FlyBase Allele Report: Scer\GAL4[5-HT1B.PY]

General Information

Symbol

Scer\GAL4^5-HT1B.PY

Species

S. cerevisiae

Name

5-hydroxytryptamine (serotonin) receptor 1B promoter construct of Yuan

FlyBase ID

FBal0406224

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{5-HT1B-GAL4.Y}

Also Known As

5-HT1B-Gal4, 5HT1B-Gal4, 5-HTR1B-GAL4, 1B-Gal4, 5HTR1B-Gal4, d5-HT1B-GAL4, 5-HT_1B-GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Yuan et al., 2005, Yuan et al., 2005)

Progenitor genotype

Carried in construct

P{5-HT1B-GAL4.Y}

Cytology

Description

A 4.3kb upstream regulatory region of 5-HT1B regulates expression of a GAL4 driver.

(Yuan et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

5-HT1B

5-HT1B_d5-HT1B-GAL4

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

neuron | subset

(Zhou et al., 2012)

third instar larval stage & adult stage

brain | restricted

(Luo et al., 2012)

Additional Information

Statement

Reference

ScerGAL4^5-HTR1B drives expression in alpha'/beta' and gamma Kenyon cells that express 5-HT1B.

(Ries et al., 2017)

Drives expression in gamma Kenyon cells in the adult mushroom body.

(FlyBase Curators, 2017)

ScerGAL4^5-HT1B.PY drives expression in the anterior and posterior rings of the ellipsoid body. In the latter, expression is observed in the innermost and in an outer ring. Rings in the midportion of the ellipsoid body and an anterior region of the cortex are also labelled.

(Kahsai et al., 2012)

ScerGAL4^5-HT1B.PY expresses in cells adjacent to (but not a part of) the insulin secreting cells of pars intercerebralis, as well as ellipsoid body and bulb. No colocalization in insulin secreting cells is seen with anti-Ilp2 staining in adults or larvae.

(Luo et al., 2012)

ScerGAL4^5-HTR1B drives expression in neurons expressing serotonin receptor 1B

(Zhou et al., 2012)

Marker for

gamma Kenyon cell

(Wu et al., 2023)

Reflects expression of

5-HT1B

(Bloomington Drosophila Stock Center, 2017, Ries et al., 2017, Kahsai et al., 2012, Luo et al., 2012)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior, with Ctet\tetX^{TNT-LC.UAS.cSa}, Scer\FLP1^fru

(Yuan et al., 2014)

abnormal behavior, with Gad1^GD8508

(Yuan et al., 2014)

abnormal behavior, with Hsap\KCNJ2^UAS.EGFP

(Yuan et al., 2014)

abnormal behavior, with Hsap\KCNJ2^UAS.GFP, Scer\FLP1^fru

(Yuan et al., 2014)

abnormal behavior, with VGAT^KK101500

(Yuan et al., 2014)

abnormal behavior | heat sensitive, with Scer\FLP1^fru, TrpA1^{-FRT.UAS.cvPa}

(Yuan et al., 2014)

abnormal circadian rhythm, with 5-HT1B^RNAi.UAS

(Yuan et al., 2005)

abnormal circadian rhythm, with 5-HT1B^UAS.cYa

(Yuan et al., 2005)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^c739, shi^1.UAS

(Xie et al., 2013)

abnormal memory | adult stage | heat sensitive, with shi^1.UAS

(Xie et al., 2013)

abnormal memory | adult stage | heat sensitive, with shi^{ts1.IVS.p10.20xUAS}

(Shyu et al., 2017)

decreased efficacy of long-term memory | adult stage, with Vajk1^KK111677

(Widmer et al., 2018)

decreased efficacy of short-term memory | adult stage, with Vajk1^KK111677

(Widmer et al., 2018)

Phenotype Manifest In

Detailed Description

Statement

Reference

Inhibition (using shi^{ts1.IVS.p10.20xUAS} at 32[o]C) of Scer\GAL4^5-HT1B.PY cells in adults during retrieval alone, but not acquisition and consolidation, significantly reduces 24-hour associative olfactory water-reward memory, compared to controls; inhibition has no effect on odor acuity or water preference, compared to controls.

(Shyu et al., 2017)

Inhibition (using shi^1.UAS at 33[o]C) of Scer\GAL4^5-HT1B.PY cells in adults during testing significantly reduces 3 min, 1 h and 3 h aversive or appetitive olfactory memory, compared to controls; reduction in 3 h aversive or appetitive memory, and 1 h appetitive memory, is no longer seen when inhibition is restricted to a smaller subset of Scer\GAL4^5-HT1B.PY cells using Scer\GAL80^Mef2.mb247; inhibition of the combined set of Scer\GAL4^5-HT1B.PY cells and Scer\GAL4^c739 significantly reduces 3 h and 3 min aversive or appetitive memory, compared to controls; inhibition of Scer\GAL4^5-HT1B.PY cells has no effect on naive preferences for odors, shock or sugar, compared to controls.