Motorneurons are shorter in mutant embryos compared to wild type. Tendon cells are disrupted in mutant embryos.
Dendrites of aCC and RP2 motorneurons in shotunspecified mutant embryos display severely reduced complexity in morphology at late embryonic stages compared to controls. In contrast, at the onset of dendrite formation (embryonic stage 15 = 12 hours), there are no obvious aberrations in shotunspecified mutant animals compared to wild-type.
In late stage embryos, shotunspecified mutant tendon cells are dramatically elongated, held together only through their persisting apico-basal F-actin arrays.
In shotunspecified mutants, initial formation of the ectodermal keyhole region is normal, but the inward movement of these cells into the endodermal keyhole domain fails to occur. Furthermore, the rim of the proventriculus is significantly reduced in size.
Mutants show no gross defects in anterior-posterior or dorsal-ventral patterning.
Homozygotes die as early larvae. Homozygous clones in the wing produce discrete, round blisters of variable size. These blisters can be located anywhere on the wing. Wing venation is normal. Homozygous embryos show no gross morphological abnormalities at hatching. Hatched larvae fail to grow.