FlyBase Allele Report: Dmel\shep[BG00836]

General Information

Symbol

Dmel\shep^BG00836

Species

D. melanogaster

Name

FlyBase ID

FBal0157046

Feature type

allele

Associated gene

Dmel\shep

Associated Insertion(s)

P{GT1}shep^BG00836

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(FlyBase, 1996-)

Progenitor genotype

Associated Insertion(s)

P{GT1}shep^BG00836

Cytology

Description

The P{GT1}CG10677^BG00836 insertion maps within both CG10677 and alan-shepard (nested genes).

(FlyBase, 1996-)

Allele components

Component

Use(s)

Inserted element

P{GT1}

gene trap

polyA trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:5,184,769..5,184,769 [+]

(Olesnicky et al., 2018, Schachtner et al., 2015, Chen et al., 2014, FlyBase, 1996-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult CCAP neuron | pharate adult stage

(Chen et al., 2019)

axon | pharate adult stage (with Df(3L)ED210)

(Chen et al., 2014)

neuronal cell body | pharate adult stage (with Df(3L)ED210)

(Chen et al., 2014)

NMJ bouton | pharate adult stage (with Df(3L)ED210)

(Chen et al., 2014)

peptidergic neuron | pharate adult stage (with Df(3L)ED210)

(Chen et al., 2014)

wing

(Chen et al., 2014)

wing vein | ectopic

(Chen et al., 2014)

Detailed Description

Statement

Reference

shep^BG00836/shep^BG00836 Burs-positive CCAP neurons have smaller cell bodies and fewer projections than heterozygous controls in pharate adults.

(Chen et al., 2019)

shep^BG00836/Df(3L)ED210 mutant females display defects in courtship and mating behaviors. When crossed to wild type males the females vigorously kick at the males during mounting and copulation, and copulation duration is reduced compared to controls. Copulation latency (time taken to begin copulation) is unchanged from controls. In contrast to wild type, shep^BG00836/Df(3L)ED210 females display extensive three-legged (compared to the normal two-legged) grooming and expulsion of seminal fluids from the reproductive tract after copulation.

At the P14 pharate adult stage, the bursicon neuron somata in shep^BG00836/Df(3L)ED210 mutant animals have lost the multiangular morphology characteristic of that stage and become more rounded. Soma area and peripheral axon branching are reduced. The peripheral bursicon axons also display fewer boutons than controls. Bursicon neuron soma area is unchanged in shep^BG00836/Df(3L)ED210 larvae compared to controls and there is no change in the number and size distribution of NMJ boutons.

At the P14 pharate adult stage, the bursicon neuron somata in homozygous shep^BG00836 mutant animals are reduced in area. Bursicon neuron soma area is unchanged in shep^BG00836/Df(3L)ED210 larvae compared to controls.

(Chen et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

shep^BG00836 is rescued by Scer\GAL4^Ser.PGF/shep^UAS.E

(Chen et al., 2019)

shep^BG00836/Df(3L)ED210 is rescued by Scer\GAL4^BG00836/shep^UAS.EG

(Chen et al., 2014)

Partially rescued by

shep^BG00836/Df(3L)ED210 is partially rescued by Scer\GAL4^BG00836/shep^UAS.EG

(Chen et al., 2014)

Not rescued by

shep^BG00836 is not rescued by shep^RRM.UAS.E/Scer\GAL4^Ser.PGF

(Chen et al., 2019)

Comments

Expression of shep^Scer\UAS.EG under the control of Scer\GAL4^BG00836 partially suppresses the kicking exhibited by shep^BG00836/Df(3L)ED210 mutant females during mounting and copulation. The copulation duration is restored to wild type levels. Expression of shep^Scer\UAS.EG does not rescue the grooming defects seen after copulation.

Expression of shep^Scer\UAS.EG under the control of Scer\GAL4^BG00836 fully rescues the bursicon neuron soma area, peripheral axon branch number and bouton number defects seen in shep^BG00836/Df(3L)ED210 mutant pharate adults.

(Chen et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

12513

w¹¹¹⁸; P{GT1}shep^BG00836

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG32423^BG00836

alan-shepard^BG00836

shep^BG00836

(Chen et al., 2021, Chen et al., 2019, Olesnicky et al., 2018, Schachtner et al., 2015, Chen et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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