FlyBase Allele Report: Dmel\scrib[dt6]

General Information

Symbol

Dmel\scrib^dt6

Species

D. melanogaster

Name

FlyBase ID

FBal0103573

Feature type

allele

Associated gene

Dmel\scrib

Associated Insertion(s)

Carried in Construct

Also Known As

dt6, scrib⁴

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Zeitler et al., 2004)

Mutagen

ethyl methanesulfonate

(Zeitler et al., 2004, Kidd et al., 1999)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | larval stage (with scrib⁶⁷³)

(Zeitler et al., 2004)

abnormal size | larval stage (with scrib⁶⁷³)

(Zeitler et al., 2004)

increased cell number | larval stage (with scrib⁶⁷³)

(Zeitler et al., 2004)

lethal | recessive

(Kidd et al., 1999)

lethal - all die before end of pupal stage (with scrib¹)

(Zeitler et al., 2004)

lethal - all die before end of pupal stage (with scrib⁶⁷³)

(Zeitler et al., 2004)

lethal - all die before end of pupal stage (with scrib⁸⁸²)

(Zeitler et al., 2004)

lethal - all die before end of pupal stage (with scrib^dt14)

(Zeitler et al., 2004)

lethal - all die before end of pupal stage (with scrib^ird15)

(Zeitler et al., 2004)

some die during pupal stage (with scrib¹)

(Zeitler et al., 2004)

some die during pupal stage (with scrib⁶⁷³)

(Zeitler et al., 2004)

some die during pupal stage (with scrib⁸⁸²)

(Zeitler et al., 2004)

some die during pupal stage (with scrib^dt14)

(Zeitler et al., 2004)

some die during pupal stage (with scrib^ird15)

(Zeitler et al., 2004)

visible | recessive | somatic clone

(Zeitler et al., 2004)

Phenotype Manifest In

embryonic dorsal epidermis | germline clone

(Zeitler et al., 2004)

epithelial cell (with scrib⁶⁷³)

(Zeitler et al., 2004)

eye | somatic clone

(Zeitler et al., 2004)

septate junction (with scrib⁶⁷³)

(Zeitler et al., 2004)

wing disc (with scrib⁶⁷³)

(Zeitler et al., 2004)

Detailed Description

Statement

Reference

Like wild type, cells from scrib^dt6/scrib⁶⁷³ transheterozygous wing discs are arranged in epithelial monolayers and maintain the folded structure of the tissue. However, these mutant scrib cells show cuboidal rather than columnar morphology.

scrib^dt6/scrib⁶⁷³ transheterozygous wing discs show an intermediate increase in both the surface area and volume compared to wild type and contain approximately 300% more cells than wild type.

Adult eye tissue homozygous for scrib^dt6 is readily obtained, although it is rough, enlarged and folded.

scrib^dt6 germ line clone embryos display a consistent failure of dorsal closure and mildly penetrant anterior open defects.

Adherens junction formation is normal but septate junctions are severely disrupted in scrib^dt6/scrib⁶⁷³ transheterozygous wing discs.

(Zeitler et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

scrib⁸⁸²

(Zeitler et al., 2004)

scrib^ird15

(Zeitler et al., 2004)

scrib¹

(Zeitler et al., 2004)

scrib⁶⁷³

(Zeitler et al., 2004)

scrib^dt14

(Zeitler et al., 2004)

scrib⁶⁷³

(Kidd et al., 1999)

scrib^dt12

(Kidd et al., 1999)

scrib^dt14

(Kidd et al., 1999)

Partially rescued by

scrib^dt6/scrib⁶⁷³ is partially rescued by scrib^{ΔPDZs.UASp.GFP}/Scer\GAL4^69B

(Zeitler et al., 2004)

scrib^dt6/scrib⁶⁷³ is partially rescued by scrib^4.UASp.GFP/Scer\GAL4^69B

(Zeitler et al., 2004)

Not rescued by

scrib^dt6/scrib⁶⁷³ is not rescued by scrib^{ΔLRR.UASp.GFP}/Scer\GAL4^69B

(Zeitler et al., 2004)

scrib^dt6/scrib⁶⁷³ is not rescued by Scer\GAL4^69B/scrib^{ΔLRR.UASp.Tag:Myr(Src42A),GFP}

(Zeitler et al., 2004)

Comments

Expression of scrib^{ΔLRR.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^69B fails to rescue the cell polarization and disc overgrowth phenotypes of scrib⁶⁷³/scrib^dt6 transheterozygotes.

Expression of scrib^{ΔLRR.Scer\UAS.P\T.T:Myr6,T:Avic\GFP} under the control of Scer\GAL4^69B fails to rescue the cell polarization and disc overgrowth phenotypes of scrib⁶⁷³/scrib^dt6 transheterozygotes.

Expression of scrib^{4.Scer\UAS.P\T.T:Avic\GFP} or scrib^{ΔPDZs.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^69B provides a more complete rescue of the size phenotype of scrib⁶⁷³/scrib^dt6 transheterozygous discs compared to scrib null discs. Increasing the level of transgene expression via raising larvae at 29 ^oC further increases this rescue. Moreover, the apicolateral enhancement of septate junction markers is restored and cell shape is columnar rather than cuboidal in rescued scrib⁶⁷³/scrib^dt6 discs.

(Zeitler et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

29013

scrib^dt6 red¹ e¹/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

The reason for the early lethality of scrib^dt6/scrib^dt12 and scrib^dt6/Df(3R)Tl-X transheterozygotes is not known but may be because scrib^dt6 and scrib^dt12 were generated on a similar parental chromosome and share an accessory lethal.

(Zeitler et al., 2004)

Comments

Heteroallelic combinations between scrib^dt6, scrib^dt12 and scrib⁸⁸² suggest that scrib^dt6 is the most severe mutation of these three.

(Zeitler et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

dt6

(Zeitler et al., 2004, Kidd et al., 1999)

scrib 4

(Zeitler et al., 2004)

scrib⁴

(Khoury and Bilder, 2020, Bahri et al., 2010)

scrib^dt6

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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