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General Information
Symbol
Dmel\cbtEP2237E1
Species
D. melanogaster
Name
FlyBase ID
FBal0191635
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
cbtEP(2)2237E1, cbtE1
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the P{EP} element, resulting in a 3.9kb deletion upstream of the cbt transcription start site.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Wound healing does not appear to be affected compared to controls in cbtEP2237E1/+ wing discs which have been fragmented (a 90[o] sector has been dissected out of the posterior compartment, leaving a 3/4 anterior fragment) and then implanted into wild-type females (analysed at 24 and 48 hours after implantation). However, the cbtEP2237E1/+ regenerating discs show significantly impaired proliferation compared to controls.

cbtEP2237E1 mutants show a dorsal closure phenotype. During closure, the dorsal epidermal cells of cbtEP2237E1 embryos do not elongate to the same extent as wild type and sometimes show defective planar polarization. Cytoskeleton activity at the leading edge is disrupted; cbtEP2237E1 mutants do not display the characteristic aggregates of muscle myosin and filamentous actin seen in wild type. Morphogenesis of the amnioserosa is not affected in cbtEP2237E1 embryos, but some mutants show amnioserosa-epidermis attachment defects. By the end of embryonic development, over 98% of cbtEP2237E1 embryos have dorsal holes that cover most of the dorsal region of the cuticle and some of these embryos have extruded internal organs.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The presence of a cbtEP2237E1 background mutation reduces embryonic lethality from 100 to 36.4% when hepAct.Scer\UAS is expressed under the control of Scer\GAL464B.

The presence of a cbtEP2237E1 background mutation reduces embryonic lethality from 100 to 36.4% when hepAct.Scer\UAS is expressed under the control of Scer\GAL469B.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of cbtScer\UAS.cMa, under the control of Scer\GAL4da.G32, results in a 50.2% rescue of lethality in cbtEP2237E1 mutants. Expression of cbtScer\UAS.cMa in the embryonic epidermis, driven by Scer\GAL469B, completely rescues lethality, while expression in the yolk cell, driven by Scer\GAL4P0180, results in a 60% rescue of lethality.

Expression of cbtScer\UAS.cMa, under the control of Scer\GAL4da.G32, results in a 50.2% rescue of lethality in cbtEP2237E1 mutants. Expression of cbtScer\UAS.cMa in the embryonic epidermis, driven by Scer\GAL464B, completely rescues lethality, while expression in the yolk cell, driven by Scer\GAL4P0180, results in a 60% rescue of lethality.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (7)