FlyBase Allele Report: Dmel\Fer2[e03248]

General Information

Symbol

Dmel\Fer2^e03248

Species

D. melanogaster

Name

FlyBase ID

FBal0160472

Feature type

allele

Associated gene

Dmel\Fer2

Associated Insertion(s)

PBac{RB}Fer2^e03248

Carried in Construct

Also Known As

Fer2¹

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

piggyBac activity

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Bou Dib et al., 2014, Nagoshi et al., 2010)

Mutagen

piggyBac activity

(Bloomington Drosophila Stock Center, 2004.11.6)

Progenitor genotype

Associated Insertion(s)

PBac{RB}Fer2^e03248

Cytology

Description

Allele components

Component

Use(s)

Inserted element

PBac{RB}

gene trap

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:16,151,329..16,151,329 [+]

(Miozzo et al., 2022, Bou Dib et al., 2014, Nagoshi et al., 2010, Bloomington Drosophila Stock Center, 2004.11.6)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult dopaminergic PAM neuron

(Bou Dib et al., 2014)

adult dopaminergic PAM neuron | adult stage | decreased number

(Miozzo et al., 2022)

adult s-LNv neuron

(Nagoshi et al., 2010)

l-LNv neuron

(Nagoshi et al., 2010)

LNd neuron

(Nagoshi et al., 2010)

mitochondrion

(Bou Dib et al., 2014)

Detailed Description

Statement

Reference

Fer2^e03248 homozygous adults show a decrease in climbing ability. They also show a decrease in the number of PAM neurons.

(Miozzo et al., 2022)

Fer2^e03248 mutant flies do not exhibit any obvious differences in the overall morphology of the cholinergic, glutamatergic and serotonergic neurons, but there is an evident reduction in dopaminergic neurons: progressive loss of the PAM and PAL cluster neurons is seen in homozygous Fer2^e03248 flies compared to controls. As in controls, PAM and PAL neuron number increases during the pupal stages, but never reaches wild type levels. Other dopaminergic neuron clusters are unaffected.

Homozygous Fer2^e03248 mutant flies exhibit climbing defects and these are partially but significantly rescued by feeding the flies L-Dopa. There is no obvious rescue of the dopaminergic neuron loss.

The majority of visible mitochondria in the cell bodies of remaining PAM neurons in Fer2^e03248 mutant flies are in enlarged aggregations and do not form tubular networks as in th control flies.

(Bou Dib et al., 2014)

Fer2^e03248 homozygotes, Fer2^e03248/Df(3R)sbd104 hemizygotes or Fer2^e03248/Df(3R)sbd45 hemizygotes show no rhythmic locomotor behaviour either in constant dark or light-dark conditions.

Homozygotes have significantly fewer LNv and LNd neurons than controls.

(Nagoshi et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Fer2^e03248 has abnormal neuroanatomy phenotype, suppressible | partially by Scer\GAL4^Fer2.2359/Cele\hlh-13^UAS.cDa

(Bou Dib et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Fer2^e03248 has adult dopaminergic PAM neuron phenotype, suppressible | partially by Scer\GAL4^Fer2.2359/Cele\hlh-13^UAS.cDa

(Bou Dib et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Cele\hlh-13^Scer\UAS.cDa under the control of Scer\GAL4^Fer2.2359 partially but significantly rescues the loss of dopaminergic PAM and PAL neurons in Fer2^e03248 mutant flies.

(Bou Dib et al., 2014)

Complementation and Rescue Data

Rescued by

Fer2^e03248 is rescued by Fer2^{fTRG00336.sfGFP-TVPTBF}

(Miozzo et al., 2022)

Partially rescued by

Fer2^e03248 is partially rescued by Fer2^UAS.Tag:FLAG/Scer\GAL4^Fer2.2359

(Bou Dib et al., 2014)

Not rescued by

Fer2^e03248 is not rescued by Fer2^UAS.Tag:FLAG/Scer\GAL4^GMR58E02

(Bou Dib et al., 2014)

Fer2^e03248 is not rescued by Scer\GAL4^Ddc.HL9/Fer2^UAS.Tag:FLAG

(Bou Dib et al., 2014)

Comments

Expression of Fer2^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Fer2.2359 partially but significantly rescues the climbing defects seen in Fer2^e03248 mutant flies. The PAM and PAL dopaminergic neuron loss is also rescued to quasi wild-type levels.

Expression of Fer2^{Scer\UAS.T:Zzzz\FLAG} in PAM neurons under the control of either Scer\GAL4^GMR58E02 or Scer\GAL4^Ddc.HL9 does not rescue the PAM neuron loss in Fer2^e03248 mutant flies.

(Bou Dib et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

86028

w¹¹¹⁸; PBac{RB}Fer2^e03248

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Fer2¹

(Miozzo et al., 2022, Bou Dib et al., 2014)

Fer2^e03248

(Bou Dib et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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