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General Information
Symbol
Dmel\scribj7B3
Species
D. melanogaster
Name
FlyBase ID
FBal0010989
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
l(3)j7b3
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The P{lacW} insertion is located int the intron between exons 2 and 3 of scrib.

Insertion components
P{lacW}scribj7B3
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Wing discs from larvae homozygous for scribj7B3 show normal epithelial organisation but are increased in size compared to wild type.

Less than 9% of scrib1/scribj7B3 mutant larvae survive to pupation.

At the third instar stage of larval development, scrib1/scribj7B3 mutants fail to pupate and continue to grow to form giant larvae. The third instar imaginal wings taken from scrib1/scribj7B3 mutants show loss of monolayered structure and growth in three dimensions.

The avoidance response to benzaldehyde of scribj7B3/scribsmi97B adults is not significantly different from that of controls.

scribj7B3/T(Y;3)G75 females lay eggs that do not hatch.

Some scrib882/scribj7B3 animals survive to adulthood.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
Enhancer of
Statement
Reference

scrib[+]/scribj7B3 is an enhancer of visible phenotype of RetMEN2B.GMR

scrib[+]/scribj7B3 is an enhancer of visible phenotype of RetMEN2A.GMR

scrib[+]/scribj7B3 is an enhancer of visible | dominant phenotype of EgfrE1

NOT Enhancer of
Statement
Reference

scribj7B3 is a non-enhancer of visible phenotype of upd1GMR.PB

NOT Suppressor of
Statement
Reference

scrib[+]/scribj7B3 is a non-suppressor of visible phenotype of CycEJP

scribj7B3 is a non-suppressor of visible phenotype of upd1GMR.PB

Phenotype Manifest In
Enhancer of
Statement
Reference

scrib[+]/scribj7B3 is an enhancer of eye phenotype of RetMEN2B.GMR

scrib[+]/scribj7B3 is an enhancer of eye phenotype of RetMEN2A.GMR

scrib[+]/scribj7B3 is an enhancer of eye phenotype of EgfrE1

NOT Enhancer of
Statement
Reference

scribj7B3 is a non-enhancer of eye phenotype of upd1GMR.PB

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

scrib[+]/scribj7B3 is a non-suppressor of eye phenotype of CycEJP

scribj7B3 is a non-suppressor of eye phenotype of upd1GMR.PB

Other
Additional Comments
Genetic Interactions
Statement
Reference

The trachea of sinunwu7 scribj7B3 double mutants have the same phenotype as sinunwu7 single mutants.

Xenogenetic Interactions
Statement
Reference

Ubiquitous expression of Hsap\ScribScer\UAS.T:Avic\GFP-EGFP (under the control of Scer\GAL4Act.PU) effectively suppresses the larval overgrowth phenotype found in scrib1/scribj7B3 mutants. Expression of Hsap\ScribScer\UAS.T:Avic\GFP-EGFP in scrib1/scribj7B3 mutant third instar imaginal wings suppresses the tumorous overgrowth of the epithelial tissues and allows the formation of a monolayered epithelial structure closely resembling that of wild-type discs.

Expression of Hsap\ScribScer\UAS.T:Avic\GFP-EGFP (under the control of Scer\GAL4Act.PU) rescues larval lethality in scrib1/scribj7B3 mutants to allow 59% to form adult structures (pharate adults) and 23% to eclose as mature adult flies. These adult flies exhibit some developmental defects in the eyes and wings on a proportion of rescued animals as well as ectopic scutellar bristles, suggesting the rescue effect is not fully penetrant. Both sexes appear to be sterile.

Complementation and Rescue Data
Comments

Expression of scribΔLRR.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL469B fails to rescue the cell polarization and disc overgrowth phenotypes of scrib673/scribj7B3 transheterozygotes.

Expression of scribΔLRR.Scer\UAS.P\T.T:Myr6,T:Avic\GFP under the control of Scer\GAL469B fails to rescue the cell polarization and disc overgrowth phenotypes of scrib673/scribj7B3 transheterozygotes.

Expression of scrib4.Scer\UAS.P\T.T:Avic\GFP or scribΔPDZs.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL469B provides a more complete rescue of the size phenotype of scrib673/scribj7B3 transheterozygous discs compared to scrib null discs. Increasing the level of transgene expression via raising larvae at 29 oC further increases this rescue.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
References (24)