FlyBase Allele Report: Dmel\RIOK2[GD7407]

General Information

Symbol

Dmel\RIOK2^GD7407

Species

D. melanogaster

Name

FlyBase ID

FBal0199587

Feature type

allele

Associated gene

Dmel\RIOK2

Associated Insertion(s)

Carried in Construct

P{GD7407}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD7407}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD7407

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates brain glioma

modeled by btl::Egfr^λ.UAS, Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Read et al., 2013)

modeled by Hsap\EGFR^Δ.UAS

(Read et al., 2013)

modeled by Hsap\EGFR^Δ.UAS, Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Read et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression of RIOK2^GD7407 under the control of Scer\GAL4^repo.PU has no effect on third instar larval brain size.

(Read et al., 2013)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in a reduction in the average size of neuroblasts together with shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

(Neumüller et al., 2011)

Adults expressing RIOK2^GD7407 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in early larval lethality or late larval lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality or semi-lethality at the pupal stage, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 0% or 30-40% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 10-20% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 60-70% or 90-100% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

7407

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

RIOK2^GD7407, Scer\GAL4^repo.PU is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\EGFR^Δ.UAS, Scer\GAL4^repo.PU

(Read et al., 2013)

RIOK2^GD7407, Scer\GAL4^repo.PU is a suppressor of neoplasia | third instar larval stage phenotype of Hsap\EGFR^Δ.UAS, Scer\GAL4^repo.PU

(Read et al., 2013)

RIOK2^GD7407, Scer\GAL4^repo.PU is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\EGFR^Δ.UAS, Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU

(Read et al., 2013)

RIOK2^GD7407, Scer\GAL4^repo.PU is a suppressor of neoplasia | third instar larval stage phenotype of Hsap\EGFR^Δ.UAS, Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU

(Read et al., 2013)

RIOK2^GD7407, Scer\GAL4^repo.PU is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2013)

RIOK2^GD7407, Scer\GAL4^repo.PU is a suppressor of neoplasia | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2013)

Other

Statement

Reference

Phenotype Manifest In

Suppressor of

Statement

Reference

RIOK2^GD7407, Scer\GAL4^repo.PU is a suppressor of glial cell | increased number | third instar larval stage phenotype of Hsap\EGFR^Δ.UAS, Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU

(Read et al., 2013)

RIOK2^GD7407, Scer\GAL4^repo.PU is a suppressor of larval brain | third instar larval stage phenotype of Hsap\EGFR^Δ.UAS, Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU

(Read et al., 2013)

RIOK2^GD7407, Scer\GAL4^repo.PU is a suppressor of glial cell | increased number | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2013)

RIOK2^GD7407, Scer\GAL4^repo.PU is a suppressor of larval brain | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2013)

RIOK2^GD7407, Scer\GAL4^repo.PU is a suppressor of glial cell | increased number | third instar larval stage phenotype of Hsap\EGFR^Δ.UAS, Scer\GAL4^repo.PU

(Read et al., 2013)

RIOK2^GD7407, Scer\GAL4^repo.PU is a suppressor of larval brain | third instar larval stage phenotype of Hsap\EGFR^Δ.UAS, Scer\GAL4^repo.PU

(Read et al., 2013)

Other

Statement

Reference

Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, RIOK2^GD7407, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS has larval brain | third instar larval stage phenotype

(Read et al., 2013)

Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, RIOK2^GD7407, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS has glial cell | third instar larval stage phenotype

(Read et al., 2013)

Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, RIOK2^GD7407, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS has neuron | third instar larval stage phenotype

(Read et al., 2013)

Hsap\EGFR^Δ.UAS, RIOK2^GD7407, Scer\GAL4^repo.PU has larval brain | third instar larval stage phenotype

(Read et al., 2013)

Hsap\EGFR^Δ.UAS, RIOK2^GD7407, Scer\GAL4^repo.PU has glial cell | third instar larval stage phenotype

(Read et al., 2013)

Hsap\EGFR^Δ.UAS, RIOK2^GD7407, Scer\GAL4^repo.PU has neuron | third instar larval stage phenotype

(Read et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of RIOK2^GD7407 suppresses the glial overgrowth seen when btl::Egfr^{Scer\UAS.T:λ\cI-DD} and Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} are co-expressed under the control of Scer\GAL4^repo.PU. There are few excess glia and remaining glial cells show abnormal development. The resulting brains are smaller in size than controls, due to a reduction in both glial and neuronal cells.

(Read et al., 2013)

Xenogenetic Interactions

Statement

Reference

Expression of RIOK2^GD7407 suppresses the brain enlargement and glial overgrowth seen when Hsap\EGFR^Δ.Scer\UAS is expressed under the control of Scer\GAL4^repo.PU. There are few excess glia and remaining glial cells show abnormal development. The resulting brains are smaller in size than controls, due to a reduction in both glial and neuronal cells.

Expression of RIOK2^GD7407 suppresses the brain enlargement and glial overgrowth seen when Hsap\EGFR^Δ.Scer\UAS and Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} are co-expressed under the control of Scer\GAL4^repo.PU.

(Read et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v47402

w¹¹¹⁸; P{GD7407}v47402/CyO

Notes on Origin