FlyBase Allele Report: Dmel\Mt2[GD5033]

General Information

Symbol

Dmel\Mt2^GD5033

Species

D. melanogaster

Name

FlyBase ID

FBal0207670

Feature type

allele

Associated gene

Dmel\Mt2

Associated Insertion(s)

Carried in Construct

P{GD5033}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD5033}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD5033

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | adult stage, with Scer\GAL4^c564

(Kumar et al., 2016)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible | adult stage, with Scer\GAL4^en.PU

(Dietz et al., 2015)

Phenotype Manifest In

wing, with Scer\GAL4^en.PU

(Dietz et al., 2015)

wing posterior compartment, with Scer\GAL4^en.PU

(Dietz et al., 2015)

wing vein, with Scer\GAL4^en.PU

(Dietz et al., 2015)

Detailed Description

Statement

Reference

The expression of Mt2^GD5033 under the control of Scer\GAL4^c564 has no obvious affect on the inflammatory response to Gram-positive bacterial infection, but leads to a minor ectopic inflammatory response in basal (non-infected) conditions, as compared to controls.

(Kumar et al., 2016)

Expression of Mt2^GD5033 under the control of Scer\GAL4^en.PU results in ectopic vein material in posterior wing compartment. Scer\GAL4^Act5C.PI-driven expression is viable; no overt adult phenotype is observed with any of the following driver: Scer\GAL4^ey.PH, Scer\GAL4^GMR.PF or Scer\GAL4^nub.PK.

(Dietz et al., 2015)

Adults expressing Mt2^GD5033 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

5033

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Mt2^GD5033, Scer\GAL4^en.PU has visible | adult stage phenotype, enhanceable by htt^GD14943, Scer\GAL4^en.PU

(Dietz et al., 2015)

Mt2^GD5033, Scer\GAL4^en.PU has visible | adult stage phenotype, enhanceable by htt^int

(Dietz et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Mt2^GD5033, Scer\GAL4^en.PU has wing phenotype, enhanceable by htt^GD14943, Scer\GAL4^en.PU

(Dietz et al., 2015)

Mt2^GD5033, Scer\GAL4^en.PU has wing phenotype, enhanceable by htt^int

(Dietz et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The adult wing phenotype induced by expression of Mt2^GD5033 under the control of Scer\GAL4^en.PU is enhanced by co-expression of htt^GD14943 or by combination with a single copy of htt^int.

(Dietz et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v37816