FlyBase Allele Report: Dmel\scrib[j7B3]

(Tan et al., 2012, Ganguly et al., 2003, Li et al., 2001, Bilder and Perrimon, 2000, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA with Ras85D^V12.UAS

(Waghmare et al., 2024, Pagliarini and Xu, 2003)

CEA with Hsap\SCRIB^UAS.EGFP

(Dow et al., 2003)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates viral infectious disease

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

ameliorates infectious mononucleosis

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

ameliorates Epstein-Barr virus hepatitis

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity | larval stage (with scrib¹)

(Dow et al., 2003)

abnormal size | recessive

(Zeitler et al., 2004)

lethal | recessive

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

lethal - all die before end of larval stage (with scrib¹)

(Dow et al., 2003)

some die during larval stage (with scrib¹)

(Dow et al., 2003)

Phenotype Manifest In

egg | maternal effect (with T(Y;3)G75)

(Cook and Carpenter, 2002)

organism | third instar larval stage (with scrib¹)

(Dow et al., 2003)

wing disc

(Zeitler et al., 2004)

wing disc | third instar larval stage (with scrib¹)

(Dow et al., 2003)

Detailed Description

Statement

Reference

Wing discs from larvae homozygous for scrib^j7B3 show normal epithelial organisation but are increased in size compared to wild type.

(Zeitler et al., 2004)

Less than 9% of scrib¹/scrib^j7B3 mutant larvae survive to pupation.

At the third instar stage of larval development, scrib¹/scrib^j7B3 mutants fail to pupate and continue to grow to form giant larvae. The third instar imaginal wings taken from scrib¹/scrib^j7B3 mutants show loss of monolayered structure and growth in three dimensions.

(Dow et al., 2003)

The avoidance response to benzaldehyde of scrib^j7B3/scrib^smi97B adults is not significantly different from that of controls.

(Ganguly et al., 2003)

scrib^j7B3/T(Y;3)G75 females lay eggs that do not hatch.

(Cook and Carpenter, 2002)

Some scrib⁸⁸²/scrib^j7B3 animals survive to adulthood.

(Wu et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

scrib¹/scrib^j7B3 has lethal | larval stage phenotype, suppressible by Scer\GAL4^Act.PU/Hsap\SCRIB^UAS.EGFP

(Dow et al., 2003)

Enhancer of

Statement

Reference

scrib[+]/scrib^j7B3 is an enhancer of visible phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

scrib[+]/scrib^j7B3 is an enhancer of visible phenotype of Ret^MEN2A.GMR

(Read et al., 2005)

scrib[+]/scrib^j7B3 is an enhancer of visible | dominant phenotype of Egfr^E1

(Read et al., 2005)

NOT Enhancer of

Statement

Reference

scrib^j7B3 is a non-enhancer of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

NOT Suppressor of

Statement

Reference

scrib[+]/scrib^j7B3 is a non-suppressor of visible phenotype of CycE^JP

(Brumby et al., 2004)

scrib^j7B3 is a non-suppressor of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

Phenotype Manifest In

Enhancer of

Statement

Reference

scrib[+]/scrib^j7B3 is an enhancer of eye phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

scrib[+]/scrib^j7B3 is an enhancer of eye phenotype of Ret^MEN2A.GMR

(Read et al., 2005)

scrib[+]/scrib^j7B3 is an enhancer of eye phenotype of Egfr^E1

(Read et al., 2005)

NOT Enhancer of

Statement

Reference

scrib^j7B3 is a non-enhancer of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

Suppressor of

Statement

Reference

scrib^j7B3 is a suppressor of eye phenotype of EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

NOT Suppressor of

Statement

Reference

scrib[+]/scrib^j7B3 is a non-suppressor of eye phenotype of CycE^JP

(Brumby et al., 2004)

scrib^j7B3 is a non-suppressor of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

Other

Statement

Reference

Hsap\SCRIB^UAS.EGFP, Scer\GAL4^Act.PU, scrib¹/scrib^j7B3 has wing phenotype

(Dow et al., 2003)

Hsap\SCRIB^UAS.EGFP, Scer\GAL4^Act.PU, scrib¹/scrib^j7B3 has eye phenotype

(Dow et al., 2003)

Hsap\SCRIB^UAS.EGFP, Scer\GAL4^Act.PU, scrib¹/scrib^j7B3 has scutellar bristle | ectopic phenotype

(Dow et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The trachea of sinu^nwu7 scrib^j7B3 double mutants have the same phenotype as sinu^nwu7 single mutants.

(Wu et al., 2004)

Xenogenetic Interactions

Statement

Reference

Ubiquitous expression of Hsap\Scrib^{Scer\UAS.T:Avic\GFP-EGFP} (under the control of Scer\GAL4^Act.PU) effectively suppresses the larval overgrowth phenotype found in scrib¹/scrib^j7B3 mutants. Expression of Hsap\Scrib^{Scer\UAS.T:Avic\GFP-EGFP} in scrib¹/scrib^j7B3 mutant third instar imaginal wings suppresses the tumorous overgrowth of the epithelial tissues and allows the formation of a monolayered epithelial structure closely resembling that of wild-type discs.

Expression of Hsap\Scrib^{Scer\UAS.T:Avic\GFP-EGFP} (under the control of Scer\GAL4^Act.PU) rescues larval lethality in scrib¹/scrib^j7B3 mutants to allow 59% to form adult structures (pharate adults) and 23% to eclose as mature adult flies. These adult flies exhibit some developmental defects in the eyes and wings on a proportion of rescued animals as well as ectopic scutellar bristles, suggesting the rescue effect is not fully penetrant. Both sexes appear to be sterile.

(Dow et al., 2003)

Complementation and Rescue Data

Complements

scrib^smi97B

(Ganguly et al., 2003)

scrib^smi97B

(Ganguly et al., 2001)

Fails to complement

scrib^63S15

(Brumby et al., 2004)

scrib^ird15

(Wu et al., 2001)

Partially rescued by

scrib^j7B3/scrib⁶⁷³ is partially rescued by scrib^{ΔPDZs.UASp.GFP}/Scer\GAL4^69B

(Zeitler et al., 2004)

scrib^j7B3/scrib⁶⁷³ is partially rescued by scrib^4.UASp.GFP/Scer\GAL4^69B

(Zeitler et al., 2004)

Not rescued by

scrib^j7B3/scrib⁶⁷³ is not rescued by scrib^{ΔLRR.UASp.GFP}/Scer\GAL4^69B

(Zeitler et al., 2004)

scrib^j7B3/scrib⁶⁷³ is not rescued by Scer\GAL4^69B/scrib^{ΔLRR.UASp.Tag:Myr(Src42A),GFP}

(Zeitler et al., 2004)

Comments

Expression of scrib^{ΔLRR.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^69B fails to rescue the cell polarization and disc overgrowth phenotypes of scrib⁶⁷³/scrib^j7B3 transheterozygotes.

Expression of scrib^{ΔLRR.Scer\UAS.P\T.T:Myr6,T:Avic\GFP} under the control of Scer\GAL4^69B fails to rescue the cell polarization and disc overgrowth phenotypes of scrib⁶⁷³/scrib^j7B3 transheterozygotes.

Expression of scrib^{4.Scer\UAS.P\T.T:Avic\GFP} or scrib^{ΔPDZs.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^69B provides a more complete rescue of the size phenotype of scrib⁶⁷³/scrib^j7B3 transheterozygous discs compared to scrib null discs. Increasing the level of transgene expression via raising larvae at 29 ^oC further increases this rescue.

(Zeitler et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

12159

y¹ w^*; P{lacW}scrib^j7B3/TM3, Sb¹

Kyoto

111422

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}82B P{lacW}scrib^j7B3/TM6B, P{Car20y}TPN1, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

l(3)J7B3^j7B3

(Wu et al., 2001)

l(3)j7B3

(Spradling et al., 1999)

l(3)j7B3^j7B3

(Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)

l(3)j7b3

(Brumby et al., 2004)

scrib j7B3

(Zeitler et al., 2004)

scrib^j7B3

(Sotillos et al., 2018, Tan et al., 2012, Goossens et al., 2011, Christensen et al., 2009.2.28, Mao and Freeman, 2009, Dow et al., 2003)

scrib^j7B4

(Bach et al., 2003)

scrib^j7b3

(Waghmare et al., 2024, Cheng et al., 2016, Adamson and Lajeunesse, 2012)

Name Synonyms

Secondary FlyBase IDs

References (26)

Report Sections

Open Close