FB2025_05 , released December 11, 2025
Allele: Dmel\NmnatWR.UAS.PD
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General Information
Symbol
Dmel\NmnatWR.UAS.PD
Species
D. melanogaster
Name
FlyBase ID
FBal0198137
Feature type
allele
Associated gene
Dmel\Nmnat
Associated Insertion(s)
Carried in Construct
P{UAS-Nmnat.PD.WR}
Key Links
Transgenic product class
loss_of_function_variant
(Zhai et al., 2006)
missense_variant
(Zhai et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
loss_of_function_variant
(Zhai et al., 2006)
missense_variant
(Zhai et al., 2006)
Mutagen
in vitro construct
(Zhai et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-Nmnat.PD.WR}
Cytology
Description

UASt regulatory sequences drive expression of the AT23490 cDNA, in which two key residues required for substrate binding have been mutated (amino acid substitutions W98G and R224A). This results in an enzymatically inactive form of Nmnat.

(Zhai et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Nmnat
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  autosomal dominant cerebellar ataxia
      modeled by Atx-1UAS.cTa
      (Zhai et al., 2008)
      ameliorates  neurodegenerative disease
      modeled by Hsap\ATXN182Q.UAS
      (Zhai et al., 2008)
      ameliorates  tauopathy
      modeled by Hsap\MAPTR406W.UAS
      (Ma et al., 2020)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of NmnatWR.Scer\UAS under the control of Scer\GAL4elav.PU has no effect on learning and memory in an aversive phototaxis assay at 20 days post eclosion. No locomotor activity are seen, as measured by climbing performance and brains do not display any morphological defects.

      (Ali et al., 2012)

      Expression of NmnatWR.Scer\UAS under the control of Scer\GAL4GMR.PF protects flies against neuronal degeneration in response to intense light exposure; eyes expressing this transgene show less vacuolization and a higher number of rhabdomeres than wild-type flies in response to 30 days of light exposure.

      (Zhai et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      NmnatWR.UAS.PD, Scer\GAL4elav.PU is a suppressor of abnormal learning | adult stage phenotype of Hsap\MAPTUAS.cWa, Scer\GAL4elav.PU

      (Ali et al., 2012)

      NmnatWR.UAS.PD, Scer\GAL4elav.PU is a suppressor of abnormal memory | adult stage phenotype of Hsap\MAPTUAS.cWa, Scer\GAL4elav.PU

      (Ali et al., 2012)

      NmnatWR.UAS.PD, Scer\GAL4elav.PU is a suppressor of abnormal neuroanatomy | progressive phenotype of Hsap\MAPTUAS.cWa, Scer\GAL4elav.PU

      (Ali et al., 2012)

      NmnatWR.UAS.PD, Scer\GAL4elav.PU is a suppressor of abnormal learning | adult stage phenotype of Hsap\MAPTR406W.UAS, Scer\GAL4elav.PU

      (Ali et al., 2012)

      NmnatWR.UAS.PD, Scer\GAL4elav.PU is a suppressor of abnormal memory | adult stage phenotype of Hsap\MAPTR406W.UAS, Scer\GAL4elav.PU

      (Ali et al., 2012)

      NmnatWR.UAS.PD, Scer\GAL4elav.PU is a suppressor of abnormal neuroanatomy | progressive phenotype of Hsap\MAPTR406W.UAS, Scer\GAL4elav.PU

      (Ali et al., 2012)

      NmnatWR.UAS.PD, Scer\GAL4elav.PU is a suppressor of increased cell death phenotype of Hsap\MAPTR406W.UAS, Scer\GAL4elav.PU

      (Ali et al., 2012)

      NmnatWR.UAS.PD, Scer\GAL4elav.PU is a suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\MAPTR406W.UAS, Scer\GAL4elav.PU

      (Ali et al., 2012)

      NmnatWR.UAS.PD, Scer\GAL4GMR.PF is a suppressor of abnormal neuroanatomy phenotype of Atx-1UAS.cTa, Scer\GAL4GMR.PF

      (Zhai et al., 2008)

      NmnatWR.UAS.PD, Scer\GAL4GMR.PF is a suppressor of abnormal neuroanatomy phenotype of Hsap\ATXN182Q.UAS, Scer\GAL4GMR.PF

      (Zhai et al., 2008)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of NmnatWR.Scer\UAS under the control of Scer\GAL4elav-C155 protects both wild-type and milt33-853 motor neuron clones from degeneration after axotomy at 16 hours after crushing.

      (Kitay et al., 2013)

      Co-expression of NmnatWR.Scer\UAS suppresses the rhabdomere degeneration phenotype induced by Scer\GAL4GMR.PF>Atx-1Scer\UAS.cTa.

      (Zhai et al., 2008)

      The loss of rhabdomere phenotype of rdgAunspecified mutants is significantly rescued by either NmnatScer\UAS.cZa or NmnatWR.Scer\UAS, under the control of Scer\GAL4GMR.PF. The size of vacuoles that are present in the retina and photoreceptors of rdgAunspecified mutants are reduced by expression of NmnatWR.Scer\UAS, but not NmnatScer\UAS.cZa.

      The number of rhabdomeres per ommatidium is significantly rescued in trpP365 flies by expression of either NmnatScer\UAS.cZa or NmnatWR.Scer\UAS under the control of Scer\GAL4GMR.PF. However, the vacuolarization that occurs in trpP365 retinae and photoreceptors is not suppressed by either transgene.

      (Zhai et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference

      Expression of NmnatWR.Scer\UAS suppresses the learning and memory deficits seen in 20 day old flies expressing Hsap\MAPTScer\UAS.cWa under the control of Scer\GAL4elav.PU. The climbing defects are partially suppressed. The climbing defects are also suppressed. The brain vacuolisation seen at 20 days post eclosion is almost completely suppressed.

      Expression of NmnatWR.Scer\UAS suppresses the learning and memory deficits seen in 20 day old flies expressing Hsap\MAPTR406W.Scer\UAS under the control of Scer\GAL4elav.PU. The climbing defects are also suppressed. The brain vacuolisation seen at 20 days post eclosion is almost completely suppressed and fewer apoptotic cells are seen in the midbrain.

      (Ali et al., 2012)

      The rough eye and rhabdomere phenotypes of Scer\GAL4GMR.PF>Hsap\ATXN182Q.Scer\UAS are partially suppressed by co-expression of NmnatWR.Scer\UAS.

      (Zhai et al., 2008)
      Complementation and Rescue Data
      Rescues

      Scer\GAL421-7/NmnatWR.UAS.PD rescues NmnatΔ4790-2

      (Wen et al., 2011)

      Scer\GAL4GMR.PF/NmnatWR.UAS.PD rescues Nmnat1

      (Zhai et al., 2006)
      Fails to rescue

      Scer\GAL4Act5C.PI/NmnatWR.UAS.PD fails to rescue Nmnat1

      (Zhai et al., 2006)
      Comments

      Post-synaptic expression of the enzymatically-inactive NmnatWR.Scer\UAS transgene, under the control of Scer\GAL421-7 is sufficient to rescue the dendritic phenotypes observed in NmnatΔ4790-2 ddaC clones, demonstrating a cell-autonomous function for Nmnat in the proper maintenance of class IV dendrites.

      (Wen et al., 2011)

      Expression of NmnatWR.Scer\UAS in Nmnat1 mutant photoreceptors, driven by Scer\GAL4GMR.PF, fully rescues synaptic structures and mostly restores rhabdomere size. However, expression of this transgene in Nmnat1 whole organisms, driven by Scer\GAL4Act5C.PI, fails to rescue the lethality.

      (Zhai et al., 2006)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      NmnatWR.Scer\UAS
      NmnatWR.UAS.PD
      NmnatWR.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (6)