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General Information
Symbol
Dmel\AtpαDTS1
Species
D. melanogaster
Name
FlyBase ID
FBal0188150
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ATPalphaDTS1
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

G20973866A

Amino acid change:

E1021K | Atpalpha-PA; E982K | Atpalpha-PB; E982K | Atpalpha-PC; E982K | Atpalpha-PE; E982K | Atpalpha-PF; E982K | Atpalpha-PG; E982K | Atpalpha-PH; E982K | Atpalpha-PI; E982K | Atpalpha-PJ; E982K | Atpalpha-PK

Reported amino acid change:

E982K

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Atpα protein expression levels are normal in heterozygotes.

Amino acid replacement: E982K.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Heterozygous mutants have a significantly lower respiration (metabolic) rate than controls.

AtpαDTS1/+ flies show a significant reduction in lifespan compared to controls. AtpαDTS1 flies become instantly paralyzed upon exposure to a 15 minute 38oC heat shock. When returned to 22oC, >60% never recover and survivors only begin to show movement after over an hour at the permissive temperature.

AtpαDTS1/+ flies are sluggish compared to wild-type. After exposure to 37-38oC, these mutants become paralyzed within 10-30 seconds with complete penetrance. If this restrictive temperature is maintained for 3 minutes, then flies regain the ability to stand after 1-2 minutes at the permissive temperature and can only walk after another few minutes. Wild-type flies never become paralyzed from exposure to 37-38oC. Although AtpαDTS1/+ flies do not show paralysis in response to mechanical shock when maintained and tested at 20-22oC, bang-sensitive paralysis does occur when flies are tested at 20-22oC if they have been maintained at 28oC. This phenomenon can occur for several hours after placing in the permissive temperature and paralysis lasts around 5-30 seconds. At elevated temperatures (37oC) continuous spiking activity is observed in thoracic readings taken from the dorsal flight muscles of AtpαDTS1 flies, but not in those of wild-type flies. AtpαDTS1 flies have significantly shorter lifespans than wild type and become quite sedentary as they age, with a premature loss of both walking and flight activity. In the brains of middle-aged AtpαDTS1/+ flies, neurodegeneration is evident as the appearance of vacuolar structures throughout the central brain and optic regions. Such structures are rarely seen in wild-type flies. The phenotype is age dependent as young adults (day 2-3 after eclosion) show little neuropathology. This age-dependent neurodegeneration can also be seen in the thoracic ganglion.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

AtpαDTS1 has short lived | dominant phenotype, enhanceable by paraST109/para[+]

AtpαDTS1 has short lived | dominant phenotype, enhanceable by para[+]/parats1

AtpαDTS1 has short lived | dominant phenotype, enhanceable by parats115/para[+]

NOT Enhanced by
Statement
Reference

AtpαDTS1 has short lived | dominant phenotype, non-enhanceable by Df(1)D34/+

AtpαDTS1 has short lived | dominant phenotype, non-enhanceable by Sh14/Sh[+]

AtpαDTS1 has short lived | dominant phenotype, non-enhanceable by eag[+]/eag1

AtpαDTS1 has short lived | dominant phenotype, non-enhanceable by paralk5/para[+]

AtpαDTS1 has short lived | dominant phenotype, non-enhanceable by sei1/sei[+]

NOT Enhancer of
Statement
Reference

AtpαDTS1/AtpαDTS1 is a non-enhancer of short lived phenotype of Sh14, eag1

Phenotype Manifest In
Enhanced by
Statement
Reference

AtpαDTS1 has adult brain phenotype, enhanceable by para[+]/parats1

Enhancer of
Statement
Reference

AtpαDTS1/Atpalpha[+] is an enhancer of adult brain phenotype of parats1

Additional Comments
Genetic Interactions
Statement
Reference

There is no additional reduction in lifespan in eag1, Sh14; AtpαDTS1 triple mutants compared to eag1, Sh14 mutants. The following heterozygous mutants do not enhance the shortened lifespan of AtpαDTS1/+ mutants: eag1, Sh14, and sei1. The short lived phenotype of AtpαDTS1 heterozygotes is enhanced in parats1/+, paraST109/+ and parats115/+ flies. In contrast, heterozygosity for either paralk5 or Df(1)D34 does not shorten the AtpαDTS1 life span. In addition to a shorter lifespan, parats1/+; AtpαDTS1/+ double mutants show an increase in the severity of spongiform neuropathology of the brain at 16 days compared to brains from parats1/+ or AtpαDTS1/+ single mutants. These double mutants show no significant change in temperature sensitivity compared to the single mutants. Like AtpαDTS1 single mutants, parats1; AtpαDTS1 mutants need several minutes to recover from a 3 minute 38oC heat shock. There is no significant change in the lifespan of AtpαDTS1 mutants when they express ShEKO.Scer\UAS.T:Avic\GFP-GL under the control of either Scer\GAL4elav.PLu or Scer\GAL4hs.2sev.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Selected as: a dominant temperature-sensitive paralytic mutation.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (4)