FlyBase Allele Report: Dmel\S6kII[D1]

General Information

Symbol

Dmel\S6kII^D1

Species

D. melanogaster

Name

FlyBase ID

FBal0194032

Feature type

allele

Associated gene

Dmel\S6kII

Associated Insertion(s)

Carried in Construct

Also Known As

RSK^D1

Key Links

Allele class

loss of function allele

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

loss of function allele

(Kim et al., 2006)

Mutagen

Delta2-3 transposase

(Kim et al., 2006)

Progenitor genotype

S6kII^EP7363

(Kim et al., 2006)

Cytology

Description

Excision of the P{EP} element, resulting in a 3.1kb deletion that removes the first exon of the S6kII gene (nucleotides 95440-98535 of AE003574) as well as some parts of the putative promoter region and the first intron.

(Kim et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Beck et al., 2015)

semi-fertile

(Kim et al., 2006)

short lived

(Kim et al., 2006)

viable

(Kim et al., 2006)

visible (with Df(1)R8A)

(Kim et al., 2006)

visible | recessive

(Kim et al., 2006)

Phenotype Manifest In

cone cell

(Kim et al., 2006)

cone cell (with Df(1)R8A)

(Kim et al., 2006)

embryonic/larval neuromuscular junction

(Beck et al., 2015)

eye photoreceptor cell

(Kim et al., 2006)

eye photoreceptor cell (with Df(1)R8A)

(Kim et al., 2006)

NMJ bouton

(Beck et al., 2015)

NMJ bouton | increased number

(Beck et al., 2015)

ommatidial precursor cluster

(Kim et al., 2006)

ommatidial precursor cluster (with Df(1)R8A)

(Kim et al., 2006)

pigment cell

(Kim et al., 2006)

pigment cell (with Df(1)R8A)

(Kim et al., 2006)

presynaptic active zone

(Beck et al., 2015)

wing vein | ectopic

(Kim et al., 2006)

wing vein | ectopic (with Df(1)R8A)

(Kim et al., 2006)

Detailed Description

Statement

Reference

NMJ size is significantly reduced in S6kII^D1 mutants compared with wild type controls. The number and area of pre-synaptic active zones per NMJ and number and area of post-synaptic GluRIID fields are also reduced. The ratio between active zones and GluRIID fields is unchanged from wild type and no apposition defects are observed. Bouton number is increased, but they are smaller in size and contain fewer synaptic sites.

(Beck et al., 2015)

S6kII^D1 flies are short lived and show a disarrayed eye structure with increased numbers of photoreceptor rhabdomeres, cone cells and pigment cells, which become more pronounced at higher temperatures, as compared to controls; these mutants show ectopic differentiation of retinal cells at both the pupal and the adult stage. S6kII^D1 heterozygotes and hemizygotes, however, show normal eyes. Around 16% of S6kII^D1 flies raised at 25[o]C show ectopic veins in various regions of the wing, which becomes more severe at 29[o]C, as compared to controls. S6kII^D1/Df(1)R8A transheterozygotes display identical eye and wing phenotypes to S6kII^D1 homozygotes.

(Kim et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

S6kII^D1/S6kII[+] is an enhancer of visible phenotype of Ras85D^V12.UAS, Scer\GAL4^hs.2sev

(Kim et al., 2006)

S6kII^D1/S6kII[+] is an enhancer of visible phenotype of Raf^UAS.F179, Scer\GAL4^hs.2sev

(Kim et al., 2006)

Suppressor of

Statement

Reference

S6kII^D1/S6kII[+] is a suppressor of lethal | male | heat sensitive phenotype of Raf¹²

(Kim et al., 2006)

S6kII^D1/S6kII[+] is a suppressor of visible phenotype of Raf¹²

(Kim et al., 2006)

Phenotype Manifest In

Enhancer of

Statement

Reference

S6kII^D1/S6kII[+] is an enhancer of ommatidium phenotype of Ras85D^V12.UAS, Scer\GAL4^hs.2sev

(Kim et al., 2006)

S6kII^D1/S6kII[+] is an enhancer of interommatidial bristle phenotype of Ras85D^V12.UAS, Scer\GAL4^hs.2sev

(Kim et al., 2006)

S6kII^D1/S6kII[+] is an enhancer of rhabdomere phenotype of Ras85D^V12.UAS, Scer\GAL4^hs.2sev

(Kim et al., 2006)

S6kII^D1/S6kII[+] is an enhancer of ommatidium phenotype of Raf^UAS.F179, Scer\GAL4^hs.2sev

(Kim et al., 2006)

S6kII^D1/S6kII[+] is an enhancer of interommatidial bristle phenotype of Raf^UAS.F179, Scer\GAL4^hs.2sev

(Kim et al., 2006)

S6kII^D1/S6kII[+] is an enhancer of rhabdomere phenotype of Raf^UAS.F179, Scer\GAL4^hs.2sev

(Kim et al., 2006)

Suppressor of

Statement

Reference

S6kII^D1/S6kII[+] is a suppressor of rhabdomere phenotype of Raf¹²

(Kim et al., 2006)

S6kII^D1/S6kII[+] is a suppressor of ommatidium phenotype of Raf¹²

(Kim et al., 2006)

S6kII^D1/S6kII[+] is a suppressor of interommatidial bristle phenotype of Raf¹²

(Kim et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The roughened eye and increased rhabdomere phenotypes seen when either phl^{Scer\UAS.F179} or Ras85D^V12.Scer\UAS is expressed under the control of Scer\GAL4^hs.2sev are enhanced in a S6kII^D1/+ or S6kII^D1/Y background.

(Kim et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

S6kII^D1 is rescued by Scer\GAL4^da.G32/S6kII^UAS.Tag:MYC

(Kim et al., 2006)

S6kII^D1 is rescued by S6kII^{K231R.UAS.Tag:MYC}/Scer\GAL4^da.G32

(Kim et al., 2006)

S6kII^D1 is rescued by S6kII^{K231R.K597R.UAS.Tag:MYC}/Scer\GAL4^da.G32

(Kim et al., 2006)