FlyBase Allele Report: Dmel\Crk[GD8583]

General Information

Symbol

Dmel\Crk^GD8583

Species

D. melanogaster

Name

FlyBase ID

FBal0206688

Feature type

allele

Associated gene

Dmel\Crk

Associated Insertion(s)

Carried in Construct

P{GD8583}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8583}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8583

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^{Q91.ex1.QUAS.mCherry}

(Pearce et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

abnormal sleep | male, with Scer\GAL4^elav-C155

(Maurer et al., 2020)

decreased rate of movement | adult stage | male limited, with Scer\GAL4^elav-C155

(Maurer et al., 2020)

flightless, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

lethal, with Scer\GAL4^Act5C.PI

(Nagaosa et al., 2011)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

astrocyte-like glial cell, with Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

gamma Kenyon cell, with Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

indirect flight muscle cell, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

Detailed Description

Statement

Reference

Expression of Crk^GD8583 under the control of Scer\GAL4^elav-C155 leads to an increase in walking activity in males only. Males show fewer but longer sleep bouts.

(Maurer et al., 2020)

Knockdown of Crk in astrocytes, through expression of Crk^GD8583 under the control of Scer\GAL4^alrm.PD, leads to a 20% reduction in astrocytic vacuole area compared to controls. These animals also exhibit a significant accumulation of brp[+] debris in the SOG and the abdominal region of the ventral nerve cord.

Expression of Crk^GD8583 under the control of Scer\GAL4^alrm.PD results in a mild suppression of mushroom body γ neuron pruning at 18 hours after pupal formation and a mild phenotype in adults.

(Tasdemir-Yilmaz and Freeman, 2014)

The expression of Crk^GD8583 under the control of Scer\GAL4^srp.Hemo does not affect the competence of embryonic hemocytes of undertaking phagocytosis of apoptotic cells, as compared to controls.

(Nagaosa et al., 2011)

Adults expressing Crk^GD8583 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in missing adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in morphologically wild-type myofibrils in the adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in wild-type sarcomeres in the adult indirect flight muscles.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development