FlyBase Allele Report: Dmel\hig[UAS.GFP]

General Information

Symbol

Dmel\hig^UAS.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0299599

Feature type

allele

Associated gene

Dmel\hig

Associated Insertion(s)

Carried in Construct

P{UAS-hig.GFP}

Key Links

Transgenic product class

cDNA

(Nakayama et al., 2014)

wild_type

(Nakayama et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Nakayama et al., 2014)

wild_type

(Nakayama et al., 2014)

Mutagen

in vitro construct

(Nakayama et al., 2014)

Progenitor genotype

Carried in construct

P{UAS-hig.GFP}

Cytology

Description

UASt regulatory sequences drive expression of a cDNA of the longest hig isoform tagged with GFP.

(Nakayama et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

hig

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^elav.PU/hig^UAS.GFP rescues hig^dd37

(Nakayama et al., 2014)

hig^UAS.GFP/Scer\GAL4^ChAT.PU rescues hig^dd37

(Nakayama et al., 2014)

hig^UAS.GFP/Scer\GAL4^VGlut1.PU rescues hig^dd37

(Nakayama et al., 2014)

hig^UAS.GFP/Scer\GAL4^VGlut1-OK371 rescues hig^dd37

(Nakayama et al., 2014)

hig^UAS.GFP/Scer\GAL4^Eaat1.PU rescues hig^dd37

(Nakayama et al., 2014)

hig^UAS.GFP/Scer\GAL4^repo.PU rescues hig^dd37

(Nakayama et al., 2014)

hig^UAS.GFP/Scer\GAL4^Ddc.PL rescues hig^dd37

(Nakayama et al., 2014)

Fails to rescue

Scer\GAL4^5-HT1B.PY/hig^UAS.GFP fails to rescue hig^dd37

(Nakayama et al., 2014)

Comments

Expression of hig^{Scer\UAS.T:Avic\GFP} under the control of either Scer\GAL4^elav.PU, Scer\GAL4^Cha.PU, Scer\GAL4^VGlut.PU, Scer\GAL4^VGlut-OK371, Scer\GAL4^Eaat1.PU or Scer\GAL4^repo.PU rescues the reduced longevity of hig^dd37 homozygotes.

Expression of hig^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^5-HT1B.PY does not rescue the reduced longevity of hig^dd37 homozygotes.

Expression of two copies (but not one copy) of hig^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^Ddc.PL rescues the reduced longevity of hig^dd37 homozygotes.

(Nakayama et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

hig^{Scer\UAS.T:Avic\GFP}

hig^UAS.GFP

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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