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General Information
Symbol
Dmel\foxoTM.UAS
Species
D. melanogaster
Name
triple mutant
FlyBase ID
FBal0151925
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
foxoTM, UAS-dFOXO-TM, UAS-FoxO-TM
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The three Akt1 phosphorylation sites are mutated (amino acid replacements T44A, S190A and S259A).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Motor neuronal overexpression of foxoTM.Scer\UAS (under the control of Scer\GAL4Rapgap1-OK6 produces third instar larvae that are reduced in size. Normalised to muscle area, bouton number in this background does not differ from wild-type. However, the size of individual synaptic boutons is increased. Using futsch staining as a marker, microtubule stability is also reduced in these mutants.

Overexpression of foxoTM.Scer\UAS in adult muscles under the control of Scer\GAL4Mef2.PR and Scer\GAL80ts.αTub84B significantly preserves muscle protein homeostasis, while the muscles of wild-type animals display increased accumulation of protein aggregates.

Expression of foxoTM.Scer\UAS under the control of Scer\GAL4Act5C.PP results in clones in the fat body results in a strong induction of autophagy.

When foxoTM.Scer\UAS is driven by Scer\GAL4bun-Switch1.32 (in adults fed on mifepristone) the median lifespan can increased by as much as 56%. Fecundity is unaffected.

Flies expressing foxoTM.Scer\UAS under the control of both Scer\GAL4tin.cBa and Scer\GAL4Scer\UAS.cHa have a higher heart rate than wild-type flies; this decreases slightly with age but to a much lesser extant than in wild type. Additionally, stress-induced heart failure rate does not increase with age in these flies, in contrast to the increase seen in wild-type flies.

Appears to cause lethality even in absence of Scer\GAL4 driver.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Expression of foxoTM.Scer\UAS partially suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
foxoTM.Scer\UAS
foxoTM.UAS
Name Synonyms
triple mutant
Secondary FlyBase IDs
    References (14)