FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\dmt3999
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General Information
Symbol
Dmel\dmt3999
Species
D. melanogaster
Name
FlyBase ID
FBal0191491
Feature type
allele
Associated gene
Dmel\dmt
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Myat et al., 2005)
Progenitor genotype
Cytology
Description

A 10bp deletion that results in a frame shift, introducing 15 novel residues at the C-terminus followed by a stop codon.

(Kerman and Andrew, 2010)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous dmt3999 mutant embryos exhibit frequent breaks and/or gaps in the dorsal trunk, often associated with shorter ventral tracheal branches. Individual salivary ducts often have significant gaps or are completely missing. Defects are also seen in the peripheral nervous system. Inhibiting apoptosis specifically in the trachea (through expression of BacA\p35 under the control of Scer\GAL4btl.PU) significantly rescues the trachea and salivary duct defects.

      dmt3999/dmtI184 mutant embryos exhibit frequent breaks and/or gaps in the dorsal trunk, often associated with shorter ventral tracheal branches. Individual salivary ducts often have significant gaps or are completely missing. Defects are also seen in the peripheral nervous system.

      dmt589/dmt3999 mutant embryos exhibit frequent breaks and/or gaps in the dorsal trunk, often associated with shorter ventral tracheal branches. Individual salivary ducts often have significant gaps or are completely missing.

      dmt3999/Df(3R)GB104 mutant embryos exhibit frequent breaks and/or gaps in the dorsal trunk, often associated with shorter ventral tracheal branches. Individual salivary ducts often have significant gaps or are completely missing. Defects are also seen in the peripheral nervous system.

      (Kerman and Andrew, 2010)

      In aul3999 embryos, the dorsal trunk and ventral branches of the tracheal system appear broken or fail to form at all.

      (Myat et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      dmt589

      (Myat et al., 2005)
      Rescued by

      dmt3999 is rescued by dmtUAS.cKa/Scer\GAL4Tub.PU

      (Kerman and Andrew, 2010)

      dmt3999 is rescued by dmtUAS.N.GFP/Scer\GAL4Tub.PU

      (Kerman and Andrew, 2010)
      Partially rescued by

      dmt3999 is partially rescued by Scer\GAL4btl.PU/dmtUAS.cKa

      (Kerman and Andrew, 2010)

      dmt3999 is partially rescued by dmtUAS.cKa/Scer\GAL4sens.PU

      (Kerman and Andrew, 2010)

      dmt3999 is partially rescued by Scer\GAL4elav.PU/dmtUAS.cKa

      (Kerman and Andrew, 2010)

      dmt3999 is partially rescued by dmtUAS.N.GFP/Scer\GAL4btl.PU

      (Kerman and Andrew, 2010)

      dmt3999 is partially rescued by dmtUAS.N.GFP/Scer\GAL4elav.PU

      (Kerman and Andrew, 2010)

      dmt3999 is partially rescued by dmtUAS.N.GFP/Scer\GAL4sens.PU

      (Kerman and Andrew, 2010)
      Comments

      Global expression of dmtScer\UAS.cKa under the control of Scer\GAL4tub.PU significantly rescues the salivary duct and trachea gap phenotypes seen in homozygous dmt3999 mutant embryos. Trachea and duct-specific expression under the control of Scer\GAL4btl.PU rescues the defects in the duct and trachea. Expression in the peripheral nervous system (PNS) under the control of Scer\GAL4sens.PU or Scer\GAL4elav.PU rescues the PNS and salivary duct defects, but gaps are still observed in the trachea.

      Global expression of dmtScer\UAS.N.T:Avic\GFP under the control of Scer\GAL4tub.PU significantly rescues the salivary duct and trachea gap phenotypes seen in homozygous dmt3999 mutant embryos. Trachea and duct-specific expression under the control of Scer\GAL4btl.PU rescues the defects in the duct and trachea. Expression in the peripheral nervous system (PNS) under the control of Scer\GAL4sens.PU or Scer\GAL4elav.PU rescues the PNS and salivary duct defects, but gaps are still observed in the trachea.

      (Kerman and Andrew, 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Selected as: a mutation that disrupts tracheal development when homozygous.

      (Myat et al., 2005)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      aul3999
      dmt3999
      Name Synonyms
      Secondary FlyBase IDs
        References (2)