Ubiquitous expression of Hsap\ScribScer\UAS.T:Avic\GFP-EGFP (under the control of Scer\GAL4Act.PU) effectively suppresses the larval overgrowth phenotype found in scrib1/scribj7B3 mutants. Expression of Hsap\ScribScer\UAS.T:Avic\GFP-EGFP in scrib1/scribj7B3 mutant third instar imaginal wings suppresses the tumorous overgrowth of the epithelial tissues and allows the formation of a monolayered epithelial structure closely resembling that of wild-type discs.
Expression of Hsap\ScribScer\UAS.T:Avic\GFP-EGFP (under the control of Scer\GAL4Act.PU) rescues larval lethality in scrib1/scribj7B3 mutants to allow 59% to form adult structures (pharate adults) and 23% to eclose as mature adult flies. These adult flies exhibit some developmental defects in the eyes and wings on a proportion of rescued animals as well as ectopic scutellar bristles, suggesting the rescue effect is not fully penetrant. Both sexes appear to be sterile.
Expression of scrib4.Scer\UAS.P\T.T:Avic\GFP or scribΔPDZs.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL469B provides a more complete rescue of the size phenotype of scrib673/scribj7B3 transheterozygous discs compared to scrib null discs. Increasing the level of transgene expression via raising larvae at 29 oC further increases this rescue.