FlyBase Allele Report: Dmel\Itpr[GD1676]

General Information

Symbol

Dmel\Itpr^GD1676

Species

D. melanogaster

Name

FlyBase ID

FBal0207401

Feature type

allele

Associated gene

Dmel\Itpr

Associated Insertion(s)

Carried in Construct

P{GD1676}

Also Known As

UAS-IP3R RNAi, UAS-itpr-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1676}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1676

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease 2

modeled by park¹

(Ham et al., 2023)

ameliorates Parkinson's disease 6

modeled by Pink1^B9

(Ham et al., 2023)

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Al-Ramahi et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

abnormal cell number | P-stage, with Scer\GAL4^Mef2.247

(Zhang et al., 2024)

abnormal cell number | P-stage, with Scer\GAL4^phtm.PO, Scer\GAL80^spok.1.45

(Zhang et al., 2024)

abnormal developmental rate | larval stage, with Scer\GAL4^phtm.PO

(Yamanaka et al., 2015)

abnormal neuroanatomy, with Scer\GAL4²²¹

(Hattori et al., 2013)

abnormal neuroanatomy, with Scer\GAL4^ppk.PG

(Hattori et al., 2013)

increased body size | third instar larval stage, with Scer\GAL4^phtm.PO

(Yamanaka et al., 2015)

partially lethal - majority live, with Scer\GAL4^phtm.PO

(Yamanaka et al., 2015)

some die during larval stage, with Scer\GAL4^phtm.PO

(Yamanaka et al., 2015)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

abdominal dorsal multidendritic neuron ddaC | third instar larval stage, with Scer\GAL4^ppk.PG

(Hattori et al., 2013)

abdominal dorsal multidendritic neuron ddaD | third instar larval stage, with Scer\GAL4²²¹

(Hattori et al., 2013)

abdominal dorsal multidendritic neuron ddaE | third instar larval stage, with Scer\GAL4²²¹

(Hattori et al., 2013)

dendritic tree | third instar larval stage, with Scer\GAL4²²¹

(Hattori et al., 2013)

dendritic tree | third instar larval stage, with Scer\GAL4^ppk.PG

(Hattori et al., 2013)

enteroendocrine cell | P-stage | decreased number, with Scer\GAL4^Mef2.247

(Zhang et al., 2024)

enteroendocrine cell | P-stage | decreased number, with Scer\GAL4^phtm.PO, Scer\GAL80^spok.1.45

(Zhang et al., 2024)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

vesicle | third instar larval stage, with Scer\GAL4^phtm.PO

(Yamanaka et al., 2015)

Detailed Description

Statement

Reference

Knockdown of Itp-r83A^GD1676 (along with UAS-Dcr-2 expression) in the prothoracic gland (driven by Scer\GAL4^phm.PO) leads to growth arrest during larval development (approximately 30% in the first or second stage and 10% in the third instar stage). Arrested third instar larvae also show an overgrowth phenotype due to the extended larval feeding period; feeding ecdysone (E) to these larvae almost fully rescues the arrest phenotype. Those larvae that do initiate pupariation are significantly delayed in doing so and exhibit overgrowth phenotypes (rescued by E feeding). Scer\GAL4^phm.PO>Itp-r83A^GD1676 larvae (during the extended third instar larval stage) show an accumulation of Syt1^{Scer\UAS.T:Avic\GFP-EGFP} vesicles in the cytoplasm (especially adjacent to the plasma membrane). Scer\GAL4^phm.PO>Itp-r83A^GD1676 larvae show normal gross morphology of the prothoracic gland.

(Yamanaka et al., 2015)

Expressing Itp-r83A^GD1676 in the fat body under the control of Scer\GAL4^ppl.PP increases lipid droplet size.

(Bi et al., 2014)

Expression of Itp-r83A^GD1676 results in a significant decrease in the number of dendrite branch terminals in either class I ddaD and ddaE (when expression is driven by Scer\GAL4²²¹, in combination with Dicer-2 for efficient RNAi) or class IV ddaC (when expression is driven by Scer\GAL4^ppk.PG, in combination with Dicer-2 for efficient RNAi) abdominal dorsal multidendritic neurons from third instar larvae, as compared to controls.

(Hattori et al., 2013)

Adults expressing Itp-r83A^GD1676 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 0% or 80-90% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1676

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Itpr^GD1676, Scer\GAL4^phtm.PO has abnormal developmental rate | larval stage phenotype, suppressible | partially by Ras85D^V12.UAS, Scer\GAL4^phtm.PO

(Yamanaka et al., 2015)

Itpr^GD1676, Scer\GAL4^phtm.PO has some die during larval stage phenotype, suppressible | partially by Ras85D^V12.UAS, Scer\GAL4^phtm.PO

(Yamanaka et al., 2015)

Suppressor of

Statement

Reference

Itpr^GD1676/Scer\GAL4^hs.PB is a suppressor | partially of visible | adult stage phenotype of park¹

(Ham et al., 2023)

Itpr^GD1676/Scer\GAL4^hs.PB is a suppressor | partially of decreased rate of adult locomotory behavior phenotype of park¹

(Ham et al., 2023)

Itpr^GD1676/Scer\GAL4^hs.PB is a suppressor | partially of increased cell death | adult stage phenotype of park¹

(Ham et al., 2023)

Itpr^GD1676/Scer\GAL4^hs.PB is a suppressor | partially of decreased cell number | adult stage phenotype of park¹

(Ham et al., 2023)

Itpr^GD1676/Scer\GAL4^hs.PB is a suppressor | partially of decreased rate of adult locomotory behavior phenotype of Pink1^B9

(Ham et al., 2023)

Itpr^GD1676/Scer\GAL4^hs.PB is a suppressor | partially of visible | adult stage phenotype of Pink1^B9

(Ham et al., 2023)

Itpr^GD1676/Scer\GAL4^hs.PB is a suppressor | partially of increased cell death | adult stage phenotype of Pink1^B9

(Ham et al., 2023)

Itpr^GD1676/Scer\GAL4^hs.PB is a suppressor | partially of decreased cell number | adult stage phenotype of Pink1^B9

(Ham et al., 2023)

Itpr^GD1676 is a suppressor | partially of majority die during larval stage phenotype of Gαq^Q203L.UAS, Scer\GAL4^phtm.PO

(Yamanaka et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

Itpr^GD1676/Scer\GAL4^hs.PB is a suppressor | partially of wing phenotype of Pink1^B9

(Ham et al., 2023)

Itpr^GD1676/Scer\GAL4^hs.PB is a suppressor | partially of mitochondrion | adult stage phenotype of Pink1^B9

(Ham et al., 2023)

Itpr^GD1676/Scer\GAL4^hs.PB is a suppressor | partially of flight muscle cell phenotype of Pink1^B9

(Ham et al., 2023)

Itpr^GD1676/Scer\GAL4^hs.PB is a suppressor | partially of adult dopaminergic neuron | decreased number phenotype of Pink1^B9

(Ham et al., 2023)

Itpr^GD1676/Scer\GAL4^hs.PB is a suppressor | partially of adult thorax phenotype of park¹

(Ham et al., 2023)

Itpr^GD1676/Scer\GAL4^hs.PB is a suppressor | partially of wing phenotype of park¹

(Ham et al., 2023)

Itpr^GD1676/Scer\GAL4^hs.PB is a suppressor | partially of mitochondrion | adult stage phenotype of park¹

(Ham et al., 2023)

Itpr^GD1676/Scer\GAL4^hs.PB is a suppressor | partially of flight muscle cell phenotype of park¹

(Ham et al., 2023)

Itpr^GD1676/Scer\GAL4^hs.PB is a suppressor | partially of adult dopaminergic neuron | decreased number phenotype of park¹

(Ham et al., 2023)

Itpr^GD1676/Scer\GAL4^hs.PB is a suppressor | partially of adult thorax phenotype of Pink1^B9

(Ham et al., 2023)

NOT Suppressor of

Statement

Reference

Itpr^GD1676, Scer\GAL4^ppl.PP is a non-suppressor of adult fat body phenotype of Scer\GAL4^ppl.PP, Seipin^null

(Bi et al., 2014)

Itpr^GD1676, Scer\GAL4^ppl.PP is a non-suppressor of lipid droplet phenotype of Scer\GAL4^ppl.PP, Seipin^null

(Bi et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Ras85D^V12.Scer\UAS partially suppresses the developmental arrest at the larval stage and pupariation delay seen in Scer\GAL4^phm.PO>Itp-r83A^GD1676 (along with UAS-Dcr-2) flies.

Co-expression of Itp-r83A^GD1676, Unc-13-4B^GD7394, Rab3^KK108633 or Syt1^KK108653 (along with UAS-Dcr-2 expression) significantly suppresses larval lethality seen in Scer\GAL4^phm.PO>Gαq^{Q203L.Scer\UAS}.

(Yamanaka et al., 2015)

Expression of Itp-r83A^GD1676 under the control of Scer\GAL4^ppl.PP does not rescue the reduction in lipid droplet size seen in Seipin^null mutant fat body cells.

(Bi et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v6484

w¹¹¹⁸; P{GD1676}v6484

v6486

w¹¹¹⁸; P{GD1676}v6486

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1676

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Itp-r83A^GD1676

(Al-Ramahi et al., 2018)

ItpR^RNAi#1

(Weiss et al., 2022)

Itpr^GD1676

Name Synonyms

Secondary FlyBase IDs

References (17)

Report Sections

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