FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\scribΔPDZs.UASp.GFP
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General Information
Symbol
Dmel\scribΔPDZs.UASp.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0196601
Feature type
allele
Associated gene
Dmel\scrib
Associated Insertion(s)
Carried in Construct
P{UASp-scrib.ΔPDZs.GFP}
Key Links
Transgenic product class
inframe_deletion
(Zeitler et al., 2004)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Zeitler et al., 2004)
Mutagen
in vitro construct
(Zeitler et al., 2004)
Progenitor genotype
Carried in construct
P{UASp-scrib.ΔPDZs.GFP}
Cytology
Description

UASp regulatory sequences drive expression of amino acids 1-692 of the scrib product (which therefore lacks the four PDZ domains) containing a COOH-terminal GFP tag.

(Zeitler et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
scrib
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      scribΔPDZs.UASp.GFP/Scer\GAL469B partially rescues scribird15/scrib673

      (Zeitler et al., 2004)

      scribΔPDZs.UASp.GFP/Scer\GAL469B partially rescues scribdt6/scrib673

      (Zeitler et al., 2004)

      scribΔPDZs.UASp.GFP/Scer\GAL469B partially rescues scribj7B3/scrib673

      (Zeitler et al., 2004)
      Fails to rescue

      scribΔPDZs.UASp.GFP, Scer\GAL469B, scribΔLRR.UASp.Tag:Myr(Src42A),GFP fails to rescue scribird15/scrib673

      (Zeitler et al., 2004)
      Comments

      Expression of scribΔPDZs.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL469B fully rescues the cell polarization phenotype but only partially rescues the disc overgrowth phenotype of scrib673/scribird15 transheterozygotes.

      Co-expression of scribΔLRR.Scer\UAS.P\T.T:Myr6,T:Avic\GFP and scrib<up>ΔPDZs.Scer\UAS.P\T. T:Avic \GFP</up> under the control of Scer\GAL469B fails to rescue the cell polarization and disc overgrowth phenotypes of scrib673/scribird15 transheterozygotes, even when expression levels are increased via raising larvae at 29 oC.

      Expression of scribΔPDZs.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL469B provides a more complete rescue of the size phenotype of scrib673/scribdt6 and scrib673/scribj7B3 transheterozygous discs compared to scrib null discs. Increasing the level of transgene expression via raising larvae at 29 oC further increases this rescue. Moreover, the apicolateral enhancement of septate junction markers is restored and cell shape is columnar rather than cuboidal in rescued scrib673/scribdt6 discs.

      (Zeitler et al., 2004)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      scribΔPDZs.Scer\UAS.P\T.T:Avic\GFP
      scribΔPDZs.UASp.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (2)