FlyBase Allele Report: Dmel\jing[UAS.cSa]

General Information

Symbol

Dmel\jing^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Sedaghat

FlyBase ID

FBal0138215

Feature type

allele

Associated gene

Dmel\jing

Associated Insertion(s)

Carried in Construct

P{UAS-jing.S}

Also Known As

P[UAS-jing]

Key Links

Transgenic product class

cDNA

(Sedaghat et al., 2002)

wild_type

(Sedaghat et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Sedaghat et al., 2002)

wild_type

(Sedaghat et al., 2002)

Mutagen

in vitro construct

(Sedaghat et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-jing.S}

Cytology

Description

UASt sequences drive expression of a jing cDNA.

(Sedaghat et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

jing

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^elav.PLu

(Sun et al., 2006)

abnormal neuroanatomy, with Scer\GAL4^sim.P3.7

(Sedaghat and Sonnenfeld, 2002)

abnormal neuroanatomy, with Scer\GAL4^sli.PS

(Sonnenfeld et al., 2004)

decreased cell death, with Scer\GAL4^sli.PS

(Sonnenfeld et al., 2004)

visible, with Scer\GAL4^GMR.PF

(Sonnenfeld et al., 2004)

Phenotype Manifest In

adult visceral tracheal branch, with Scer\GAL4^btl.PS

(Sedaghat et al., 2002)

embryonic/larval dorsal tracheal branch, with Scer\GAL4^btl.PS

(Sedaghat et al., 2002)

embryonic brain, with Scer\GAL4^sim.P3.7

(Sedaghat and Sonnenfeld, 2002)

eye, with Scer\GAL4^GMR.PF

(Sonnenfeld et al., 2004)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Sonnenfeld et al., 2004)

larval anterior commissure, with Scer\GAL4^elav.PLu

(Sun et al., 2006)

larval longitudinal connective, with Scer\GAL4^elav.PLu

(Sun et al., 2006)

larval longitudinal connective, with Scer\GAL4^sim.P3.7

(Sedaghat et al., 2002)

larval posterior commissure, with Scer\GAL4^elav.PLu

(Sun et al., 2006)

larval ventral midline glial cell | ectopic, with Scer\GAL4^sli.PS

(Sonnenfeld et al., 2004)

larval ventral nerve cord commissure, with Scer\GAL4^sli.PS

(Sonnenfeld et al., 2004)

ommatidium, with Scer\GAL4^GMR.PF

(Sonnenfeld et al., 2004)

symmetrical commissure, with Scer\GAL4^sim.P3.7

(Sedaghat et al., 2002)

tracheal dorsal trunk primordium, with Scer\GAL4^btl.PS

(Sedaghat et al., 2002)

tracheal transverse connective primordium, with Scer\GAL4^btl.PS

(Sedaghat et al., 2002)

Detailed Description

Statement

Reference

Expression of one copy of jing^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PF has no visible effects on ommatidial formation.

Expression of jing^Scer\UAS.cSa under the control of Scer\GAL4^elav.PLu results in thinner longitudinal connectives than normal and reduced spacing between the anterior and posterior commissures.

(Sun et al., 2006)

Expression of jing^Scer\UAS.cSa under the control of Scer\GAL4^sli.PS results in an increase in the number of midline glial cells compared to wild type, with an average of 12 glia per segment that are scattered laterally from the midline compared to 8 in wild-type embryos. The extra glia are still located dorsally to the ventral nerve cord. There is no cell death present in the glial population in these embryos, in contrast to the small apoptotic glia found surrounding the nerve cord in wild-type embryos. Commissural axons do not properly approach the midline in embryos expressing jing^Scer\UAS.cSa under the control of Scer\GAL4^sli.PS and instead appear stalled along the longitudinal tracts. The extra glia present in these embryos appear to migrate towards the longitudinal tracts. Expression of jing^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PF results in flies with a rough eye phenotype, consisting of highly disorganised ommatidia and mechanosensory bristles in 45% of flies. In addition the number of ommatidia is reduced by 50% compared to wild type.

(Sonnenfeld et al., 2004)

Expression of jing^Scer\UAS.cSa under the control of Scer\GAL4^sim.P3.7 inhibits the formation of the preoral and postoral tritocerebral commissures in the embryonic brain. The circumesophageal longitudinal connectives sometimes do not extend properly and have large gaps.

(Sedaghat and Sonnenfeld, 2002)

When jing^Scer\UAS.cSa is driven by Scer\GAL4^sim.P3.7, commissural and longitudinal axon formation is inhibited. When jing^Scer\UAS.cSa is driven by Scer\GAL4^btl.PS, defects are seen in dorsal trunk fusion, as well as improper formation of the transverse connective, dorsal branch and visceral branch.

(Sedaghat et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^elav.PLu, jing^UAS.cSa has abnormal neuroanatomy phenotype, enhanceable by XNP^EP635/Scer\GAL4^elav.PLu

(Sun et al., 2006)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, jing^UAS.cSa has visible phenotype, suppressible by Df(2L)TW50/+

(Sonnenfeld et al., 2004)

Scer\GAL4^GMR.PF, jing^UAS.cSa has visible phenotype, suppressible by Df(2R)Egfr5/+

(Sonnenfeld et al., 2004)

Scer\GAL4^GMR.PF, jing^UAS.cSa has visible phenotype, suppressible by spi¹/spi[+]

(Sonnenfeld et al., 2004)

Enhancer of

Statement

Reference

jing^UAS.cSa/Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, kay^EP3084

(Sun et al., 2006)

jing^UAS.cSa/Scer\GAL4^elav.PLu is an enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^elav.PLu, XNP^EP635

(Sun et al., 2006)

jing^UAS.cSa/Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, lap^EP3060

(Sun et al., 2006)

NOT Enhancer of

Statement

Reference

jing^UAS.cSa, Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Egfr^Elp.UAS, Scer\GAL4^GMR.PF

(Sonnenfeld et al., 2004)

Other

Statement

Reference

BicDR^EP3705, Scer\GAL4^GMR.PF, jing^UAS.cSa has visible phenotype

(Sun et al., 2006)

Scer\GAL4^GMR.PF, jigr1^EP3354, jing^UAS.cSa has visible phenotype

(Sun et al., 2006)

D1^EP473, Scer\GAL4^GMR.PF, jing^UAS.cSa has visible phenotype

(Sun et al., 2006)

Scer\GAL4^GMR.PF, XNP^EP635, jing^UAS.cSa has visible phenotype

(Sun et al., 2006)

Atx2^EP3145, Scer\GAL4^GMR.PF, jing^UAS.cSa has visible phenotype

(Sun et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^elav.PLu, jing^UAS.cSa has larval longitudinal connective phenotype, enhanceable by XNP^EP635/Scer\GAL4^elav.PLu

(Sun et al., 2006)

Suppressed by

Statement

Reference

jing^UAS.cSa has ommatidium phenotype, suppressible by Df(2R)Egfr5/+

(Sonnenfeld et al., 2004)

jing^UAS.cSa has ommatidium phenotype, suppressible by spi¹/spi[+]

(Sonnenfeld et al., 2004)

Scer\GAL4^sli.PS, jing^UAS.cSa has larval ventral midline glial cell | ectopic phenotype, suppressible by S^IIN/S^IIN

(Sonnenfeld et al., 2004)

Scer\GAL4^sli.PS, jing^UAS.cSa has larval ventral midline glial cell | ectopic phenotype, suppressible by spi¹/spi¹

(Sonnenfeld et al., 2004)

jing^UAS.cSa has ommatidium phenotype, suppressible by Df(2L)TW50/+

(Sonnenfeld et al., 2004)

NOT suppressed by

Statement

Reference

Scer\GAL4^sli.PS, jing^UAS.cSa has larval ventral midline glial cell | ectopic phenotype, non-suppressible by S[+]/S^IIN

(Sonnenfeld et al., 2004)

Scer\GAL4^sli.PS, jing^UAS.cSa has larval ventral midline glial cell | ectopic phenotype, non-suppressible by spi¹/spi[+]

(Sonnenfeld et al., 2004)

Enhancer of

Statement

Reference

jing^UAS.cSa/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, kay^EP3084

(Sun et al., 2006)

jing^UAS.cSa/Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, kay^EP3084

(Sun et al., 2006)

jing^UAS.cSa/Scer\GAL4^GMR.PF is an enhancer of pigment cell phenotype of Scer\GAL4^GMR.PF, kay^EP3084

(Sun et al., 2006)

jing^UAS.cSa/Scer\GAL4^elav.PLu is an enhancer of larval longitudinal connective phenotype of Scer\GAL4^elav.PLu, XNP^EP635

(Sun et al., 2006)

jing^UAS.cSa/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, lap^EP3060

(Sun et al., 2006)

jing^UAS.cSa/Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, lap^EP3060

(Sun et al., 2006)

NOT Enhancer of

Statement

Reference

jing^UAS.cSa, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Egfr^Elp.UAS, Scer\GAL4^GMR.PF

(Sonnenfeld et al., 2004)

jing^UAS.cSa, Scer\GAL4^GMR.PF is a non-enhancer of ommatidium phenotype of Egfr^Elp.UAS, Scer\GAL4^GMR.PF

(Sonnenfeld et al., 2004)

NOT Suppressor of

Statement

Reference

jing^UAS.cSa/Scer\GAL4^sli.PS is a non-suppressor of larval ventral midline glial cell phenotype of S^IIN

(Sonnenfeld et al., 2004)

jing^UAS.cSa/Scer\GAL4^sli.PS is a non-suppressor of larval ventral midline glial cell phenotype of spi¹

(Sonnenfeld et al., 2004)

Other

Statement

Reference

D1^EP473, Scer\GAL4^GMR.PF, jing^UAS.cSa has eye phenotype

(Sun et al., 2006)

D1^EP473, Scer\GAL4^GMR.PF, jing^UAS.cSa has ommatidium phenotype

(Sun et al., 2006)

BicDR^EP3705, Scer\GAL4^GMR.PF, jing^UAS.cSa has eye phenotype

(Sun et al., 2006)

BicDR^EP3705, Scer\GAL4^GMR.PF, jing^UAS.cSa has ommatidium phenotype

(Sun et al., 2006)

Scer\GAL4^GMR.PF, jigr1^EP3354, jing^UAS.cSa has eye phenotype

(Sun et al., 2006)

Scer\GAL4^GMR.PF, jigr1^EP3354, jing^UAS.cSa has ommatidium phenotype

(Sun et al., 2006)

Scer\GAL4^GMR.PF, XNP^EP635, jing^UAS.cSa has eye phenotype

(Sun et al., 2006)

Scer\GAL4^GMR.PF, XNP^EP635, jing^UAS.cSa has ommatidium phenotype

(Sun et al., 2006)

Atx2^EP3145, Scer\GAL4^GMR.PF, jing^UAS.cSa has eye phenotype

(Sun et al., 2006)

Atx2^EP3145, Scer\GAL4^GMR.PF, jing^UAS.cSa has ommatidium phenotype

(Sun et al., 2006)

Scer\GAL4^GMR.PF, jing^UAS.cSa, lap^EP3060 has pigment cell phenotype

(Sun et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of jing^Scer\UAS.cSa enhances the severity of the rough eye phenotype caused by expression of lap^EP3060 under the control of Scer\GAL4^GMR.PF and in addition, loss of pigmentation in the eye is seen.

Co-expression of jing^Scer\UAS.cSa and XNP^EP635 under the control of Scer\GAL4^elav.PLu results in synergistic effects in the embryonic central nervous system; 65% of segments have no longitudinal connectives.

(Sun et al., 2006)

The increase in the number of midline glia per ventral nerve cord segment seen in embryos expressing jing^Scer\UAS.cSa under the control of Scer\GAL4^sli.PS is not suppressed by spi¹, but expression of jing^Scer\UAS.cSa under the control of Scer\GAL4^sli.PS in a homozygous spi¹ background does not induce extra midline glia and the number of midline glia per ventral nerve cord segment in these double mutant embryos is reduced compared to wild type and is similar to that seen in homozygous spi¹ single mutants. The rough eye phenotype caused by expression of Egfr^Elp.Scer\UAS under the control of Scer\GAL4^GMR.PF is not enhanced by coexpression of jing^Scer\UAS.cSa. The rough eye phenotype caused by expression of jing^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PF is dominantly suppressed by spi¹, Df(2L)TW50 or Df(2R)Egfr5.

(Sonnenfeld et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

jing^Scer\UAS.cSa

jing^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Sedaghat

(Sedaghat et al., 2002)

Secondary FlyBase IDs

References (4)

Report Sections

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