FlyBase Allele Report: Dmel\Act5C[G0010]

General Information

Symbol

Dmel\Act5C^G0010

Species

D. melanogaster

Name

FlyBase ID

FBal0152800

Feature type

allele

Associated gene

Dmel\Act5C

Associated Insertion(s)

P{lacW}Act5C^G0010

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Schäfer et al., 1999.12.2)

Progenitor genotype

Associated Insertion(s)

P{lacW}Act5C^G0010

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:5,902,157..5,902,289 [+]

(Hsiao et al., 2014, Peter et al., 2002, Schäfer et al., 1999.12.2)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

AJ426702

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates synucleinopathy

modeled by Hsap\SNCA^QUAS.cOa

(Ordonez et al., 2018)

ameliorates tauopathy

modeled by Hsap\MAPT^V337M.UAS

(Fulga et al., 2007)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Schäfer et al., 1999.12.2)

lethal - all die before end of larval stage

(Peter et al., 2002)

some die during larval stage

(Peter et al., 2002)

Phenotype Manifest In

Detailed Description

Statement

Reference

Act5C^G0010 heterozygosity has no significant effect on the number of neurons or on the mitochondria size in the adult brain compared to controls.

(Ordonez et al., 2018)

Lethality occurs during the first and second larval instars.

(Peter et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Act5C^G0010/Act5C[+] is a suppressor of abnormal locomotor behavior | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

Act5C^G0010/Act5C[+] is a suppressor of decreased cell number | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

Act5C^G0010/Act5C[+] is a suppressor of abnormal size | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

Phenotype Manifest In

Enhancer of

Statement

Reference

Act5C^G0010/Act5C[+] is an enhancer of eye phenotype of Hsap\MAPT^E14.UAS, Scer\GAL4^GMR.PF

(Fulga et al., 2007)

NOT Enhancer of

Statement

Reference

Act5C^G0010/Act5C[+] is a non-enhancer of adult head bristle phenotype of sc^10-1

(Hsiao et al., 2014)

Suppressor of

Statement

Reference

Act5C^G0010/Act5C[+] is a suppressor of adult brain phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

Act5C^G0010/Act5C[+] is a suppressor of neuron | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

Act5C^G0010/Act5C[+] is a suppressor of dopaminergic medulla neuron phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

Act5C^G0010/Act5C[+] is a suppressor of filamentous actin | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

Act5C^G0010/Act5C[+] is a suppressor of mitochondrion | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

Act5C^G0010/Act5C[+] is a suppressor of eye phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Fulga et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The percentage of the double heterozygous sc^10-1/+; +/+; Act5C^G0010/+ flies missing post-vertical head bristles are no significantly different or only mildly increased compared with sc^10-1/+ flies.

(Hsiao et al., 2014)

Xenogenetic Interactions

Statement

Reference

The adult locomotor activity defects, the adult brain vacuolization and the decreased number of neurons (including TH-immunoreactive dopaminergic neurons) and the increased number of rod-shaped actin-rich inclusions in the adult brain resulting from the expression of Hsap\SNCA^{Ncra\QUAS.cOa} under the control of Ncra\QF^QF2.nSyb are partially suppressed by Act5C^G0010 heterozygosity.

The increased mitochondria size in the adult brain of individuals expressing Hsap\SNCA^{Ncra\QUAS.cOa} under the control of Ncra\QF^QF2.nSyb is partially suppressed by Act5C^G0010 heterozygosity.

(Ordonez et al., 2018)

The rough-eye phenotype observed in flies expressing Hsap\MAPT^{V337M.Scer\UAS} in the eye under the control of Scer\GAL4^GMR.PF is suppressed in a Act5C^G0010/+ background.

The rough-eye phenotype observed in animals expressing Hsap\MAPT^E14.Scer\UAS in the retina under the control of Scer\GAL4^GMR.PF is enhanced in a Act5C^G0010/+ background.

(Fulga et al., 2007)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

12083

w^67c23 P{lacW}Act5C^G0010/FM7c

Kyoto

111909

w^67c23 P{lacW}Act5C^G0010 P{neoFRT}19A/FM7c; P{ey-FLP.N}5

Notes on Origin

Discoverer

Comments

P{lacW} not verified as causing the lethality.

(Schäfer et al., 1999.12.2)

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AJ426702

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Act5C^G0010

(Ordonez et al., 2018, Hsiao et al., 2014, Nie et al., 2014)

act5C^G0010

(Fulga et al., 2007)

act5C^cg0010

(Hong and Choi, 2016)

l(1)G0010

(Peter et al., 2002)

l(1)G0010^G0010

(Schäfer et al., 1999.12.2)

Name Synonyms

Secondary FlyBase IDs

FBal0103775

References (8)

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