FlyBase Allele Report: Dmel\ND-15[GD4800]

General Information

Symbol

Dmel\ND-15^GD4800

Species

D. melanogaster

Name

FlyBase ID

FBal0199441

Feature type

allele

Associated gene

Dmel\ND-15

Associated Insertion(s)

Carried in Construct

P{GD4800}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4800}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4800

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Scer\GAL4^da.G32

(Vilain et al., 2012)

lethal | heat sensitive, with Scer\GAL4^da.G32

(Zhang et al., 2013)

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Flies expressing CG11455^GD4800 under the control of Scer\GAL4^Mef2.PR show dramatically reduced Complex I activity as compared to controls.

(Klein et al., 2014)

Expression of CG11455^GD4800 under the control of Scer\GAL4^da.G32 results in mild flight defects when assayed under "stringent" testing conditions.

(Vilain et al., 2012)

Adults expressing ND-15^GD4800 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 80-90% of the Scer\GAL4^pnr-MD237 expression domain.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4800

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ND-15^GD4800, Scer\GAL4^da.G32 has lethal | heat sensitive phenotype, suppressible by Hsap\TRAP1^UAS.cBa, Scer\GAL4^da.G32

(Zhang et al., 2013)

ND-15^GD4800, Scer\GAL4^da.G32 has abnormal flight phenotype, suppressible by Scer\NDI1^UAS.cVa, Scer\GAL4^da.G32

(Vilain et al., 2012)

NOT suppressed by

Statement

Reference

ND-15^GD4800, Scer\GAL4^da.G32 has lethal | heat sensitive phenotype, non-suppressible by Hsap\TRAP1^D158N.UAS, Scer\GAL4^da.G32

(Zhang et al., 2013)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Ret^{MEN2B.Scer\UAS} does not suppress the reduction in Complex I activity seen when CG11455^GD4800 is expressed under the control of Scer\GAL4^Mef2.PR.

(Klein et al., 2014)

Xenogenetic Interactions

Statement

Reference

The mild flight defects observed under "stringent" testing conditions in flies expressing CG11455^GD4800 under the control of Scer\GAL4^da.G32 are suppressed by co-expression of Scer\NDI1^Scer\UAS.cVa.

(Vilain et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v12838

w¹¹¹⁸; P{GD4800}v12838

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4800

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG11455^GD4800

ND-15^GD4800

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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