FlyBase Allele Report: Dmel\numb[PTB.UAS.GFP]

General Information

Symbol

Dmel\numb^PTB.UAS.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0146968

Feature type

allele

Associated gene

Dmel\numb

Associated Insertion(s)

Carried in Construct

P{UAS-numb.PTB.GFP}

Also Known As

UAS-numb-gfp

Key Links

Transgenic product class

inframe_deletion

(Roegiers et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Roegiers et al., 2001)

Mutagen

in vitro construct

(Roegiers et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-numb.PTB.GFP}

Cytology

Description

UAS regulatory sequences drive expression of the PTB domain of numb tagged with GFP.

(Roegiers et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

numb

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

ameliorates brain cancer

modeled by aurA⁸⁸³⁹

(Wang et al., 2006)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

external sensory organ precursor cell IIa, with Scer\GAL4^{neur-GAL4-A101}

(Justice et al., 2003)

external sensory organ precursor cell IIb | increased number, with Scer\GAL4^{neur-GAL4-A101}

(Justice et al., 2003)

tormogen cell, with Scer\GAL4^{neur-GAL4-A101}

(Justice et al., 2003)

trichogen cell | increased number, with Scer\GAL4^{neur-GAL4-A101}

(Justice et al., 2003)

Detailed Description

Statement

Reference

Overexpression of numb^{PTB.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^insc-Mz1407 show no effect on neuroblast maintenance.

(Song and Lu, 2012)

Expression of numb^{PTB.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^wor.PA in a wild-type background does not affect neuroblast number in the larval central brain.

(Wang et al., 2007)

When numb^{PTB.Scer\UAS.T:Avic\GFP} is driven by Scer\GAL4^{neur-GAL4-A101} balding and twinned hairs are seen on the notum due to the transformation of pIIa to pIIb and transformation of socket to hair.

(Justice et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

numb^PTB.UAS.GFP, Scer\GAL4^GMR.PF is an enhancer of visible | adult stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407/numb^PTB.UAS.GFP is a suppressor of abnormal neuroanatomy | larval stage phenotype of mts²⁹⁹/mts^s5286

(Wang et al., 2009)

Scer\GAL4^insc-Mz1407/numb^PTB.UAS.GFP is a suppressor of increased cell number phenotype of mts²⁹⁹/mts^s5286

(Wang et al., 2009)

Scer\GAL4^wor.PA/numb^PTB.UAS.GFP is a suppressor of hyperplasia | somatic clone | larval stage phenotype of polo^S025604

(Wang et al., 2007)

Scer\GAL4^wor.PA/numb^PTB.UAS.GFP is a suppressor of abnormal neuroanatomy phenotype of aurA⁸⁸³⁹

(Wang et al., 2006)

Phenotype Manifest In

Enhancer of

Statement

Reference

numb^PTB.UAS.GFP, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407/numb^PTB.UAS.GFP is a suppressor of neuroblast | increased number phenotype of mts²⁹⁹/mts^s5286

(Wang et al., 2009)

Scer\GAL4^insc-Mz1407/numb^PTB.UAS.GFP is a suppressor of larval brain phenotype of mts²⁹⁹/mts^s5286

(Wang et al., 2009)

Scer\GAL4^wor.PA/numb^PTB.UAS.GFP is a suppressor of neuroblast & larval brain | somatic clone phenotype of polo^S025604

(Wang et al., 2007)

Scer\GAL4^wor.PA/numb^PTB.UAS.GFP is a suppressor of neuroblast | supernumerary & larval brain phenotype of aurA⁸⁸³⁹

(Wang et al., 2006)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{neur-GAL4-A101}/numb^PTB.UAS.GFP is a non-suppressor of eo neuron | somatic clone phenotype of l(2)gl^unspecified

(Justice et al., 2003)

Scer\GAL4^{neur-GAL4-A101}/numb^PTB.UAS.GFP is a non-suppressor of thecogen cell | somatic clone phenotype of l(2)gl^unspecified

(Justice et al., 2003)

Scer\GAL4^{neur-GAL4-A101}/numb^PTB.UAS.GFP is a non-suppressor of tormogen cell | somatic clone phenotype of l(2)gl^unspecified

(Justice et al., 2003)

Scer\GAL4^{neur-GAL4-A101}/numb^PTB.UAS.GFP is a non-suppressor of tormogen cell | increased number | somatic clone phenotype of l(2)gl^unspecified

(Justice et al., 2003)

Scer\GAL4^{neur-GAL4-A101}/numb^PTB.UAS.GFP is a non-suppressor of trichogen cell | somatic clone phenotype of l(2)gl^unspecified

(Justice et al., 2003)

Scer\GAL4^{neur-GAL4-A101}/numb^PTB.UAS.GFP is a non-suppressor of trichogen cell | increased number | somatic clone phenotype of l(2)gl^unspecified

(Justice et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

External sensory organ cells that are expressing numb^{PTB.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^tub and are also homozygous for seq^A41 acquire a socket fate.

(Andrews et al., 2009)

The ectopic neuroblast self-renewal phenotype seen in single cell polo^S025604 clones in the larval central brain is suppressed by expression of numb^{PTB.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^wor.PA.

(Wang et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^{neur-GAL4-A101}/numb^PTB.UAS.GFP partially rescues numb²

(Justice et al., 2003)

Comments

The expression of numb^{PTB.Scer\UAS.T:Avic\GFP} driven by Scer\GAL4^{neur-GAL4-A101} rescues the multiple socket phenotype, seen in numb² clones, in 60% of mutant external sensory organs.

(Justice et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

51663

w^*; P{UAS-numb.PTB.GFP}3

51662

w^*; P{UAS-numb.PTB.GFP}2/CyO

Notes on Origin

Discoverer