FlyBase Allele Report: Dmel\brun[Z3358]

General Information

Symbol

Dmel\brun^Z3358

Species

D. melanogaster

Name

FlyBase ID

FBal0180654

Feature type

allele

Associated gene

Dmel\brun

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Giansanti et al., 2004)

Progenitor genotype

Cytology

Description

Amino acid replacement: ?515term.

(Robinett et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:20,654,125..20,654,125 [-]

Nucleotide change:

C20654125T

Amino acid change:

Q515term | brun-PA

Reported amino acid change:

Q515term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. Reported as a truncation of the polypeptide after residue 514.

(Robinett et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis | male (with Df(2L)pr2b)

(Giansanti et al., 2004)

abnormal cytokinesis | male | recessive

(Giansanti et al., 2004)

abnormal cytokinesis | male | temperature conditional (with Df(2L)pr2b)

(Robinett et al., 2009)

abnormal meiotic cell cycle | male (with Df(2L)pr2b)

(Giansanti et al., 2004)

abnormal meiotic cell cycle | male | recessive

(Giansanti et al., 2004)

abnormal meiotic cell cycle | male | temperature conditional (with Df(2L)pr2b)

(Robinett et al., 2009)

abnormal mitotic cell cycle | male (with Df(2L)pr2b)

(Giansanti et al., 2004)

female fertile (with Df(2L)pr2b)

(Robinett et al., 2009)

male sterile

(Robinett et al., 2009)

male sterile (with Df(2L)Exel7078)

(Riedel et al., 2018)

viable

(Robinett et al., 2009)

viable (with Df(2L)Exel7078)

(Riedel et al., 2018)

Phenotype Manifest In

acroblast (with Df(2L)pr2b)

(Robinett et al., 2009)

contractile ring (with Df(2L)pr2b)

(Robinett et al., 2009)

contractile ring & meiotic telophase I | male

(Giansanti et al., 2004)

contractile ring & meiotic telophase I | male (with Df(2L)pr2b)

(Giansanti et al., 2004)

contractile ring & meiotic telophase II | male

(Giansanti et al., 2004)

contractile ring & meiotic telophase II | male (with Df(2L)pr2b)

(Giansanti et al., 2004)

Nebenkern

(Giansanti et al., 2004)

Nebenkern (with Df(2L)pr2b)

(Giansanti et al., 2004)

spermatid & nucleus | supernumerary

(Giansanti et al., 2004)

spermatid & nucleus | supernumerary (with Df(2L)pr2b)

(Giansanti et al., 2004)

spermatocyte (with Df(2L)pr2b)

(Robinett et al., 2009)

spindle & meiotic telophase I | male

(Giansanti et al., 2004)

spindle & meiotic telophase I | male (with Df(2L)pr2b)

(Giansanti et al., 2004)

spindle & meiotic telophase II | male

(Giansanti et al., 2004)

spindle & meiotic telophase II | male (with Df(2L)pr2b)

(Giansanti et al., 2004)

Detailed Description

Statement

Reference

brun^Z3358/Df(2L)Exel7078 transheterozygous males are near sterile.

(Riedel et al., 2018)

bru^Z3358/Df(2L)pr2b spermatocytes fail to undergo meiotic cytokinesis at 25[o]C. This phenotype is exacerbated at 29[o]C and suppressed at 18[o]C.

Unconstricted contractile rings (visualized with sqh^{RLC.T:Avic\GFP-S65T}) are observed in 65% of bru^Z3358/Df(2L)pr2b telophase spermatocytes, compared to just 10% of wild type telophase spermatocytes (in flies raised at 29[o]C). Unconstricted rings in the mutants eventually fragment.

The quantity, viability and hatching frequency of eggs laid by bru^Z3358/Df(2L)pr2b females are indistinguishable from controls.

bru^Z3358 homozygotes are fully viable through to adulthood at 25[o]C.

Acroblast formation fails in bru^Z3358/Df(2L)pr2b spermatids.

(Robinett et al., 2009)

A large proportion of bru^Z3358 mutant spermatids have one large nebenkern with four nuclei of normal size, indicating a cytokinetic failure in both meiotic divisions. A smaller proportion have two nuclei and a large nebenkern, indicating failure of only one of the meiotic divisions. These defects are also seen in bru^Z3358/Df(2L)pr2b spermatids, except that the proportion of spermatids with two nuclei is higher than those with four. Additionally, bru^Z3358/Df(2L)pr2b spermatids occasionally (<4% of cases) have three nuclei, which may be due to failure of cytokinesis in the first meiotic division, followed by budding off of only one daughter cell in the second meiotic division. A tiny proportion (<1%) have more than four nuclei, which may result from defects in the mitotic divisions preceding mitosis. During early telophase of bru^Z3358 spermatocytes, there is an actin ring of normal appearance and and a normal central spindle. However, as telophase progresses the actin ring fails to fully constrict and the central spindle either becomes less dense or completely disorganized. The cleavage furrow initiates ~10 minutes after anaphase onset as in wild type, but shows a limited ingression and then regresses leading to a failure of cytokinesis. bru^Z3358/Df(2L)pr2b mutants show the same phenotype.

(Giansanti et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

brun^Z3358/Df(2L)pr2b has abnormal meiotic cell cycle | male phenotype, enhanceable by Rab11[+]/Rab11^ETo3

(Robinett et al., 2009)

brun^Z3358/Df(2L)pr2b has abnormal cytokinesis | male phenotype, enhanceable by Rab11[+]/Rab11^ETo3

(Robinett et al., 2009)

Enhancer of

Statement

Reference

bru[+]/brun^Z3358 is an enhancer of abnormal meiotic cell cycle | male phenotype of Rab11^93Bi

(Robinett et al., 2009)

bru[+]/brun^Z3358 is an enhancer of abnormal cytokinesis | male phenotype of Rab11^93Bi

(Robinett et al., 2009)

Other

Statement

Reference

Rab11^93Bi, brun^Z3358 has lethal | larval stage phenotype

(Robinett et al., 2009)

Rab11^93Bi/Rab11^ETo3, brun^Z3358 has lethal | larval stage phenotype

(Robinett et al., 2009)

fwd^Z0453/Df(3L)7C, brun^Z3358/brun^Z0704 has abnormal meiotic cell cycle | male phenotype

(Robinett et al., 2009)

fwd^Z0453/Df(3L)7C, brun^Z3358/brun^Z0704 has abnormal cytokinesis | male phenotype

(Robinett et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

brun^Z3358/Df(2L)pr2b has spermatid phenotype, enhanceable by Rab11[+]/Rab11^ETo3

(Robinett et al., 2009)

Enhancer of

Statement

Reference

bru[+]/brun^Z3358 is an enhancer of spermatid phenotype of Rab11^93Bi

(Robinett et al., 2009)

Other

Statement

Reference

fwd^Z0453/Df(3L)7C, brun^Z3358/brun^Z0704 has spermatid phenotype

(Robinett et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Flies homozygous for both bru^Z3358 and Rab11^93Bi die in early larval stages at 25[o]C, as do flies homozygous for bru^Z3358 and transheterozygous for Rab11^93Bi/Rab11^ETo3.

Testes from bru^Z3358/Df(2L)pr2b;Rab11^ETo3/+ males raised at 25[o]C exhibit a 4.5-fold increase in the percentage of tetranucleate early spermatids relative to testes from bru^Z3358/Df(2L)pr2b males.

bru^Z3358/+ males also homozygous for Rab11^93Bi display a four-fold increase in the total number of multi-nucleate spermatids compared with males homozygous for Rab11^93Bi alone.

Testes from bru^Z0704/bru^Z3358, fwd^Z0453/Df(3L)7C males produce higher percentages of multi-nucleate early spermatids associated with enlarged mitochondrial derivatives than testes from siblings containing one mutant and one wild type copy of either bru or fwd.

The elongation of later-stage spermatids is defective in bru^Z0704/bru^Z3358, fwd^Z0453/Df(3L)7C testes.

The percentage of tetranucleate early spermatids in testes from bru^Z0704/bru^Z3358, pbl⁵/pbl^Z4836 males is similar to that of testes from pbl⁵/pbl^Z4836 males that are heterozygous for a bru mutation, indicating a lack of genetic interaction between bru and pbl.

Mitotic cytokinesis appears normal in bru^Z0704/bru^Z3358, fwd^Z0453/Df(3L)7C third instar larval brains.

(Robinett et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

brun^Z3358/Df(2L)pr2b is rescued by brun^+t5.9

(Robinett et al., 2009)

brun^Z3358/Df(2L)pr2b is rescued by brun^EGFP

(Robinett et al., 2009)

Not rescued by

brun^Z3358/Df(2L)pr2b is not rescued by brun^Ala

(Robinett et al., 2009)

Comments

bru^+t5.9 rescues the male meiotic cytokinesis defects seen in bru^Z3358/Df(2L)pr2b spermatocytes.

bru^{T:Avic\GFP-EGFP} rescues the male meiotic cytokinesis defects seen in bru^Z3358/Df(2L)pr2b spermatocytes.

bru^Ala fails to rescue the male meiotic cytokinesis defects seen in bru^Z3358/Df(2L)pr2b spermatocytes.

(Robinett et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

TRAPPC9^Z3358

(Riedel et al., 2018)

bru^Z3358

(Robinett et al., 2009)

brun^Z3358

Name Synonyms

Secondary FlyBase IDs

References (3)

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