FlyBase Allele Report: Dmel\hig[dd37]

General Information

Symbol

Dmel\hig^dd37

Species

D. melanogaster

Name

FlyBase ID

FBal0031492

Feature type

allele

Associated gene

Dmel\hig

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

loss of function allele

amorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Hoshino et al., 1996)

amorphic allele - molecular evidence

(Nakayama et al., 2014)

loss of function allele

(Hoshino et al., 1993)

Mutagen

Delta2-3 transposase

(Hoshino et al., 1993)

Progenitor genotype

hig^P1

(Hoshino et al., 1993)

Cytology

Description

Imprecise excision of the P{FZ} insert of hig^P1 causing a 10kb deletion within hig and loss of the insertion.

(Hoshino et al., 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

hig transcripts are undetectable by in situ hybridization in hig^dd37 embryos.

(Sanchez-Herrero and Akam, 1989)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior

(Hoshino et al., 1996)

abnormal behavior | recessive

(Hoshino et al., 1993)

chemical resistant

(Nakayama et al., 2014)

flightless

(Hoshino et al., 1996)

short lived

(Nakayama et al., 2016, Nakayama et al., 2014)

uncoordinated | larval stage

(Hoshino et al., 1993)

Phenotype Manifest In

central nervous system

(Hoshino et al., 1996)

Detailed Description

Statement

Reference

Homozygous adults are short lived (they all die by approximately 10 days after eclosion).

(Nakayama et al., 2016)

Mutant flies show increased resistance to the nAChRα6 agonist spinosad compared to wild-type controls.

(Nakayama et al., 2014)

Homozygotes cannot fly or jump, they can only walk slowly with an unstable gait, sometimes when standing still they exhibit body tremors. In most mutants the DLMs exhibit frequent bursting activity due to defects in the CNS.

(Hoshino et al., 1996)

Semi-lethal, only 10% of first instar larvae survive to adulthood and these are less abnormal than hig^P1. Larval locomotor activity is uncoordinated. hig^dd37 homozygous larvae are stubby.

(Hoshino et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

hig^dd37 is rescued by Scer\GAL4^elav.PU/hig^UAS.GFP

(Nakayama et al., 2014)

hig^dd37 is rescued by hig^UAS.GFP/Scer\GAL4^ChAT.PU

(Nakayama et al., 2014)

hig^dd37 is rescued by hig^UAS.GFP/Scer\GAL4^VGlut1.PU

(Nakayama et al., 2014)

hig^dd37 is rescued by hig^UAS.GFP/Scer\GAL4^VGlut1-OK371

(Nakayama et al., 2014)

hig^dd37 is rescued by hig^UAS.GFP/Scer\GAL4^Eaat1.PU

(Nakayama et al., 2014)

hig^dd37 is rescued by hig^UAS.GFP/Scer\GAL4^repo.PU

(Nakayama et al., 2014)

hig^dd37 is rescued by hig^UAS.GFP/Scer\GAL4^Ddc.PL

(Nakayama et al., 2014)

hig^dd37 is rescued by hig^D12.hs

(Hoshino et al., 1996)

Partially rescued by

hig^dd37 is partially rescued by hig^D12.hs

(Hoshino et al., 1993)

Not rescued by

hig^dd37 is not rescued by Scer\GAL4^5-HT1B.PY/hig^UAS.GFP

(Nakayama et al., 2014)

Comments

Heat shock of hig^D12.hs during pupariation rescues hig^dd37 defective adult locomotion. rescued adults can jump, fly and do not exhibit body tremors.

(Hoshino et al., 1996)

hig^D12.hs rescues more than 30% of hig^dd37 flies to adults and exhibits partial rescue of the abnormal locomotion.

(Hoshino et al., 1993)

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

109573

w^*; hig^dd37 / CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

hig^dd37

(Nakayama et al., 2023, Nakayama et al., 2016, Nakayama et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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