FlyBase Allele Report: Dmel\DAAM[UASp.cMa]

General Information

Symbol

Dmel\DAAM^UASp.cMa

Species

D. melanogaster

Name

FlyBase ID

FBal0194064

Feature type

allele

Associated gene

Dmel\DAAM

Associated Insertion(s)

Carried in Construct

P{UASp-DAAM.FL.M}

Also Known As

UAS-FLDAAM

Key Links

Transgenic product class

cDNA

(Matusek et al., 2006)

wild_type

(Matusek et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Matusek et al., 2006)

wild_type

(Matusek et al., 2006)

Mutagen

in vitro construct

(Matusek et al., 2006)

Progenitor genotype

Carried in construct

P{UASp-DAAM.FL.M}

Cytology

Description

UASp regulatory sequences drive expression of full-length DAAM (sequence corresponds to RE67944).

(Matusek et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

DAAM

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | late third instar larval stage, with Scer\GAL4^Mef2.PU

(Molnár et al., 2014)

Phenotype Manifest In

filamentous actin & tracheal fusion cell, with DAAM^C.UASp, Scer\GAL4^btl.PS

(Matusek et al., 2006)

larval somatic muscle cell | late third instar larval stage, with Scer\GAL4^Mef2.PU

(Molnár et al., 2014)

muscle cell of A1-7 ventral longitudinal muscle 3 | late third instar larval stage, with Scer\GAL4^Mef2.PU

(Molnár et al., 2014)

sarcomere | late third instar larval stage, with Scer\GAL4^Mef2.PU

(Molnár et al., 2014)

Detailed Description

Statement

Reference

Neuronal expression of DAAM^{Scer\UAS.P\T.cMa} under the control of Scer\GAL4^elav.PU has no effect on viability and only affects the axonal projection pattern of the mushroom body in 2-3% of cases.

(Gombos et al., 2015)

Expression of DAAM^{Scer\UAS.P\T.cMa} under the control of Scer\GAL4^Mef2.PU results in late third instar larvae that are significantly longer than controls. The LV3 muscle is longer than normal, although sarcomere size is slightly shorter than controls. The increase in muscle length is due to a significant increase in sarcomere number. The larvae show increased motility compared to wild type.

Expression of DAAM^{Scer\UAS.P\T.cMa} under the control of Scer\GAL4^UH3 has no obvious effect on the indirect flight muscles.

(Molnár et al., 2014)

Coexpression of DAAM^{Scer\UAS.P\T.cMa} and DAAM^{C.Scer\UAS.P\T}, under the control of Scer\GAL4^btl.PS, causes a suppression of the Scer\GAL4^btl.PS/DAAM^{C.Scer\UAS.P\T} phenotype in regular tracheal cells but not in fusion cells.

(Matusek et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

DAAM^UASp.cMa, Scer\GAL4^ptc.PU is a non-enhancer of visible | adult stage phenotype of Dora^GD6527, Scer\GAL4^ptc.PU

(Molina-Pelayo et al., 2022)

NOT Suppressor of

Statement

Reference

DAAM^UASp.cMa, Scer\GAL4^ptc.PU is a non-suppressor of visible | adult stage phenotype of Dora^GD6527, Scer\GAL4^ptc.PU

(Molina-Pelayo et al., 2022)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

DAAM^UASp.cMa, Scer\GAL4^ptc.PU is a non-enhancer of wing hair phenotype of Dora^GD6527, Scer\GAL4^ptc.PU

(Molina-Pelayo et al., 2022)

NOT Suppressor of

Statement

Reference

DAAM^UASp.cMa, Scer\GAL4^ptc.PU is a non-suppressor of wing hair phenotype of Dora^GD6527, Scer\GAL4^ptc.PU

(Molina-Pelayo et al., 2022)

DAAM^UASp.cMa, Scer\GAL4^Hk-UH3 is a non-suppressor of striated muscle thin filament phenotype of Scer\GAL4^Hk-UH3, tmod^UAS.cBa

(Molnár et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The thin filament shortening phenotype seen in the indirect flight muscles of animals expressing tmod^Scer\UAS.cBa under the control of Scer\GAL4^UH3 is unaffected by the co-expression of DAAM^{Scer\UAS.P\T.cMa}.

(Molnár et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

DAAM^UASp.cMa/Scer\GAL4^elav.PLu rescues DAAM^Ex4

(Földi et al., 2022)

DAAM^UASp.cMa/Scer\GAL4^ey-OK107 rescues DAAM^Ex4

(Földi et al., 2022)

DAAM^UASp.cMa/Scer\GAL4^elav.PU rescues DAAM^Ex1

(Gombos et al., 2015)

DAAM^UASp.cMa/Scer\GAL4^elav.PU rescues DAAM^Ex4

(Gombos et al., 2015)

DAAM^UASp.cMa/Scer\GAL4^Hk-UH3 rescues DAAM^Ex1

(Molnár et al., 2014)

Scer\GAL4^elav-C155/DAAM^UASp.cMa rescues DAAM^Ex1/DAAM^Ex68

(Matusek et al., 2008)

DAAM^UASp.cMa/Scer\GAL4^Act5C.PI rescues DAAM^Ex1/DAAM^Ex68

(Matusek et al., 2008)

DAAM^UASp.cMa/Scer\GAL4^Act.PU rescues DAAM^Ex249

(Matusek et al., 2006)

DAAM^UASp.cMa/Scer\GAL4^Tub.PU rescues DAAM^Ex249

(Matusek et al., 2006)

DAAM^UASp.cMa/Scer\GAL4^Act.PU rescues DAAM^Ex184

(Matusek et al., 2006)

DAAM^UASp.cMa/Scer\GAL4^Tub.PU rescues DAAM^Ex184

(Matusek et al., 2006)

DAAM^UASp.cMa/Scer\GAL4^Act.PU rescues DAAM^Ex1

(Matusek et al., 2006)

DAAM^UASp.cMa/Scer\GAL4^Tub.PU rescues DAAM^Ex1

(Matusek et al., 2006)

DAAM^UASp.cMa/Scer\GAL4^Act.PU rescues DAAM^Ex65

(Matusek et al., 2006)

DAAM^UASp.cMa/Scer\GAL4^Tub.PU rescues DAAM^Ex65

(Matusek et al., 2006)

DAAM^UASp.cMa/Scer\GAL4^Act.PU rescues DAAM^Ex202

(Matusek et al., 2006)

DAAM^UASp.cMa/Scer\GAL4^Tub.PU rescues DAAM^Ex202

(Matusek et al., 2006)

DAAM^UASp.cMa/Scer\GAL4^Act.PU rescues DAAM^Ex36

(Matusek et al., 2006)

DAAM^UASp.cMa/Scer\GAL4^Tub.PU rescues DAAM^Ex36

(Matusek et al., 2006)

DAAM^UASp.cMa/Scer\GAL4^Act.PU rescues DAAM^Ex68

(Matusek et al., 2006)

DAAM^UASp.cMa/Scer\GAL4^Tub.PU rescues DAAM^Ex68

(Matusek et al., 2006)

Partially rescues

DAAM^UASp.cMa/Scer\GAL4^Mef2.PU partially rescues DAAM^Ex68

(Molnár et al., 2014)

DAAM^UASp.cMa/Scer\GAL4^btl.PS partially rescues DAAM^Ex68

(Matusek et al., 2006)

Comments

Expression of DAAM^{Scer\UAS.P\T.cMa} under the control of Scer\GAL4^elav.PU rescues the α and β lobe mushroom body defects seen in DAAM^Ex1 mutant flies.

Expression of DAAM^{Scer\UAS.P\T.cMa} under the control of Scer\GAL4^elav.PU rescues the α and β lobe mushroom body defects seen in DAAM^Ex4 mutant flies.

(Gombos et al., 2015)

Expression of DAAM^{Scer\UAS.P\T.cMa} under the control of Scer\GAL4^UH3 rescues the flightless phenotype seen in some DAAM^Ex1 adults.

Expression of DAAM^{Scer\UAS.P\T.cMa} under the control of Scer\GAL4^Mef2.PU partially rescues the reduced motility and almost fully rescues the body and muscle size of late third instar DAAM^Ex68 larvae.

(Molnár et al., 2014)

The CNS defects revealed in DAAM^Ex68/DAAM^Ex1 mutants are fully rescued when DAAM^{Scer\UAS.P\T.cMa} is expressed under the control of the pan-neuronal Scer\GAL4^elav-C155 or the ubiquitous Scer\GAL4^Act5C.PI driver.

(Matusek et al., 2008)

Expression of DAAM^{Scer\UAS.P\T.cMa} under the control of Scer\GAL4^btl.PS rescues the tracheal phenotype of DAAM^Ex68 mutants.

(Matusek et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

DAAM^{Scer\UAS.P\T.cMa}

DAAM^UASp.cMa

Name Synonyms

Secondary FlyBase IDs

References (7)

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