Deletion of sequences encoding shot amino acids 1205 to 4599 (coordinates according to accession number AAF24343). This removes approximately 75% of the central domain, including all the spectrin repeats.
Expression of shotLA-Δrod1.Scer\UAS.T:Avic\GFP in embryonic motorneurons or tendon cells in a wild-type background does not engender any obvious dominant negative effects.
Scer\GAL4elav.PU/shotLA-Δrod1.UAS.GFP rescues shotVV/shot3
shotLA-Δrod1.UAS.GFP/Scer\GAL41407 rescues shot3
Scer\GAL4unspecified/shotLA-Δrod1.UAS.GFP partially rescues shotunspecified
Scer\GAL4sr-md710/shotLA-Δrod1.UAS.GFP partially rescues shotunspecified
Scer\GAL4how-24B/shotLA-Δrod1.UAS.GFP fails to rescue shotVV/shot3
Expression of shotLA-Δrod1.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4elav.PU rescues the increase in bouton number seen at the neuromuscular junction in shot3/shotVV third instar larvae.
Dendrites extend normally in shotunspecified mutant embryos expressing shotLA-Δrod1.Scer\UAS.T:Avic\GFP in motorneurons, but they appear less branched compared to wild-type.
Scer\GAL4sr-md710 driven expression of shotLA-Δrod1.Scer\UAS.T:Avic\GFP prevents muscle detachment in shotunspecified mutants, but 75% of tendon cells are abnormally elongated.
Expression of shotLA-Δrod1.Scer\UAS.T:Avic\GFP under the control of Scer\GAL41407 rescues the motor and sensory axon extension defects seen in shot3 mutants.