FlyBase Allele Report: Dmel\sra[UAS.cEa]

General Information

Symbol

Dmel\sra^UAS.cEa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Ejima

FlyBase ID

FBal0175434

Feature type

allele

Associated gene

Dmel\sra

Associated Insertion(s)

Carried in Construct

P{UAS-sra.E}

Also Known As

UAS-sra

Key Links

Transgenic product class

cDNA

(Ejima et al., 2004)

wild_type

(Ejima et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Ejima et al., 2004)

wild_type

(Ejima et al., 2004)

Mutagen

in vitro construct

(Ejima et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-sra.E}

Cytology

Description

UASt regulatory sequences drive expression of the sra LD15403 cDNA.

(Ejima et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

sra

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior | female, with Scer\GAL4^elav-C155

(Ejima et al., 2004)

abnormal courtship behavior | female, with Scer\GAL4^sca-537.4

(Ejima et al., 2004)

visible | adult stage, with Scer\GAL4^GMR.PU

(Lee et al., 2016)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PU

(Lee et al., 2016)

Detailed Description

Statement

Reference

Expression of sra^Scer\UAS.cEa under the control of Scer\GAL4^GMR.PU results in a mild rough-eye phenotype compared to controls.

(Lee et al., 2016)

Overexpression of sra^Scer\UAS.cEa under the control of Scer\GAL4^elav.PLu in a wild-type background does not affect activity or sleep.

(Nakai et al., 2011)

Expression of sra^Scer\UAS.cEa in developing eyes, driven by Scer\GAL4^GMR.PF, does not cause a phenotype. Expression of sra^Scer\UAS.cEa, under the control of Scer\GAL4^en-e16E, does not cause a phenotype in the wing.

(Takeo et al., 2006)

Expression of sra^Scer\UAS.cEa under the control of Scer\GAL4^elav-C155 or Scer\GAL4^sca-537.4 results in increased ovulation in virgin females compared to control virgin females. Within 30 minutes in a mating assay, only 50% of virgin females expressing sra^Scer\UAS.cEa under the control of Scer\GAL4^elav-C155 have mated, compared to 82% of wild-type virgin females. Virgin females expressing sra^Scer\UAS.cEa under the control of Scer\GAL4^elav-C155 elicit intermediate courtship index (CI) levels (CI is the period of time within the observation period that a male displays any element of courtship behaviour), that are between the level elicited by virgin wild-type females and mated wild-type females. Virgin females expressing sra^Scer\UAS.cEa under the control of Scer\GAL4^elav-C155 have a high extrusion index (EI, the percentage of a time a female shows extrusion behaviour when being courted by a male) which is nearly comparable to that of wild-type mated females. Within 30 minutes in a mating assay, only 65% of virgin females expressing sra^Scer\UAS.cEa under the control of Scer\GAL4^sca-537.4 have mated, compared to 82% of wild-type virgin females. Virgin females expressing sra^Scer\UAS.cEa under the control of Scer\GAL4^sca-537.4 have a significantly higher extrusion index (EI, the percentage of a time a female shows extrusion behaviour when being courted by a male) than wild-type virgin females.

(Ejima et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

sra^UAS.cEa, Scer\GAL4^GMR.PU is an enhancer of visible | adult stage phenotype of Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU

(Lee et al., 2016)

Suppressor of

Statement

Reference

sra^UAS.cEa, Scer\GAL4^{VP16.nanos.UTR} is a suppressor of partially lethal - majority die | germline clone phenotype of Pp2B-14D^DN.UASp, Scer\GAL4^{VP16.nanos.UTR}

(Takeo et al., 2010)

sra^UAS.cEa, Scer\GAL4^how-24B is a suppressor of lethal phenotype of Pp2B-14D^act.UAS, Scer\GAL4^how-24B

(Takeo et al., 2006)

Phenotype Manifest In

Enhancer of

Statement

Reference

sra^UAS.cEa, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU

(Lee et al., 2016)

Suppressor of

Statement

Reference

sra^UAS.cEa, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Pp2B-14D^act.UAS, Scer\GAL4^GMR.PF

(Takeo et al., 2006)

sra^UAS.cEa, Scer\GAL4^GMR.PF is a suppressor of interommatidial bristle phenotype of Pp2B-14D^act.UAS, Scer\GAL4^GMR.PF

(Takeo et al., 2006)

sra^UAS.cEa, Scer\GAL4^en-e16E is a suppressor of wing vein phenotype of Pp2B-14D^act.UAS, Scer\GAL4^en-e16E

(Takeo et al., 2006)

sra^UAS.cEa, Scer\GAL4^en-e16E is a suppressor of wing phenotype of Pp2B-14D^act.UAS, Scer\GAL4^en-e16E

(Takeo et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The severe small and rough eye phenotype observed in flies expressing Hsap\APP^{Aβ42.Scer\UAS.cBa} under the control of Scer\GAL4^GMR.PU is exacerbated further by co-expression of sra^Scer\UAS.cEa.

(Lee et al., 2016)

Co-expression of sra^Scer\UAS.cEa driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16} suppresses the embryonic lethality resulting from the overexpression of Pp2B-14D^{DN.Scer\UAS.P\T} in the female germ-line.

(Takeo et al., 2010)

The rough eye phenotype seen in flies that express Pp2B-14D^act.Scer\UAS under the control of Scer\GAL4^GMR.PF is completely suppressed by coexpression of sra^Scer\UAS.cEa. Additionally, the wing phenotypes seen in flies expressing Pp2B-14D^act.Scer\UAS under the control of Scer\GAL4^en-e16E are completely suppressed by sra^Scer\UAS.cEa coexpression.

(Takeo et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

sra^UAS.cEa/Scer\GAL4^elav.PLu rescues sra^KO

(Nakai et al., 2011)

Comments

Expression of sra^Scer\UAS.cEa in the nervous system under the control of Scer\GAL4^elav.PLu restores the increased locomotor activity found in sra^KO mutants back to wild-type levels. Similarly, sleep is completely restored to wild-type amounts in this genotype. Concurrent with this, a wild-type lifespan is also restored.

(Nakai et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

sra^Scer\UAS.cEa

sra^UAS.cEa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Ejima

(Ejima et al., 2004)

Secondary FlyBase IDs

References (6)

Report Sections

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