FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\foxoTM.UAS
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General Information
Symbol
Dmel\foxoTM.UAS
Species
D. melanogaster
Name
triple mutant
FlyBase ID
FBal0151925
Feature type
allele
Associated gene
Dmel\foxo
Associated Insertion(s)
Carried in Construct
P{UAS-foxo.TM}
Also Known As
UAS-FoxO-TM, foxoTM, UAS-dFOXO-TM, UAS-FOXOTM
Key Links
Transgenic product class
missense_variant
(Hwangbo et al., 2004)
polypeptide_post_translational_processing_variant
(Hwangbo et al., 2004)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Hwangbo et al., 2004)
polypeptide_post_translational_processing_variant
(Hwangbo et al., 2004)
Mutagen
in vitro construct
(Hwangbo et al., 2004, Junger et al., 2003)
Progenitor genotype
foxoUAS.cFa
(Hwangbo et al., 2004, Junger et al., 2003)
Carried in construct
P{UAS-foxo.TM}
Cytology
Description

The three Akt1 phosphorylation sites are mutated (amino acid replacements T44A, S190A and S259A).

(Hwangbo et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
foxo
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Motor neuronal overexpression of foxoTM.Scer\UAS (under the control of Scer\GAL4Rapgap1-OK6 produces third instar larvae that are reduced in size. Normalised to muscle area, bouton number in this background does not differ from wild-type. However, the size of individual synaptic boutons is increased. Using futsch staining as a marker, microtubule stability is also reduced in these mutants.

      (Nechipurenko and Broihier, 2012)

      Overexpression of foxoTM.Scer\UAS in adult muscles under the control of Scer\GAL4Mef2.PR and Scer\GAL80ts.αTub84B significantly preserves muscle protein homeostasis, while the muscles of wild-type animals display increased accumulation of protein aggregates.

      (Demontis and Perrimon, 2010)

      Expression of foxoTM.Scer\UAS under the control of Scer\GAL4Act5C.PP results in clones in the fat body results in a strong induction of autophagy.

      (Juhász et al., 2007)

      When foxoTM.Scer\UAS is driven by Scer\GAL4bun-Switch1.32 (in adults fed on mifepristone) the median lifespan can increased by as much as 56%. Fecundity is unaffected.

      (Hwangbo et al., 2004)

      Flies expressing foxoTM.Scer\UAS under the control of both Scer\GAL4tin.cBa and Scer\GAL4Scer\UAS.cHa have a higher heart rate than wild-type flies; this decreases slightly with age but to a much lesser extant than in wild type. Additionally, stress-induced heart failure rate does not increase with age in these flies, in contrast to the increase seen in wild-type flies.

      (Wessells et al., 2004)

      Appears to cause lethality even in absence of Scer\GAL4 driver.

      (Junger et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of foxoTM.Scer\UAS partially suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU.

      (Read et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      foxoTM.Scer\UAS
      foxoTM.UAS
      foxoTM
      (Becker et al., 2010)
      Name Synonyms
      triple mutant
      (Junger et al., 2003)
      Secondary FlyBase IDs
        References (18)