FlyBase Allele Report: Dmel\Sac1[GD2238]

General Information

Symbol

Dmel\Sac1^GD2238

Species

D. melanogaster

Name

FlyBase ID

FBal0208468

Feature type

allele

Associated gene

Dmel\Sac1

Associated Insertion(s)

Carried in Construct

P{GD2238}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD2238}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD2238

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^C57

(Forrest et al., 2013)

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav.PU

(Forrest et al., 2013)

increased cell death, with Scer\GAL4^elav.PU

(Forrest et al., 2013)

lethal | pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

partially lethal, with Scer\GAL4^elav.PU

(Forrest et al., 2013)

partially lethal - majority die | pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

short lived, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^elav.PU

(Forrest et al., 2013)

Phenotype Manifest In

eye, with Scer\GAL4^elav.PU

(Forrest et al., 2013)

interommatidial bristle, with Scer\GAL4^elav.PU

(Forrest et al., 2013)

NMJ bouton, with Scer\GAL4^elav.PU

(Forrest et al., 2013)

NMJ bouton | larval stage, with Scer\GAL4^C57

(Forrest et al., 2013)

ommatidium, with Scer\GAL4^elav.PU

(Forrest et al., 2013)

Detailed Description

Statement

Reference

Pan-neuronal expression of Sac1^GD2238 under the control of Scer\GAL4^elav.PU results in a decrease in bouton number and a concomitant increase in their size. An increased number of boutons show microtubule disorganisation compared to controls.

Expression of Sac1^GD2238 in muscle under the control of Scer\GAL4^C57 produces a complex phenotype affecting both the pre- and post-synaptic compartments. An aberrant synaptic morphology is seen, although the number of boutons is normal. Boutons are larger and exhibit a highly irregular shape with a number of spike-like protrusions emerging from their contours.

60% of flies expressing Sac1^GD2238 under the control of Scer\GAL4^elav.PU fail to eclose, and survivors exhibit marked neurodegeneration in the eye. This degeneration is more severe in females than in males, with females exhibiting extensive degpigmentation, whereas males have the majority of the eye surface covered by patches of severe necrosis. Necrosis is observed in 100% of males, but the severity of the phenotype varies. At the ultrastructural level, males have extensive regions of fused ommatidia and missing or misoriented interommatidial bristles. Eye sections show severe disruption in retinal organisation, with thinning of the retina and loss of retinal neurons.

(Forrest et al., 2013)

Adults expressing Sac1^GD2238 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in lethality by 7 days after eclosion.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality or semi-lethality at the pupal stage, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

2238

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Sac1^GD2238, Scer\GAL4^elav.PU has abnormal neuroanatomy phenotype, non-enhanceable by Vap33^GD3990, Scer\GAL4^elav.PU

(Forrest et al., 2013)

NOT Enhancer of

Statement

Reference

Sac1^GD2238, Scer\GAL4^elav.PU is a non-enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^elav.PU, Vap33^GD3990

(Forrest et al., 2013)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Sac1^GD2238, Scer\GAL4^elav.PU has NMJ bouton phenotype, non-enhanceable by Vap33^GD3990, Scer\GAL4^elav.PU

(Forrest et al., 2013)

NOT Enhancer of

Statement

Reference

Sac1^GD2238, Scer\GAL4^elav.PU is a non-enhancer of NMJ bouton phenotype of Scer\GAL4^elav.PU, Vap33^GD3990

(Forrest et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The bouton phenotypes in larvae co-expressing Sac1^GD2238 and Vap-33-1^GD3990 under the control of Scer\GAL4^elav.PU are no more severe than those seen in either mutant alone.

(Forrest et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

VDRC

v37216