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General Information
Symbol
Dmel\shotLA-ΔEF-hand.UAS.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0137093
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ΔEF
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Deletion of sequences encoding shot amino acids 4711 to 4849 (coordinates according to accession number AAF24343). This removes the two EF-hand motifs.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of shotLA-ΔEF-hand.Scer\UAS.T:Avic\GFP in embryonic motorneurons or tendon cells in a wild-type background does not engender any obvious dominant negative effects.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The expression of shotLA-ΔEF-hand.Scer\UAS.T:Avic\GFP rescues both the increased length:width ratio of the dorsal hole (expression under the control of Scer\GAL4pnr.PU) and the abnormal microtubules of the dorsal-most epithelial cells (expression under the control of Scer\GAL4en.PU), observed during dorsal closure of shotSF20 homozygous embryos.

Expression of shotLA-ΔEF-hand.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4elav.PU fails to rescue the increase in bouton number seen at the neuromuscular junction in shot3/shotVV third instar larvae.

Expression of shotLA-ΔEF-hand.Scer\UAS.T:Avic\GFP in motorneurons fails to rescue the dendritic growth phenotype of shotunspecified mutant embryos.

Scer\GAL4sr-md710 driven expression of shotLA-ΔEF-hand.Scer\UAS.T:Avic\GFP rescues the shotunspecified tendon cell phenotype.

Scer\GAL4sca-537.4-mediated expression of shotLA-ΔEF-hand.Scer\UAS.T:Avic\GFP fully restores the microtubule unbundling and axonal length phenotypes of shot3/Df(2R)MK1 primary neuronal cultures.

Scer\GAL4sca-537.4-mediated expression of shotLA-ΔEF-hand.Scer\UAS.T:Avic\GFP fails to fully rescue the reduced filopodia phenotype of shot3/Df(2R)MK1 primary neuronal cultures.

Expression of shotLA-ΔEF-hand.Scer\UAS.T:Avic\GFP under the control of Scer\GAL41407 fails to rescue the motor axon extension defects seen in shot3 mutants. Sensory axon extension is modestly improved over that seen in shot3 mutants.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
shotLA-ΔEF-hand.Scer\UAS.T:Avic\GFP
shotLA-ΔEF-hand.UAS.GFP
Name Synonyms
Secondary FlyBase IDs
    References (6)