FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\shotLA-ΔEF-hand.UAS.GFP
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General Information
Symbol
Dmel\shotLA-ΔEF-hand.UAS.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0137093
Feature type
allele
Associated gene
Dmel\shot
Associated Insertion(s)
Carried in Construct
P{UAS-shot.L(A)-ΔEF-hand-GFP}
Also Known As
ΔEF
Key Links
Transgenic product class
inframe_deletion
(Lee and Kolodziej, 2002)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Lee and Kolodziej, 2002)
Mutagen
in vitro construct
(Lee and Kolodziej, 2002)
Progenitor genotype
shotLA.UAS.GFP
(Lee and Kolodziej, 2002)
Carried in construct
P{UAS-shot.L(A)-ΔEF-hand-GFP}
Cytology
Description

Deletion of sequences encoding shot amino acids 4711 to 4849 (coordinates according to accession number AAF24343). This removes the two EF-hand motifs.

(Lee and Kolodziej, 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
shot
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of shotLA-ΔEF-hand.Scer\UAS.T:Avic\GFP in embryonic motorneurons or tendon cells in a wild-type background does not engender any obvious dominant negative effects.

      (Bottenberg et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      shotLA-ΔEF-hand.UAS.GFP/Scer\GAL4pnr.PU rescues shotSF20

      (Takács et al., 2017)

      Scer\GAL4en.PU/shotLA-ΔEF-hand.UAS.GFP rescues shotSF20

      (Takács et al., 2017)

      Scer\GAL4sr-md710/shotLA-ΔEF-hand.UAS.GFP rescues shotunspecified

      (Bottenberg et al., 2009)

      shotLA-ΔEF-hand.UAS.GFP/Scer\GAL4sca-537.4 rescues shot3/Df(2R)MK1

      (Sanchez-Soriano et al., 2009)
      Partially rescues

      shotLA-ΔEF-hand.UAS.GFP/Scer\GAL4sca-537.4 partially rescues shot3/Df(2R)MK1

      (Sanchez-Soriano et al., 2009)

      Scer\GAL4insc-Mz1407/shotLA-ΔEF-hand.UAS.GFP partially rescues shot3

      (Lee et al., 2007)

      Scer\GAL4insc-Mz1407/shotLA-ΔEF-hand.UAS.GFP partially rescues shot3

      (Lee and Kolodziej, 2002)
      Fails to rescue

      Scer\GAL4elav.PU/shotLA-ΔEF-hand.UAS.GFP fails to rescue shotVV/shot3

      (Valakh et al., 2013)

      Scer\GAL4how-24B/shotLA-ΔEF-hand.UAS.GFP fails to rescue shotVV/shot3

      (Valakh et al., 2013)

      Scer\GAL4unspecified/shotLA-ΔEF-hand.UAS.GFP fails to rescue shotunspecified

      (Bottenberg et al., 2009)
      Comments

      The expression of shotLA-ΔEF-hand.Scer\UAS.T:Avic\GFP rescues both the increased length:width ratio of the dorsal hole (expression under the control of Scer\GAL4pnr.PU) and the abnormal microtubules of the dorsal-most epithelial cells (expression under the control of Scer\GAL4en.PU), observed during dorsal closure of shotSF20 homozygous embryos.

      (Takács et al., 2017)

      Expression of shotLA-ΔEF-hand.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4elav.PU fails to rescue the increase in bouton number seen at the neuromuscular junction in shot3/shotVV third instar larvae.

      (Valakh et al., 2013)

      Expression of shotLA-ΔEF-hand.Scer\UAS.T:Avic\GFP in motorneurons fails to rescue the dendritic growth phenotype of shotunspecified mutant embryos.

      Scer\GAL4sr-md710 driven expression of shotLA-ΔEF-hand.Scer\UAS.T:Avic\GFP rescues the shotunspecified tendon cell phenotype.

      (Bottenberg et al., 2009)

      Scer\GAL4sca-537.4-mediated expression of shotLA-ΔEF-hand.Scer\UAS.T:Avic\GFP fully restores the microtubule unbundling and axonal length phenotypes of shot3/Df(2R)MK1 primary neuronal cultures.

      Scer\GAL4sca-537.4-mediated expression of shotLA-ΔEF-hand.Scer\UAS.T:Avic\GFP fails to fully rescue the reduced filopodia phenotype of shot3/Df(2R)MK1 primary neuronal cultures.

      (Sanchez-Soriano et al., 2009)

      Expression of shotLA-ΔEF-hand.Scer\UAS.T:Avic\GFP under the control of Scer\GAL41407 fails to rescue the motor axon extension defects seen in shot3 mutants. Sensory axon extension is modestly improved over that seen in shot3 mutants.

      (Lee and Kolodziej, 2002)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      shotLA-ΔEF-hand.Scer\UAS.T:Avic\GFP
      shotLA-ΔEF-hand.UAS.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (6)