FlyBase Allele Report: Dmel\Gad1[GD8508]

General Information

Symbol

Dmel\Gad1^GD8508

Species

D. melanogaster

Name

FlyBase ID

FBal0207086

Feature type

allele

Associated gene

Dmel\Gad1

Associated Insertion(s)

Carried in Construct

P{GD8508}

Also Known As

UAS-Gad1-RNAi, UAS-Gad^RNAi, UAS-GAD-RNAi, UAS-Gad1^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8508}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8508

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates neurofibromatosis

modeled by Nf1^JF01866

(Georganta et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior, with Scer\GAL4^5-HT1B.PY

(Yuan et al., 2014)

abnormal flight, with Scer\GAL4^elav.PLu

(Dietzl et al., 2007)

abnormal locomotor behavior, with Scer\GAL4^elav.PLu

(Dietzl et al., 2007)

abnormal medium-term memory | adult stage, with Scer\GAL4^VT064246

(Muria et al., 2021)

abnormal smell perception, with Scer\GAL4^GH146

(Liu and Davis, 2009)

abnormal touch perception | adult stage, with Scer\GAL4^GMR41E11

(Jeong et al., 2016)

lethal, with Scer\GAL4^elav.PLu

(Dietzl et al., 2007)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

adult mushroom body anterior paired lateral cell, with Scer\GAL4^GH146

(Liu and Davis, 2009)

Detailed Description

Statement

Reference

Temporal expression of Gad1^GD8508 under the control of Scer\GAL4^VT064246 during adulthood (by using Gal80[ts]) decreases olfactory medium term memory (1h) when the experiment is carried out in crowded conditions (groups of 32 flies - socially facilitated memory).

(Muria et al., 2021)

Expression of Gad1^GD8508 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4^GMR41E11 results in defects in sensing food hardness as the flies show diminished preference for softer food to harder food (at the expense of sweetness) compared to controls.

(Jeong et al., 2016)

Expression of Gad1^GD8508 under the control of Scer\GAL4^dimm-929 does not affect sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503). As in wild type flies, courtship defects are seen in response to (S,Z,Z)-CH503.

(Shankar et al., 2015)

Expression of Gad1^GD8508 under the control of Scer\GAL4^5-HT1B.PY impairs the inhibition of female contact-induced male-male aggression seen in wild type males that have previously been exposed to females.

(Yuan et al., 2014)

Adults expressing Gad1^GD8508 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Flies expressing Gad1^GD8508 in the mushroom body anterior paired lateral (MB-APL) neuron, under the control of Scer\GAL4^GH146 show a significant enhancement of performance after olfactory classical conditioning.

Flies expressing Gad1^GD8508 in the mushroom body anterior paired lateral (MB-APL) neuron, under the control of Scer\GAL4^GH146 that also express Scer\GAL80^Cha.PK, which eliminates expression in the MB-APL, fail to show enhanced learning after olfactory classical conditioning.

(Liu and Davis, 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development