FB2026_02 , released June 18, 2026
Allele: Dmel\spinΔ2b
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General Information
Symbol
Dmel\spinΔ2b
Species
D. melanogaster
Name
FlyBase ID
FBal0143208
Feature type
allele
Associated gene
Dmel\spin
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Dermaut et al., 2005, Sweeney and Davis, 2002)
Mutagen
Delta2-3 transposase
(Sweeney and Davis, 2002)
Progenitor genotype
P{EP}spinEP822
(Sweeney and Davis, 2002)
Cytology
Description

Excision of the P{EP} element resulting in a deletion of approximately 5kb from the spin translation start site, removing all the coding sequences within the first exon (includes the N-terminal and first transmembrane domain).

(Sweeney and Davis, 2002)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  tauopathy
      modeled by Hsap\MAPTGMR.Ex.PJ
      (Dermaut et al., 2005)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal locomotor behavior (with spinE14.1)

      (Dermaut et al., 2005)

      abnormal locomotor behavior (with spinN)

      (Dermaut et al., 2005)

      abnormal locomotor behavior (with spinP)

      (Dermaut et al., 2005)

      abnormal locomotor behavior (with spinΔ29)

      (Dermaut et al., 2005)

      abnormal locomotor behavior (with spinΔ31)

      (Dermaut et al., 2005)

      abnormal locomotor behavior (with spinΔ86)

      (Dermaut et al., 2005)

      abnormal locomotor behavior | recessive

      (Dermaut et al., 2005)

      abnormal neuroanatomy (with Df(2R)Jp4)

      (Sweeney and Davis, 2002)

      abnormal neuroanatomy | dominant

      (Sweeney and Davis, 2002)

      abnormal neurophysiology (with Df(2R)Jp4)

      (Sweeney and Davis, 2002)

      abnormal neurophysiology | larval stage (with spin11F5)

      (Dermaut et al., 2005)

      abnormal neurophysiology | larval stage (with spinE14.1)

      (Dermaut et al., 2005)

      lethal (with Df(2R)Jp8)

      (Dermaut et al., 2005)

      lethal (with spin11F5)

      (Dermaut et al., 2005)

      lethal (with spinE14.1)

      (Dermaut et al., 2005)

      lethal (with spinN)

      (Dermaut et al., 2005)

      lethal (with spinP)

      (Dermaut et al., 2005)

      lethal (with spinΔ29)

      (Dermaut et al., 2005)

      lethal (with spinΔ31)

      (Dermaut et al., 2005)

      lethal (with spinΔ86)

      (Dermaut et al., 2005)

      lethal - all die before end of pupal stage (with spin10403)

      (Sweeney and Davis, 2002)

      lethal - all die before end of pupal stage (with spinEP822)

      (Sweeney and Davis, 2002)

      lethal - all die before end of pupal stage (with spink09905)

      (Sweeney and Davis, 2002)

      lethal - all die before end of pupal stage | recessive

      (Sweeney and Davis, 2002)

      short lived (with spinE14.1)

      (Dermaut et al., 2005)

      short lived (with spinN)

      (Dermaut et al., 2005)

      short lived (with spinP)

      (Dermaut et al., 2005)

      short lived (with spinΔ29)

      (Dermaut et al., 2005)

      short lived (with spinΔ31)

      (Dermaut et al., 2005)

      short lived (with spinΔ86)

      (Dermaut et al., 2005)

      short lived | recessive

      (Dermaut et al., 2005)

      some die during pupal stage (with spin10403)

      (Sweeney and Davis, 2002)

      some die during pupal stage (with spinEP822)

      (Sweeney and Davis, 2002)

      some die during pupal stage (with spink09905)

      (Sweeney and Davis, 2002)

      some die during pupal stage | recessive

      (Sweeney and Davis, 2002)
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The following transheterozygotes show small amounts of adult escapers, depending on culture conditions: spinΔ2b/spinE14.1, spinΔ29/spinΔ2b, spinΔ31/spinΔ2b, spinΔ86/spinΔ2b and spinΔ2b/spinΔ2b. Adult escapers exhibit progressive locomotor defects, such as difficulty in righting after a fall. These defects worsen during the days after emergence and result in death within 5-12 days. The escapers appear morphologically normal except for a subtle, completely penetrant extra wing vein phenotype.

      Oocytes produced from spinΔ2b germline clones show dramatically enlarged carbohydrate-containing β spheres in the ooplasm, while protein-containing α spheres are similar in size to wild-type oocytes.

      The larval NMJ of both spinE14.1/spinΔ2b and spin11F5/spinΔ2b mutants contain abnormal ultrastructural membrane compartments in the cytoplasm of bnch mutant boutons that are not present in wild-type controls. The excitatory junctional potentials (EJPs) produced by abdominal muscles in 1mM ca2+ from spinE14.1/spinΔ2b and spin11F5/spinΔ2b mutants are not significantly different from those produced by wild-type, indicating that exocytosis is not affected in mutants. When motor neurons from the mutants are repetitively stimulated at 10Hz, the amplitude of the EJP measured from spinE14.1/spinΔ2b and spin11F5/spinΔ2b mutants declines to 55-60% of the original response after 10 minutes, while no such decline is observed for wild-type animals. Additionally, spinE14.1/spinΔ2b mutant boutons show a significant decrease in the uptake of FM1-43 dye compared with controls. These results indicate that the mutants have a defect in synaptic vesicle endocytosis.

      Mosaic spinΔ2b eyes show no obvious rough eye phenotypes.

      (Dermaut et al., 2005)

      Mutation is lethal at the pharate pupal stage. Heterozygotes and spinΔ2b/Df(2R)Jp4 hemizygotes show a significant increase in bouton number at the larval neuromuscular junction (assayed at muscles 6/7 in hemisegment A3) compared to wild type. Average bouton size spinΔ2b/Df(2R)Jp4 animals is not significantly different from wild type. Quantal content at the neuromuscular junction is decreased by approximately 50% compared to wild type in spinΔ2b/Df(2R)Jp4 animals. Quantal size is unaffected.

      (Sweeney and Davis, 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Other
      Statement
      Reference

      Hsap\MAPTGMR.Ex.PJ, spinΔ2b has eye phenotype

      (Dermaut et al., 2005)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Flies that express Hsap\MAPTGMR.Ex.PJ in spinΔ2b/+ mosaic eyes show a mild rough eye phenotype while flies that express Hsap\MAPTGMR.Ex.PJ in spinΔ2b homozygous mosaic eyes show a severe rough eye phenotype.

      (Dermaut et al., 2005)
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      Df spinΔ2b
      (Sweeney and Davis, 2002)
      bnchΔ2B
      (Dermaut et al., 2005)
      bnchΔ2b
      (Dermaut et al., 2005)
      spinΔ2B
      (Sweeney and Davis, 2002)
      spinΔ2b
      (Yuva-Aydemir et al., 2011)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)