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General Information
Symbol
Dmel\shotunspecified
Species
D. melanogaster
Name
FlyBase ID
FBal0062790
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Motorneurons are shorter in mutant embryos compared to wild type. Tendon cells are disrupted in mutant embryos.

Dendrites of aCC and RP2 motorneurons in shotunspecified mutant embryos display severely reduced complexity in morphology at late embryonic stages compared to controls. In contrast, at the onset of dendrite formation (embryonic stage 15 = 12 hours), there are no obvious aberrations in shotunspecified mutant animals compared to wild-type.

In late stage embryos, shotunspecified mutant tendon cells are dramatically elongated, held together only through their persisting apico-basal F-actin arrays.

In shotunspecified mutants, initial formation of the ectodermal keyhole region is normal, but the inward movement of these cells into the endodermal keyhole domain fails to occur. Furthermore, the rim of the proventriculus is significantly reduced in size.

Mutants show no gross defects in anterior-posterior or dorsal-ventral patterning.

Homozygotes die as early larvae. Homozygous clones in the wing produce discrete, round blisters of variable size. These blisters can be located anywhere on the wing. Wing venation is normal. Homozygous embryos show no gross morphological abnormalities at hatching. Hatched larvae fail to grow.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference

shotunspecified/shot[+] is an enhancer of visible | recessive phenotype of mys8

Other
Statement
Reference
Phenotype Manifest In
Enhancer of
Statement
Reference

shotunspecified/shot[+] is an enhancer of wing phenotype of mys8

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

shotunspecified/+ ; Eb104524/+ double heterozygous embryonic neurons in primary culture show shortening of axons and increase disorganisation of the microtubules compared to either single heterozygote.

Shows a weak genetic interaction with mys8; the frequency of wing blisters is increased from approximately 10% in mys8 single hemizygotes to approximately 20% in mys8 hemizygotes that are also heterozygous for shotunspecified. Shows no interaction with mysb9.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of shotLA.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4eve.RN2O significantly rescues the motor neuron stall phenotype seen in shotunspecified embryos in vivo.

Expression of either shotRE-ΔCtail.Scer\UAS.T:Avic\GFP or shotRE-3MtLSmut.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4eve.RN2O does not rescue the motor neuron stall phenotype seen in shotunspecified embryos in vivo.

Expression of either shotLA.Scer\UAS.T:Avic\GFP or shotRE-3MtLSmut.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4sr-md710 restores tendon cell integrity in shotunspecified embryos.

Expression of shotRE-ΔCtail.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4sr-md710 fails to rescue tendon cell integrity in shotunspecified embryos.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
shotunspecified
unspecified
Name Synonyms
Secondary FlyBase IDs
    References (5)