{LC.Scer\UAS.T:Avic\GFP} fully rescues the reduced filopodia phenotype of shot³/Df(2R)MK1 primary neuronal cultures.

(Sanchez-Soriano et al., 2009)

(with shot<up>LA-ΔGAS2.Scer\UAS. T:Avic \GFP</up>) Fails to rescue: shot³ { Scer\GAL4¹⁴⁰⁷ } Expression of shot^{LC.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4¹⁴⁰⁷ fails to rescue the motor and sensory axon extension defects seen in shot³ mutants. Coexpression of shot^{LA-ΔGAS2.Scer\UAS.T:Avic\GFP} and shot^{LC.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4¹⁴⁰⁷ fails to rescue the motor and sensory axon extension defects seen in shot³ mutants.

(Lee and Kolodziej, 2002)

Expression of shot^{LC.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^btl.PS rescues lumen formation in the tracheal dorsal trunk and at the dorsal midline in shot³ embryos.

(Lee and Kolodziej, 2002)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

29042

w^*; P{UAS-shot.L(C)-GFP}2

29043

w^*; P{UAS-shot.L(C)-GFP}3

Notes on Origin

Discoverer