FlyBase Allele Report: Dmel\Hsp27[GD11327]

General Information

Symbol

Dmel\Hsp27^GD11327

Species

D. melanogaster

Name

FlyBase ID

FBal0207339

Feature type

allele

Associated gene

Dmel\Hsp27

Associated Insertion(s)

Carried in Construct

P{GD11327}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11327}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11327

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates neurodegenerative disease

modeled by Zzzz\CAG^{41Q.UAS.Tag:HA}

(Chen et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

short lived, with Scer\GAL4^da.PU

(Chen et al., 2012)

short lived, with Scer\GAL4^hs.PU

(Chen et al., 2012)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^GMR.PU

(Chen et al., 2012)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PU

(Chen et al., 2012)

ommatidium, with Scer\GAL4^GMR.PU

(Chen et al., 2012)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression of Hsp27^GD11327 under the control of either Scer\GAL4^hs.PU or Scer\GAL4^da.PU results in short lived adults compared to controls

(Chen et al., 2012)

Adults expressing Hsp27^GD11327 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 30-40% of the Scer\GAL4^pnr-MD237 expression domain.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11327

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Hsp27^GD11327, Scer\GAL4^GMR.PU has visible phenotype, suppressible by Atg7^UAS.cCa, Scer\GAL4^GMR.PU

(Chen et al., 2012)

Enhancer of

Statement

Reference

Hsp27^GD11327, Scer\GAL4^GMR.PU is an enhancer of visible phenotype of Scer\GAL4^GMR.PU, Zzzz\CAG^{41Q.UAS.Tag:HA}

(Chen et al., 2012)

NOT Enhancer of

Statement

Reference

Hsp27^GD11327, Scer\GAL4^GMR.PU is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PU, Zzzz\CAG^{63Q.UAS.Tag:HA}

(Chen et al., 2012)

Other

Statement

Reference

Atg7^UAS.cCa, Hsp27^GD11327, Scer\GAL4^Appl.PU has long lived phenotype

(Chen et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Hsp27^GD11327, Scer\GAL4^GMR.PU has eye phenotype, suppressible by Atg7^UAS.cCa, Scer\GAL4^GMR.PU

(Chen et al., 2012)

Hsp27^GD11327, Scer\GAL4^GMR.PU has ommatidium phenotype, suppressible by Atg7^UAS.cCa, Scer\GAL4^GMR.PU

(Chen et al., 2012)

Enhancer of

Statement

Reference

Hsp27^GD11327, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Scer\GAL4^GMR.PU, Zzzz\CAG^{41Q.UAS.Tag:HA}

(Chen et al., 2012)

NOT Enhancer of

Statement

Reference

Hsp27^GD11327, Scer\GAL4^GMR.PU is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PU, Zzzz\CAG^{63Q.UAS.Tag:HA}

(Chen et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Atg7^Scer\UAS.cCa fully rescues the rough eye phenotype caused by expression of Hsp27^GD11327 under the control of Scer\GAL4^GMR.PU.

(Chen et al., 2012)

Xenogenetic Interactions

Statement

Reference

The mild mosaic eye pigmentation defect caused by expression of Zzzz\CAG^{41Q.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PU is enhanced by co-expression of Hsp27^GD11327.

The rough eye defect caused by expression of Zzzz\CAG^{63Q.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PU is not enhanced by co-expression of Hsp27^GD11327.

(Chen et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v40530

w¹¹¹⁸; P{GD11327}v40530

Notes on Origin

Discoverer