Motor neuronal overexpression of foxoTM.Scer\UAS (under the control of Scer\GAL4Rapgap1-OK6 produces third instar larvae that are reduced in size. Normalised to muscle area, bouton number in this background does not differ from wild-type. However, the size of individual synaptic boutons is increased. Using futsch staining as a marker, microtubule stability is also reduced in these mutants.
Overexpression of foxoTM.Scer\UAS in adult muscles under the control of Scer\GAL4Mef2.PR and Scer\GAL80ts.αTub84B significantly preserves muscle protein homeostasis, while the muscles of wild-type animals display increased accumulation of protein aggregates.
Flies expressing foxoTM.Scer\UAS under the control of both Scer\GAL4tin.cBa and Scer\GAL4Scer\UAS.cHa have a higher heart rate than wild-type flies; this decreases slightly with age but to a much lesser extant than in wild type. Additionally, stress-induced heart failure rate does not increase with age in these flies, in contrast to the increase seen in wild-type flies.
Appears to cause lethality even in absence of Scer\GAL4 driver.