FlyBase Allele Report: Dmel\scrib[ird15]

General Information

Symbol

Dmel\scrib^ird15

Species

D. melanogaster

Name

FlyBase ID

FBal0127890

Feature type

allele

Associated gene

Dmel\scrib

Associated Insertion(s)

Carried in Construct

Also Known As

ird15

Key Links

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Zeitler et al., 2004)

Mutagen

ethyl methanesulfonate

(Zeitler et al., 2004, Wu et al., 2001)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity | larval stage (with scrib⁶⁷³)

(Zeitler et al., 2004)

abnormal cell shape | larval stage (with scrib⁶⁷³)

(Zeitler et al., 2004)

abnormal developmental rate | third instar larval stage (with Df(3R)Tl-X)

(Zeitler et al., 2004)

abnormal developmental rate | third instar larval stage (with scrib⁶⁷³)

(Zeitler et al., 2004)

abnormal immune response | recessive

(Wu et al., 2001)

abnormal size | larval stage (with scrib⁶⁷³)

(Zeitler et al., 2004)

increased body size | third instar larval stage (with Df(3R)Tl-X)

(Zeitler et al., 2004)

increased body size | third instar larval stage (with scrib¹)

(Zeitler et al., 2004)

increased body size | third instar larval stage (with scrib⁶⁷³)

(Zeitler et al., 2004)

increased cell number | larval stage (with scrib⁶⁷³)

(Zeitler et al., 2004)

lethal | recessive

(Wu et al., 2001)

lethal - all die before end of larval stage (with Df(3R)Tl-X)

(Zeitler et al., 2004)

lethal - all die before end of larval stage (with scrib¹)

(Zeitler et al., 2004)

lethal - all die before end of larval stage (with scrib⁶⁷³)

(Zeitler et al., 2004)

lethal - all die before end of P-stage | somatic clone

(Zeitler et al., 2004)

lethal - all die before end of pupal stage (with scrib⁸⁸²)

(Zeitler et al., 2004)

lethal - all die before end of pupal stage (with scrib^dt6)

(Zeitler et al., 2004)

lethal - all die before end of pupal stage (with scrib^dt12)

(Zeitler et al., 2004)

lethal - all die before end of pupal stage (with scrib^dt14)

(Zeitler et al., 2004)

some die during pharate adult stage | somatic clone

(Zeitler et al., 2004)

some die during pupal stage (with scrib⁸⁸²)

(Zeitler et al., 2004)

some die during pupal stage (with scrib^dt6)

(Zeitler et al., 2004)

some die during pupal stage (with scrib^dt12)

(Zeitler et al., 2004)

some die during pupal stage (with scrib^dt14)

(Zeitler et al., 2004)

some die during third instar larval stage (with Df(3R)Tl-X)

(Zeitler et al., 2004)

some die during third instar larval stage (with scrib¹)

(Zeitler et al., 2004)

some die during third instar larval stage (with scrib⁶⁷³)

(Zeitler et al., 2004)

Phenotype Manifest In

adult head | somatic clone

(Zeitler et al., 2004)

epithelial cell (with scrib⁶⁷³)

(Zeitler et al., 2004)

wing disc (with scrib⁶⁷³)

(Zeitler et al., 2004)

Detailed Description

Statement

Reference

Cells from scrib^ird15/scrib⁶⁷³ transheterozygous wing discs are round (rather than elongated as in wild type) and have a multilayered organisation (rather than monolayered as in wild type), forming a solid spherical mass rather than the flat disc seen in wild type animals.

scrib^ird15/scrib⁶⁷³ transheterozygous wing discs show a dramatic increase in both the surface area and volume compared to wild type and contain approximately 500% more cells than wild type.

Animals with eyes homozygous for scrib^ird15 never eclose, instead dying as headless pharate adults.

(Zeitler et al., 2004)

Mutant larvae do not show normal melanisation at a wound site in response to infection. They do not have melanotic tumours.

(Wu et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

scrib⁸⁸²

(Zeitler et al., 2004)

scrib¹

(Zeitler et al., 2004)

scrib⁶⁷³

(Zeitler et al., 2004)

scrib^dt12

(Zeitler et al., 2004)

scrib^dt14

(Zeitler et al., 2004)

scrib^dt6

(Zeitler et al., 2004)

scrib⁸⁸²

(Wu et al., 2001)

scrib⁶⁷³

(Wu et al., 2001)

scrib^j7B3

(Wu et al., 2001)

Rescued by

scrib^ird15/scrib⁶⁷³ is rescued by Scer\GAL4^69B/scrib^UASp.GFP

(Zeitler et al., 2004)

scrib^ird15/scrib⁶⁷³ is rescued by scrib^{ΔPDZ3.4.UASp.GFP}/Scer\GAL4^69B

(Zeitler et al., 2004)

scrib^ird15/scrib⁶⁷³ is rescued by scrib^UAS.cBa/Scer\GAL4^69B

(Zeitler et al., 2004)

Partially rescued by

scrib^ird15/scrib⁶⁷³ is partially rescued by scrib^{ΔPDZs.UASp.GFP}/Scer\GAL4^69B

(Zeitler et al., 2004)

scrib^ird15/scrib⁶⁷³ is partially rescued by scrib^4.UASp.GFP/Scer\GAL4^69B

(Zeitler et al., 2004)

Not rescued by

scrib^ird15/scrib⁶⁷³ is not rescued by scrib^{ΔLRR.UASp.GFP}/Scer\GAL4^69B

(Zeitler et al., 2004)

scrib^ird15/scrib⁶⁷³ is not rescued by scrib^{ΔPDZs.UASp.GFP}/Scer\GAL4^69B/scrib^{ΔLRR.UASp.Tag:Myr(Src42A),GFP}

(Zeitler et al., 2004)

scrib^ird15/scrib⁶⁷³ is not rescued by Scer\GAL4^69B/scrib^{ΔLRR.UASp.Tag:Myr(Src42A),GFP}

(Zeitler et al., 2004)

scrib^ird15/scrib⁶⁷³ is not rescued by scrib^{L223Q.UASp.GFP}/Scer\GAL4^69B

(Zeitler et al., 2004)

Comments

Expression of scrib^Scer\UAS.cBa under the control of Scer\GAL4^69B fully rescues the cell polarization and disc overgrowth phenotypes of scrib⁶⁷³/scrib^ird15 transheterozygotes.

Expression of scrib^{Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^69B fully rescues the cell polarization and disc overgrowth phenotypes of scrib⁶⁷³/scrib^ird15 transheterozygotes.

Expression of scrib^{ΔLRR.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^69B fails to rescue the cell polarization and disc overgrowth phenotypes of scrib⁶⁷³/scrib^ird15 transheterozygotes.

Expression of scrib^{L223Q.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^69B fails to rescue the cell polarization and disc overgrowth phenotypes of scrib⁶⁷³/scrib^ird15 transheterozygotes.

Expression of scrib^{ΔLRR.Scer\UAS.P\T.T:Myr6,T:Avic\GFP} under the control of Scer\GAL4^69B fails to rescue the cell polarization and disc overgrowth phenotypes of scrib⁶⁷³/scrib^ird15 transheterozygotes.

Expression of scrib^{4.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^69B fully rescues the cell polarization phenotype but only partially rescues the disc overgrowth phenotype of scrib⁶⁷³/scrib^ird15 transheterozygotes.

Expression of scrib^{ΔPDZs.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^69B fully rescues the cell polarization phenotype but only partially rescues the disc overgrowth phenotype of scrib⁶⁷³/scrib^ird15 transheterozygotes.

Expression of scrib^{ΔPDZ3.4.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^69B fully rescues the cell polarization and disc overgrowth phenotypes of scrib⁶⁷³/scrib^ird15 transheterozygotes.

Co-expression of scrib^{ΔLRR.Scer\UAS.P\T.T:Myr6,T:Avic\GFP} and scrib<up>ΔPDZs.Scer\UAS.P\T. T:Avic \GFP</up> under the control of Scer\GAL4^69B fails to rescue the cell polarization and disc overgrowth phenotypes of scrib⁶⁷³/scrib^ird15 transheterozygotes, even when expression levels are increased via raising larvae at 29 ^oC.

(Zeitler et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a mutation that fails to induce expression of Ecol\lacZ^Dpt.PR normally in response to infection.

(Wu et al., 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

ird15

(Zeitler et al., 2004, Wu et al., 2001)

scrib 3

(Zeitler et al., 2004)

scrib³

(Sharp et al., 2021)

scrib^ird15

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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