FlyBase Allele Report: Dmel\Efa6[GD14945]

General Information

Symbol

Dmel\Efa6^GD14945

Species

D. melanogaster

Name

FlyBase ID

FBal0202758

Feature type

allele

Associated gene

Dmel\Efa6

Associated Insertion(s)

Carried in Construct

P{GD14945}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD14945}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD14945

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^ato.3.6

(Qu et al., 2019)

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^elav.PLu

(Qu et al., 2019)

abnormal neuroanatomy | P-stage, with Scer\GAL4^ppk.PU

(Bu et al., 2021)

abnormal size | embryonic stage, with Scer\GAL4^elav.PLu

(Qu et al., 2019)

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

abdominal tergite 5 | female, with Scer\GAL4^Tub.PU

(Dembeck et al., 2015)

abdominal tergite 6 | female, with Scer\GAL4^pnr-MD237

(Dembeck et al., 2015)

abdominal tergite 6 | female, with Scer\GAL4^Tub.PU

(Dembeck et al., 2015)

axon | adult stage, with Scer\GAL4^GMR31F10

(Qu et al., 2019)

axon | embryonic stage, with Scer\GAL4^elav.PLu

(Qu et al., 2019)

axon collateral | adult stage | increased number, with Scer\GAL4^ato.3.6

(Qu et al., 2019)

axon microtubule bundle | adult stage, with Scer\GAL4^GMR31F10

(Qu et al., 2019)

axon microtubule bundle | embryonic stage, with Scer\GAL4^elav.PLu

(Qu et al., 2019)

dendrite | P-stage, with Scer\GAL4^ppk.PU

(Bu et al., 2021)

embryonic/larval sensory neuron | P-stage, with Scer\GAL4^ppk.PU

(Bu et al., 2021)

lobula columnar neuron LC14 | adult stage, with Scer\GAL4^ato.3.6

(Qu et al., 2019)

medulla | adult stage, with Scer\GAL4^ato.3.6

(Qu et al., 2019)

medulla | adult stage, with Scer\GAL4^GMR31F10

(Qu et al., 2019)

Detailed Description

Statement

Reference

Cultured neurons from stage 11 embryos that express Efa6^GD14945 under the control of Scer\GAL4^elav.PLu show a small but significant increase in axon length, and axonal microtubules are disorganized, as compared to controls.

The expression of Efa6^GD14945 under the control of Scer\GAL4^ato.3.6 leads to dorsal cluster neurons show a significantly higher density of axonal branches in the adult medulla.

The expression of Efa6^GD14945 under the control of Scer\GAL4^GMR31F10 results in axons in the adult medulla showing swellings and microtubule bundle disorganization.

(Qu et al., 2019)

Expression of Efa6^GD14945 RNAi under the control of Scer\GAL4^pnr-MD237 results in significantly increased level of pigmentation on the abdominal tergite 6 and the expression under Scer\GAL4^tub.PU results in significantly increased pigmentation on both the abdominal tergite 5 and 6 in adult females compared to controls.

(Dembeck et al., 2015)

Adults expressing Efa6^GD14945 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Adults expressing Efa6^GD14945 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development