FlyBase Allele Report: Dmel\cbt[EP2237E1]

General Information

Symbol

Dmel\cbt^EP2237E1

Species

D. melanogaster

Name

FlyBase ID

FBal0191635

Feature type

allele

Associated gene

Dmel\cbt

Associated Insertion(s)

Carried in Construct

Also Known As

cbt^EP(2)2237E1, cbt^E1

Key Links

Allele class

loss of function allele

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

loss of function allele

(Munoz-Descalzo et al., 2005)

Mutagen

Delta2-3 transposase

(Munoz-Descalzo et al., 2005)

Progenitor genotype

cbt^EP2237

(Munoz-Descalzo et al., 2005)

Cytology

Description

Imprecise excision of the P{EP} element, resulting in a 3.9kb deletion upstream of the cbt transcription start site.

(Munoz-Descalzo et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity | embryonic stage

(Munoz-Descalzo et al., 2005)

decreased occurrence of cell division | larval stage | conditional

(Blanco et al., 2010)

lethal - all die during embryonic stage

(Belacortu et al., 2011, Munoz-Descalzo et al., 2005)

some die during embryonic stage

(Belacortu et al., 2011, Munoz-Descalzo et al., 2005)

Phenotype Manifest In

amnioserosa

(Munoz-Descalzo et al., 2005)

embryo | dorsal closure stage

(Munoz-Descalzo et al., 2005)

embryonic/first instar larval cuticle | dorsal

(Munoz-Descalzo et al., 2005)

embryonic leading edge cell

(Munoz-Descalzo et al., 2005)

leading edge & filamentous actin

(Munoz-Descalzo et al., 2005)

leading edge & muscle myosin complex

(Munoz-Descalzo et al., 2005)

Detailed Description

Statement

Reference

Wound healing does not appear to be affected compared to controls in cbt^EP2237E1/+ wing discs which have been fragmented (a 90[o] sector has been dissected out of the posterior compartment, leaving a 3/4 anterior fragment) and then implanted into wild-type females (analysed at 24 and 48 hours after implantation). However, the cbt^EP2237E1/+ regenerating discs show significantly impaired proliferation compared to controls.

(Blanco et al., 2010)

cbt^EP2237E1 mutants show a dorsal closure phenotype. During closure, the dorsal epidermal cells of cbt^EP2237E1 embryos do not elongate to the same extent as wild type and sometimes show defective planar polarization. Cytoskeleton activity at the leading edge is disrupted; cbt^EP2237E1 mutants do not display the characteristic aggregates of muscle myosin and filamentous actin seen in wild type. Morphogenesis of the amnioserosa is not affected in cbt^EP2237E1 embryos, but some mutants show amnioserosa-epidermis attachment defects. By the end of embryonic development, over 98% of cbt^EP2237E1 embryos have dorsal holes that cover most of the dorsal region of the cuticle and some of these embryos have extruded internal organs.

(Munoz-Descalzo et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

cbt^EP2237E1 is a suppressor of increased cell number | adult stage phenotype of mir-958^KO

(Mukherjee et al., 2021)

cbt^EP2237E1/Scer\GAL4^69B is a suppressor of lethal | embryonic stage phenotype of hep^Act.UAS

(Munoz-Descalzo et al., 2005)

Phenotype Manifest In

Suppressor of

Statement

Reference

cbt^EP2237E1 is a suppressor of intestinal stem cell | increased number | adult stage phenotype of mir-958^KO

(Mukherjee et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

The presence of a cbt^EP2237E1 background mutation reduces embryonic lethality from 100 to 36.4% when hep^Act.Scer\UAS is expressed under the control of Scer\GAL4^69B.

The presence of a cbt^EP2237E1 background mutation reduces embryonic lethality from 100 to 36.4% when hep^Act.Scer\UAS is expressed under the control of Scer\GAL4^64B.

(Munoz-Descalzo et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

cbt^EP2237E1 is rescued by cbt^UAS.cMa/Scer\GAL4^69B

(Munoz-Descalzo et al., 2005)

Partially rescued by

cbt^EP2237E1 is partially rescued by Scer\GAL4^arm.PS/cbt^UAS.cMa

(Belacortu et al., 2011)

cbt^EP2237E1 is partially rescued by Scer\GAL4^P0180/cbt^UAS.cMa

(Munoz-Descalzo et al., 2005)

cbt^EP2237E1 is partially rescued by Scer\GAL4^da.PU/cbt^UAS.cMa

(Munoz-Descalzo et al., 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

cbt^E1

(Mukherjee et al., 2021, Bartok et al., 2015, Bejarano et al., 2008)

cbt^EP(2)2237E1

(Belacortu et al., 2011, Blanco et al., 2010, Munoz-Descalzo et al., 2005)

cbt^EP2237E1

(Mukherjee et al., 2021, Rodriguez, 2011)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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